Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

Gene

gppA

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.UniRule annotation

Catalytic activityi

Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate.UniRule annotation

Pathway: ppGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-3139-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphataseUniRule annotation (EC:3.6.1.40UniRule annotation)
Alternative name(s):
Guanosine pentaphosphate phosphohydrolaseUniRule annotation
pppGpp-5'-phosphohydrolaseUniRule annotation
Gene namesi
Name:gppAUniRule annotation
Ordered Locus Names:VP3003
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatasePRO_0000194294Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223926.VP3003.

Structurei

3D structure databases

ProteinModelPortaliQ87KH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family. GppA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiELANECW.
OrthoDBiEOG6M6JN6.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87KH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQAGSSPLY AAIDLGSNSF HMLVVRHIDG SVQTMAKIKR KVRLAAGLDE
60 70 80 90 100
HNSLSMEAMQ RGWDCLSLFA ERLQDIPTQN IRIVGTATLR TATNVDVFLE
110 120 130 140 150
KANQILGQPI EVISGEEEAA TIYKGVAHTS GGSGRRLVVD IGGASTELII
160 170 180 190 200
GEGFEAKALT SLKMGCVTWL ENFFKDRQLN ARNFEAAIEG AKQTIKPILE
210 220 230 240 250
QYTDLGWDVC VGASGTVQAL QEIMLAQGMD EVITHSKLKR LQKQAMLADH
260 270 280 290 300
LEELDIEGLT LERALVFPSG LSILIAIFEL LEIDAMTLAG GALREGLVYE
310 320 330 340 350
MVDELRQNDI RARTICSVQS RYQLDCQYGE QVATLAGKLL EQAGGDEWIA
360 370 380 390 400
EPQGKVLLET TAKLHEIGLT IDFKKGGEHS AYLLQNLDLP GYTRAQKFFI
410 420 430 440 450
GEIARRYREQ LSSLPEQHAI SGTSAKRVLR LLRLAVLLTH RRNPSLEPQV
460 470 480 490
ELLAEGDKLT LSIDAKWLEA NPLTAAELEI ESNRQTDIGW PLTITAC
Length:497
Mass (Da):54,689
Last modified:June 1, 2003 - v1
Checksum:i28977E25011DD2CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC61266.1.
RefSeqiNP_799382.1. NC_004603.1.
WP_005480964.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC61266; BAC61266; BAC61266.
GeneIDi1190595.
KEGGivpa:VP3003.
PATRICi20144276. VBIVibPar50997_2888.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC61266.1.
RefSeqiNP_799382.1. NC_004603.1.
WP_005480964.1. NC_004603.1.

3D structure databases

ProteinModelPortaliQ87KH4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP3003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC61266; BAC61266; BAC61266.
GeneIDi1190595.
KEGGivpa:VP3003.
PATRICi20144276. VBIVibPar50997_2888.

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiELANECW.
OrthoDBiEOG6M6JN6.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciVPAR223926:GHK4-3139-MONOMER.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.

Entry informationi

Entry nameiGPPA_VIBPA
AccessioniPrimary (citable) accession number: Q87KH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.