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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei93S-adenosyl-L-methionineUniRule annotation1
Binding sitei120S-adenosyl-L-methionineUniRule annotation1
Active sitei143By similarity1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciVPAR223926:G1GTB-2692-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:VP2625
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714191 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Proteomic databases

PRIDEiQ87LI7

Interactioni

Protein-protein interaction databases

STRINGi223926.VP2625

Structurei

3D structure databases

ProteinModelPortaliQ87LI7
SMRiQ87LI7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 220Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q87LI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVTTNEYN EDGKLIRKIR SFVRREGRLT KGQENAMNEC WPTMGIDYKA
60 70 80 90 100
ELLDWKEVFG NDNPVVLEIG FGMGASLVEM AKNAPEKNFF GIEVHSPGVG
110 120 130 140 150
ACLSDAREAG ITNLRVMCHD AVEVFEHMIP NDSLATLQLF FPDPWHKKRH
160 170 180 190 200
HKRRIVQLEF AEMVRQKLIP NEGIFHMATD WENYAEHMIE IMNQAPGFEN
210 220 230
IAQDGDFVPR PEDRPLTKFE ARGHRLGHGV WDIKYKRIA
Length:239
Mass (Da):27,507
Last modified:June 16, 2003 - v1
Checksum:i9001F26E5A542915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA Translation: BAC60888.1
RefSeqiNP_799004.1, NC_004603.1
WP_005482471.1, NC_004603.1

Genome annotation databases

EnsemblBacteriaiBAC60888; BAC60888; BAC60888
GeneIDi1190149
KEGGivpa:VP2625
PATRICifig|223926.6.peg.2521

Similar proteinsi

Entry informationi

Entry nameiTRMB_VIBPA
AccessioniPrimary (citable) accession number: Q87LI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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