Q87N39 (DNAE2_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	VP2036

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	1024 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1024	1024	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103401

Sequences

	Sequence		Length	Mass (Da)
	Q87N39 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A75A5C55B48665DA	FASTA	1,024	117,105
	10 20 30 40 50 60 MSYAELFCQS NFSFLTGASH AEELVLQAAF YRYHAIAITD ECSVAGVVKA HATIEQHKLD 70 80 90 100 110 120 IKQIVGSMFW LNEECQIVLL CPCRKAYAEM CRIITNARRR SEKGSYQLSE WDLMSIRHCL 130 140 150 160 170 180 VLWLPTHQAS DHYWGRWLNQ HHNNRLWVAI QRHLGGDDDA YTNHCEKLAH ELQQPITACG 190 200 210 220 230 240 GVLMHTAERL PLQHILTAIK HGCSVDQLGF ERLSNAERAL RPLNKLVRIY KPEWLEESKY 250 260 270 280 290 300 IADLCEFKLS DLKYEYPTEL IPNGYTPNSY LRMLVEQGKE RRFPEGVPED INQTIENELR 310 320 330 340 350 360 LIEDLKYHYY FLTIHDIVMF AKQQGILYQG RGSAANSVVC YCLEITAVDP RQISVLFERF 370 380 390 400 410 420 ISKERKEPPD IDVDFEHERR EEVIQYIYKK YGRERAALAA TVISYRFKSA VREVGKALGI 430 440 450 460 470 480 EETQLDFFIK NVNRRDRSQG WQAQIIELGL QPESLKGQQF IQLVNEIIGF PRHLSQHVGG 490 500 510 520 530 540 FVISSGPLYE LVPVENAAME DRTIIQWDKD DLESLELLKV DVLALGMLNA IRKCFQLIEK 550 560 570 580 590 600 HHQRSLSIAE ITRRQDDPHV YRMLQKADTV GVFQIESRAQ MSMLPRLKPA CYYDLVIQIA 610 620 630 640 650 660 IVRPGPIQGD MVHPFLKRRN GEEPVSYPSE AVKSVLERTM GVPIFQEQVI KLAMVAAGFS 670 680 690 700 710 720 GGEADQLRRA MASWKKNGDL AKFKPKLLNG MQERGYDLAF AERIFEQICG FGEYGFPESH 730 740 750 760 770 780 SASFAVLAYC SAWLKYYYPA EFYTALLNSQ PMGFYSPSQL VQDARRHGVE VLPICVNHSY 790 800 810 820 830 840 YQHHLIQRPN GRLGVQLGFR LVKGFNEEGA TRLVERRPKT GYHSIQEVKQ ILRSRRDIEL 850 860 870 880 890 900 LASANAFQIL SGNRYNARWA AMDSLSDLPL FHHIEEPSVG YQVQPSEYES LIEDYASTGL 910 920 930 940 950 960 SLNRHPITLL EEAGILPRFT RMKQLVDKEH KSLVTVAGVV TGRQSPGTAA GVTFFTLEDD 970 980 990 1000 1010 1020 TGNINVVVWS ATARAQKQAY LTSKILMVKG ILEREGEVIH VIAGKLIDCT HYLSNLQSKS RDFH

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC60299.1.
RefSeq	NP_798415.1. NC_004603.1.
3D structure databases
ProteinModelPortal	Q87N39.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1189547.
GenomeReviews	Gene locus VP2036 in contig BA000031_GR.
KEGG	vpa:VP2036.
NMPDR	fig|223926.1.peg.2036.
PATRIC	20142232. VBIVibPar50997_1947.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	LATNRNG.
ProtClustDB	CLSK910067.
Enzyme and pathway databases
BioCyc	VPAR223926:VP2036-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_VIBPA

Accession

Primary (citable) accession number: Q87N39

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program