Reviewed, UniProtKB/Swiss-Prot Q87MK5 (CHEB1_VIBPA)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
    EC=3.1.1.61

Gene names

Name:	cheB
Ordered Locus Names:	VP2228

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158038
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	176 – 369	194	CheB-type methylesterase
Sites
	Active site	188	1	By similarity
	Active site	215	1	By similarity
	Active site	311	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity
Experimental info
	Sequence conflict	149	1	S → A in AAF32418. Ref.1
	Sequence conflict	170	1	T → A in AAF32418. Ref.1
	Sequence conflict	363	1	L → M in AAF32418. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q87MK5-1 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: 13F7DA7144FE1084	FASTA	369	39,427
	10 20 30 40 50 60 MAIKVLVVDD SSFFRRRVSE IINSESRLEV IDVAVNGREA VEKAKALKPD VITMDIEMPV 70 80 90 100 110 120 MDGITAVREI MAASPTPILM FSSLTHDGAK ATLDALDAGA LDFLPKKFED IARNRDEAVS 130 140 150 160 170 180 LLQQRVIQIA SKRAFMRRPV ARPAAATSSA RPLASRTAAP AASAPARPAT TKFRASGKKY 190 200 210 220 230 240 QLTAIGTSTG GPVALQKILT RLPMNYPHPI VLIQHMPATF TAAFASRLNT LCKIQVKEAQ 250 260 270 280 290 300 DGDVLQAGVA YLAPGGKQMM IDGRAGAARL RIIDGGDRMN YKPCVDVTFG SAAKVYGDKV 310 320 330 340 350 360 LSMVLTGMGA DGREGARMLK SAGSTIWAQD EESCVVYGMP QAVAKAGISS EDLPLDRIAE RMLVEVGLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Components of the polar flagellar switch complex and assembly apparatus." Jaques S., Kim Y.K., McCarter L.L. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BB22.
[2]	"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF069392 Genomic DNA. Translation: AAF32418.1. BA000031 Genomic DNA. Translation: BAC60491.1.
RefSeq	NP_798607.1.
3D structure databases
HSSP	HSSP built from PDB template 1TMY based on UniProtKB Q56312.
ModBase	Search...
Genome annotation databases
GeneID	1189741.
GenomeReviews	Gene locus VP2228 in contig BA000031_GR.
KEGG	vpa:VP2228.
NMPDR	fig|223926.1.peg.2228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q87MK5.
OMA	WAQDEAS.
Enzyme and pathway databases
BioCyc	VPAR223926:VP2228-MON.
BRENDA	3.1.1.61. 3063.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_VIBPA

Accession

Primary (citable) accession number: Q87MK5
Secondary accession number(s): Q9LB11

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)