Q87LQ2 (ISPD1_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
MEP cytidylyltransferase 1
Short name=MCT 1

Gene names

Name:	ispD1
Synonyms:	ispD
Ordered Locus Names:	VP2559

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108		PRO_0000075644
Sites
	Site	20	1	Transition state stabilizer By similarity
	Site	27	1	Transition state stabilizer By similarity
	Site	159	1	Positions MEP for the nucleophilic attack By similarity
	Site	215	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87LQ2 [UniParc]. Last modified May 23, 2003. Version 1. Checksum: E92046C6DE8C0682	FASTA	234	25,470
	10 20 30 40 50 60 MSENVSSHIA IVPAAGVGSR MKADRPKQYL LIDGKTVLEH TVEKLLAHPQ IAKVVVAVTE 70 80 90 100 110 120 GDPYYPELSI ALHPDVIRVA GGKERADSVL SGLNYVSAQL PCEWVLVHDA ARPCVTLNDI 130 140 150 160 170 180 DRLIDVCCAH PTGGILASPV RDTMKRANKE NNIDHTVDRE ALWHALTPQM FKTQQLTRAL 190 200 210 220 230 ADALQQGVAI TDEASALEWL GETPALVQGS ANNIKITQPE DLALAEFYLS RERG

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC60822.1.
RefSeq	NP_798938.1. NC_004603.1.
3D structure databases
ProteinModelPortal	Q87LQ2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1190083.
GenomeReviews	Gene locus VP2559 in contig BA000031_GR.
KEGG	vpa:VP2559.
NMPDR	fig|223926.1.peg.2559.
PATRIC	20143278. VBIVibPar50997_2457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	VPAR223926:VP2559-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD1_VIBPA

Accession

Primary (citable) accession number: Q87LQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	May 23, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program