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Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transforms N(2)-succinylglutamate into succinate and glutamate.UniRule annotation

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.UniRule annotation

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase (VP2796)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Zinc1
Metal bindingi66Zinc1
Metal bindingi155Zinc1
Active sitei219UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylaseUniRule annotation (EC:3.5.1.96UniRule annotation)
Gene namesi
Name:astEUniRule annotation
Ordered Locus Names:VP1310
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746511 – 342Succinylglutamate desuccinylaseAdd BLAST342

Interactioni

Protein-protein interaction databases

STRINGi223926.VP1310.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Helixi10 – 15Combined sources6
Beta strandi24 – 27Combined sources4
Beta strandi33 – 38Combined sources6
Beta strandi41 – 45Combined sources5
Beta strandi55 – 60Combined sources6
Helixi68 – 81Combined sources14
Beta strandi89 – 95Combined sources7
Helixi98 – 102Combined sources5
Beta strandi108 – 110Combined sources3
Helixi112 – 114Combined sources3
Beta strandi116 – 119Combined sources4
Helixi125 – 141Combined sources17
Helixi146 – 148Combined sources3
Beta strandi149 – 159Combined sources11
Beta strandi164 – 169Combined sources6
Helixi179 – 188Combined sources10
Beta strandi192 – 195Combined sources4
Helixi203 – 211Combined sources9
Beta strandi214 – 222Combined sources9
Helixi231 – 234Combined sources4
Helixi235 – 245Combined sources11
Beta strandi258 – 267Combined sources10
Beta strandi270 – 277Combined sources8
Beta strandi292 – 296Combined sources5
Beta strandi299 – 302Combined sources4
Beta strandi304 – 307Combined sources4
Beta strandi309 – 312Combined sources4
Beta strandi321 – 329Combined sources9
Beta strandi332 – 335Combined sources4
Beta strandi338 – 341Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BCOX-ray2.33A/B1-342[»]
ProteinModelPortaliQ87Q40.
SMRiQ87Q40.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ87Q40.

Family & Domainsi

Sequence similaritiesi

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiFIIAHPE.

Family and domain databases

CDDicd03855. M14_ASTE. 1 hit.
HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87Q40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSLFRQSF LTDTLDVHID VAPAEQVLSN GVQLKLYQRG VLEVIPENPT
60 70 80 90 100
QETKNIIISC GIHGDETAPM ELVDSIIKDI ESGFQKVDAR CLFIIAHPES
110 120 130 140 150
TLAHTRFLEE NLNRLFDEKE HEPTKELAIA DTLKLLVRDF YQDTEPKTRW
160 170 180 190 200
HLDLHCAIRG SKHYTFAVSP KTRHPVRSKA LVDFLDSAHI EAVLLSNSPS
210 220 230 240 250
STFSWYSAEN YSAQALTMEL GRVARIGENA LDRLTAFDLA LRNLIAEAQP
260 270 280 290 300
EHLSKPCIKY RVSRTIVRLH DDFDFMFDDN VENFTSFVHG EVFGHDGDKP
310 320 330 340
LMAKNDNEAI VFPNRHVAIG QRAALMVCEV KTRFEEGELV YD
Length:342
Mass (Da):38,837
Last modified:June 1, 2003 - v1
Checksum:i4FBA79C89D15D5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC59573.1.
RefSeqiNP_797689.1. NC_004603.1.
WP_005480742.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC59573; BAC59573; BAC59573.
GeneIDi1188815.
KEGGivpa:VP1310.
PATRICi20140830. VBIVibPar50997_1252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC59573.1.
RefSeqiNP_797689.1. NC_004603.1.
WP_005480742.1. NC_004603.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BCOX-ray2.33A/B1-342[»]
ProteinModelPortaliQ87Q40.
SMRiQ87Q40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP1310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC59573; BAC59573; BAC59573.
GeneIDi1188815.
KEGGivpa:VP1310.
PATRICi20140830. VBIVibPar50997_1252.

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiFIIAHPE.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.

Miscellaneous databases

EvolutionaryTraceiQ87Q40.

Family and domain databases

CDDicd03855. M14_ASTE. 1 hit.
HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASTE_VIBPA
AccessioniPrimary (citable) accession number: Q87Q40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.