Q87NB7 (RLUB_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal large subunit pseudouridine synthase B
EC=5.4.99.22

Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B

Gene names

Name:	rluB
Ordered Locus Names:	VP1951

Organism

Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) [Complete proteome] [HAMAP]

Taxonomic identifier

223926 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio harveyi group › Vibrio parahaemolyticus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for synthesis of pseudouridine from uracil-2605 in 23S ribosomal RNA By similarity.
Catalytic activity	23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).
Sequence similarities	Belongs to the pseudouridine synthase RsuA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Ribosomal large subunit pseudouridine synthase B		PRO_0000099993
Regions
	Domain	3 – 74	72	S4 RNA-binding
Sites
	Active site	109	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87NB7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6440A74C58CC2893	FASTA	312	35,996
	10 20 30 40 50 60 MSEKLQKVLA RAGHGSRREI ESLIKSGRVS VNGVVAKLGE RLEDESSVVR IDGHIVSAKV 70 80 90 100 110 120 QEEVICRVLA YYKPEGELCT RHDPEGRRTV FDRLPKIRGS RWISVGRLDA NTSGLLLFTT 130 140 150 160 170 180 DGELANRLMH PSRQVEREYL VRVFGEVTEQ KVRNLVKGVE LEDGLARFED VVYAGGEGMN 190 200 210 220 230 240 HTFYVVINEG RNREVRRLWE SQECTVSRLK RVRYGDIFLD KKLPRGGWME LDLKEVNYLR 250 260 270 280 290 300 ELVELRPEKE TMLDLSKDNT SRKRERARSQ KIRRAVKRHE ERVSTSKGRS NNPARRKPKK 310 NAGEQGARNK HR

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC60214.1.
RefSeq	NP_798330.1. NC_004603.1.
3D structure databases
ProteinModelPortal	Q87NB7.
ModBase	Search...
Protein-protein interaction databases
STRING	223926.VP1951.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC60214; BAC60214; BAC60214.
GeneID	1189462.
KEGG	vpa:VP1951.
PATRIC	20142070. VBIVibPar50997_1866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1187.
HOGENOM	HOG000044954.
KO	K06178.
OMA	PRGGWIE.
ProtClustDB	CLSK874246.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR000748. PsdUridine_synth_RsuA/RluB/E/F. IPR018496. PsdUridine_synth_RsuA/RluB_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00093. TIGR00093. 1 hit.
PROSITE	PS01149. PSI_RSU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLUB_VIBPA

Accession

Primary (citable) accession number: Q87NB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program