Reviewed, UniProtKB/Swiss-Prot Q87LL0 (E4PD_VIBPA)

Names and origin

Protein names

Recommended name:
    D-erythrose-4-phosphate dehydrogenase
      Short name=E4PDH
    EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	VP2601

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity.
Catalytic activity	D-erythrose 4-phosphate + NAD(+) + H(2)O = 4-phosphoerythronate + NADH.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	D-erythrose-4-phosphate dehydrogenase		PRO_0000293175
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	158 – 160	3	Substrate binding Potential
	Region	217 – 218	2	Substrate binding Potential
Sites
	Active site	159	1	Nucleophile By similarity
	Binding site	204	1	Substrate Potential
	Binding site	240	1	Substrate Potential
	Binding site	322	1	NAD By similarity
	Site	186	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87LL0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 75AA5F1B623DCEE5	FASTA	345	38,249
	10 20 30 40 50 60 MLKVAINGFG RIGRNVLRAV YESGKHQQIK VVAVNELAQP EAMAHLLQYD TSHGRFGKKI 70 80 90 100 110 120 SHDQEHLNVH HESGEYDAIR ILHLSEIELL PWRDLEVDIV LDCTGVYGSK ADGLAHIEAG 130 140 150 160 170 180 AKKVLFSHPG ANDLDNTIIY GVNHETLKDE HRVVSNGSCT TNCIVPIIKV LDEAFGIESG 190 200 210 220 230 240 TITTIHSSMN DQQVIDAYHN DLRRTRAASQ SIIPVDTKLH KGIERIFPKF SNKFEAISVR 250 260 270 280 290 300 VPTVNVTAMD LSVTINTNVK VNDVNQTIVN ASQCTLRNIV DYTESPLVSI DFNHDPHSAI 310 320 330 340 VDGTQTRVSN GQLVKMLVWC DNEWGFANRM LDTALAMKAS SQVEL

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000031 Genomic DNA. Translation: BAC60864.1.
RefSeq	NP_798980.1.
3D structure databases
HSSP	HSSP built from PDB template 1GD1 based on UniProtKB P00362.
ModBase	Search...
Genome annotation databases
GeneID	1190125.
GenomeReviews	Gene locus VP2601 in contig BA000031_GR.
KEGG	vpa:VP2601.
NMPDR	fig|223926.1.peg.2601.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q87LL0.
Enzyme and pathway databases
BioCyc	VPAR223926:VP2601-MON.
Family and domain databases
HAMAP	MF_01640. [Tree]
InterPro	IPR006422. E4P_DHase_bac. IPR000173. GlycerAld_3-P_DHase. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit.
PROSITE	PS00071. GAPDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_VIBPA

Accession

Primary (citable) accession number: Q87LL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)