Reviewed, UniProtKB/Swiss-Prot Q87Q92 (MAO1_VIBPA)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	VP1258

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000160236
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87Q92-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E1B8D7A80CF073F3	FASTA	562	62,299
	10 20 30 40 50 60 MNNDKRPLYI PYAGPALMAT PLLNKGSAFS AEERSSFNLE GLLPETTETI QEQVERAYQQ 70 80 90 100 110 120 YKSFESDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR 130 140 150 160 170 180 GLFISYPNRD RIDDLLNNAA NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAYTLPI VLDVGTNNPQ RLADPMYMGW RHPRITGPDY DAFVEEFIQA VQRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKDR ICCFNDDIQG TAAVTVGSLL AACKAAGTQL SKQRITFLGA 310 320 330 340 350 360 GSAGCGIAEA IIAQMVSEGI SDEKARSQVY MVDRWGLLQE GMPNLLDFQQ RLVQKHSNTK 370 380 390 400 410 420 EWENEGNGFS LLDVMRNAKP TVLIGVSGAP GLFSQEVIEE MHKHCKRPIV FPLSNPTSRV 430 440 450 460 470 480 EATPNDIIRW TNGEALVATG SPFDPVVHEG RTYPIAQCNN SYIFPGIGLG VLAVNAKRVT 490 500 510 520 530 540 DEMLMESSRA LATCSPLAIN GRGALLPPLE EIHLVSKKIA FAVAKKAIEQ GVALEITDEA 550 560 LNDAIDQAFW QPVYRRYKRT AF

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000031 Genomic DNA. Translation: BAC59521.1.
RefSeq	NP_797637.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Genome annotation databases
GeneID	1188763.
GenomeReviews	Gene locus VP1258 in contig BA000031_GR.
KEGG	vpa:VP1258.
NMPDR	fig|223926.1.peg.1258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q87Q92.
OMA	Q87Q92. IQDNNET.
Enzyme and pathway databases
BioCyc	VPAR223926:VP1258-MON.
BRENDA	1.1.1.38. 3063.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_VIBPA

Accession

Primary (citable) accession number: Q87Q92

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)