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Q87MD6

- GLND_VIBPA

UniProt

Q87MD6 - GLND_VIBPA

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-2377-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:VP2320
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002493: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192773Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223926.VP2320.

Structurei

3D structure databases

ProteinModelPortaliQ87MD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 584133HDUniRule annotationAdd
BLAST
Domaini693 – 77785ACT 1UniRule annotationAdd
BLAST
Domaini800 – 87475ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseAdd
BLAST
Regioni333 – 692360Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q87MD6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLQSPLTFS DEQINIGELK QELEKFSSTQ KQEFLNHHPV TSLVLARAEY
60 70 80 90 100
MDLLLTRLWQ YFGFNDIYNI SLVAVGGYGR GELHPLSDID ILVLSNNKLP
110 120 130 140 150
TALEAKISEF ITLLWDLKLE VGHAVRTVNE CAQIGRDDLT VATNLQEARL
160 170 180 190 200
LCGSEDTFQA LKKVVLSDSF WPSETFYRAK IQEQRERHAR YHDTTYNLEP
210 220 230 240 250
DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSRYGFLTDA EYRELVECQD
260 270 280 290 300
FLWRVRFALH IELRRYDNRL TFAHQAQVAE NLGYVGEGNR GVEMMMKEFY
310 320 330 340 350
RTLRRVAELN KMLLKLFDQA IINGGATENA EILDADFQRR GSLIEARKPA
360 370 380 390 400
LFQARPETIL DMFLHIANDS TIEGVSPPTL RQLRTARRRL NKFLHTIPAA
410 420 430 440 450
REKFLALCRH PNALHKAFSL MHRLGVMAAY LPQWSQIVGQ MQFDLFHAYT
460 470 480 490 500
VDEHSIRLLK HINTFNNPDN HAKHPICCDI YPRMQKKELL IIAAIFHDIG
510 520 530 540 550
KGRGGDHSVI GEGEAYDFCI EHGLSKPEAK LVGWLVRHHL LMSVTAQRRD
560 570 580 590 600
IYDPDVITEF AKQVRDEESL EYLVCLTVAD ICATNPELWN AWKRTLLAEL
610 620 630 640 650
FYSTQRALRR GLENPVDVRE RIRHNQQMAS ALLRKEGFSA RQIEVLWQRF
660 670 680 690 700
KADYFLRHTH TQIAWHCAHL LRMDDPNKPL VLISKKATRG GTEVFVYTKD
710 720 730 740 750
QPALFATVVA ELDRRNFNVH DAQIMTSKDG HVIDTFMVLD QHGEAIDESR
760 770 780 790 800
HAAVIKHLTH VLEAGRPTKI KTRRTPNKLQ HFNVKTKVDF LPTKGKKHTL
810 820 830 840 850
MEFVALDTPG LLAKVGRTFA DLNINLHGAK ITTIGERAED LFILTSEAGG
860 870
RLSEEQQNEL RDKLIEKLSD AVTA
Length:874
Mass (Da):100,247
Last modified:June 1, 2003 - v1
Checksum:i1B849BC63393E97E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC60583.1.
RefSeqiNP_798699.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC60583; BAC60583; BAC60583.
GeneIDi1189833.
KEGGivpa:VP2320.
PATRICi20142786. VBIVibPar50997_2222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC60583.1 .
RefSeqi NP_798699.1. NC_004603.1.

3D structure databases

ProteinModelPortali Q87MD6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 223926.VP2320.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC60583 ; BAC60583 ; BAC60583 .
GeneIDi 1189833.
KEGGi vpa:VP2320.
PATRICi 20142786. VBIVibPar50997_2222.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci VPAR223926:GHK4-2377-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.

Entry informationi

Entry nameiGLND_VIBPA
AccessioniPrimary (citable) accession number: Q87MD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3