Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi487MagnesiumUniRule annotation1
Metal bindingi493MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciVPAR223926:G1GTB-2508-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:VP2452
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003299311 – 711Polyribonucleotide nucleotidyltransferaseAdd BLAST711

Proteomic databases

PRIDEiQ87M06

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi223926.VP2452

Structurei

3D structure databases

ProteinModelPortaliQ87M06
SMRiQ87M06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 613KHUniRule annotationAdd BLAST60
Domaini623 – 691S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218326
KOiK00962
OMAiRYMHNYN

Family and domain databases

Gene3Di3.30.1370.10, 1 hit
3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF03725 RNase_PH_C, 1 hit
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Q87M06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEKPVVKTF QYGNHTVTLE TGVIARQATA AVMVTMDDTA VFVSVVGKKE
60 70 80 90 100
AVAGQDFFPL TVNYQERTYA AGKIPGGFFK REGRPSEGET LTARLIDRPI
110 120 130 140 150
RPLFPDAFKN EVQVIATVVS VNPDVQPDIP TMIGTSAALA ISGIPFNGPI
160 170 180 190 200
GAARVGHIDG QLVLNPSQTE LNASRLDLVV AGTESAVLMV ESEADNLTEE
210 220 230 240 250
EMLAAVVFGH DQQQVVINAI NEFKAEVATP AWDWVAPEEN TALKTKIAEL
260 270 280 290 300
AEAKLVEAYQ ITEKMARYDR IHEIAAEVNE ALLAQDPEAD TKEIHTIFHD
310 320 330 340 350
LEKTVVRRSI IAGNPRIDGR EKDMVRALDV RTGVLPRTHG SALFTRGETQ
360 370 380 390 400
ALVTATLGTQ RDAQIIDELT GERKDHFLLH YNFPPYCVGE TGFVGSPKRR
410 420 430 440 450
EIGHGKLAKR GIAAVMPSVD EFPYTVRVVS EITESNGSSS MASVCGTSLA
460 470 480 490 500
LMDAGVPIKA SVAGIAMGLV KEGDDFVVLS DILGDEDHLG DMDFKVAGTS
510 520 530 540 550
TGVTALQMDI KIEGITKEIM QIALNQAQGA RKHILSVMDE ALAGARDDIS
560 570 580 590 600
EFAPRIHTMK ISAEKIKDVI GKGGAVIRAL TEETGTTIEI EDDGTIKIAA
610 620 630 640 650
TEGAAAKEAI RRIEEITAEV EVGRIYTGKV ARLADFGAFV TVLPGKDGLV
660 670 680 690 700
HISQIAEKRV EKVSDYLTEG QEVQVKVLEI DRQGRVRLSM KEAVEKPAEE
710
AAAEAPAAKE E
Length:711
Mass (Da):76,738
Last modified:June 1, 2003 - v1
Checksum:iBBF41F227161F563
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA Translation: BAC60715.1
RefSeqiNP_798831.1, NC_004603.1
WP_005456067.1, NC_004603.1

Genome annotation databases

EnsemblBacteriaiBAC60715; BAC60715; BAC60715
GeneIDi1189965
KEGGivpa:VP2452
PATRICifig|223926.6.peg.2353

Entry informationi

Entry nameiPNP_VIBPA
AccessioniPrimary (citable) accession number: Q87M06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health