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Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transforms N(2)-succinylglutamate into succinate and glutamate.UniRule annotation

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.UniRule annotation

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase (VP2796)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Zinc
Metal bindingi66 – 661Zinc
Metal bindingi155 – 1551Zinc
Active sitei219 – 2191UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-1359-MONOMER.
UniPathwayiUPA00185; UER00283.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylaseUniRule annotation (EC:3.5.1.96UniRule annotation)
Gene namesi
Name:astEUniRule annotation
Ordered Locus Names:VP1310
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Succinylglutamate desuccinylasePRO_0000174651Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223926.VP1310.

Structurei

Secondary structure

1
342
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Helixi10 – 156Combined sources
Beta strandi24 – 274Combined sources
Beta strandi33 – 386Combined sources
Beta strandi41 – 455Combined sources
Beta strandi55 – 606Combined sources
Helixi68 – 8114Combined sources
Beta strandi89 – 957Combined sources
Helixi98 – 1025Combined sources
Beta strandi108 – 1103Combined sources
Helixi112 – 1143Combined sources
Beta strandi116 – 1194Combined sources
Helixi125 – 14117Combined sources
Helixi146 – 1483Combined sources
Beta strandi149 – 15911Combined sources
Beta strandi164 – 1696Combined sources
Helixi179 – 18810Combined sources
Beta strandi192 – 1954Combined sources
Helixi203 – 2119Combined sources
Beta strandi214 – 2229Combined sources
Helixi231 – 2344Combined sources
Helixi235 – 24511Combined sources
Beta strandi258 – 26710Combined sources
Beta strandi270 – 2778Combined sources
Beta strandi292 – 2965Combined sources
Beta strandi299 – 3024Combined sources
Beta strandi304 – 3074Combined sources
Beta strandi309 – 3124Combined sources
Beta strandi321 – 3299Combined sources
Beta strandi332 – 3354Combined sources
Beta strandi338 – 3414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BCOX-ray2.33A/B1-342[»]
ProteinModelPortaliQ87Q40.
SMRiQ87Q40. Positions 4-342.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ87Q40.

Family & Domainsi

Sequence similaritiesi

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiFIIAHPE.
OrthoDBiEOG613070.

Family and domain databases

HAMAPiMF_00767. Arg_catab_AstE.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87Q40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSLFRQSF LTDTLDVHID VAPAEQVLSN GVQLKLYQRG VLEVIPENPT
60 70 80 90 100
QETKNIIISC GIHGDETAPM ELVDSIIKDI ESGFQKVDAR CLFIIAHPES
110 120 130 140 150
TLAHTRFLEE NLNRLFDEKE HEPTKELAIA DTLKLLVRDF YQDTEPKTRW
160 170 180 190 200
HLDLHCAIRG SKHYTFAVSP KTRHPVRSKA LVDFLDSAHI EAVLLSNSPS
210 220 230 240 250
STFSWYSAEN YSAQALTMEL GRVARIGENA LDRLTAFDLA LRNLIAEAQP
260 270 280 290 300
EHLSKPCIKY RVSRTIVRLH DDFDFMFDDN VENFTSFVHG EVFGHDGDKP
310 320 330 340
LMAKNDNEAI VFPNRHVAIG QRAALMVCEV KTRFEEGELV YD
Length:342
Mass (Da):38,837
Last modified:June 1, 2003 - v1
Checksum:i4FBA79C89D15D5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC59573.1.
RefSeqiNP_797689.1. NC_004603.1.
WP_005480742.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC59573; BAC59573; BAC59573.
GeneIDi1188815.
KEGGivpa:VP1310.
PATRICi20140830. VBIVibPar50997_1252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC59573.1.
RefSeqiNP_797689.1. NC_004603.1.
WP_005480742.1. NC_004603.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BCOX-ray2.33A/B1-342[»]
ProteinModelPortaliQ87Q40.
SMRiQ87Q40. Positions 4-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP1310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC59573; BAC59573; BAC59573.
GeneIDi1188815.
KEGGivpa:VP1310.
PATRICi20140830. VBIVibPar50997_1252.

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiFIIAHPE.
OrthoDBiEOG613070.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.
BioCyciVPAR223926:GHK4-1359-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ87Q40.

Family and domain databases

HAMAPiMF_00767. Arg_catab_AstE.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.
  2. "X-ray structure of succinylglutamate desuccinalase from Vibrio parahaemolyticus (RIMD 2210633) at the resolution 2.3 A, Northeast structural genomics target Vpr14."
    Northeast structural genomics consortium (NESG)
    Submitted (OCT-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.33 ANGSTROMS) IN COMPLEX WITH ZINC IONS.

Entry informationi

Entry nameiASTE_VIBPA
AccessioniPrimary (citable) accession number: Q87Q40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: November 11, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.