Q87KE0 (PURK_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase

Gene names

Name:	purK
Ordered Locus Names:	VP3037

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.
Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide synthase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	N5-carboxyaminoimidazole ribonucleotide synthase		PRO_0000075017
Regions
	Domain	97 – 287	191	ATP-grasp
	Nucleotide binding	175 – 178	4	ATP By similarity
	Nucleotide binding	257 – 258	2	ATP By similarity
Sites
	Binding site	93	1	ATP By similarity
	Binding site	133	1	ATP By similarity
	Binding site	144	1	ATP By similarity
	Binding site	183	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87KE0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 977C1551533246C4	FASTA	377	41,049
	10 20 30 40 50 60 MHVLVLGSGQ LARMMSLAGA PLNIQISAYD VGSGNVVHPL TQQVLGHGLE NAIEQVDAIT 70 80 90 100 110 120 AEFEHIPHDV LDICELSGKF LPSTAAIKAG GDRRVEKALL DNAGVRNAKH YVIETREDFE 130 140 150 160 170 180 RAIEHVGIPM VLKSALGGYD GKGQWRLKEA AQIETIWAEM AECIAATPTQ AIVAEEFVPF 190 200 210 220 230 240 NREVSLVGAR GKDGSVEVYP LAENVHTNGV LSLSTAIDAP ELQAQAKQMF TAVADSLNYV 250 260 270 280 290 300 GVLALEFFDV EGTLLVNEIA PRVHNSGHWT QQGAETCQFE NHLRAVCGLP LGSTKLIRET 310 320 330 340 350 360 SMVNILGEDT LPEALLAMDG CHIHWYGKEK REGRKMGHIN VCGDYPGELH RRLCALAEVL 370 DPMIFPAVHE FAKQAQR

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000031 Genomic DNA. Translation: BAC61300.1.
RefSeq	NP_799416.1. NC_004603.1.
3D structure databases
ProteinModelPortal	Q87KE0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1190636.
GenomeReviews	Gene locus VP3037 in contig BA000031_GR.
KEGG	vpa:VP3037.
NMPDR	fig|223926.1.peg.3037.
PATRIC	20144356. VBIVibPar50997_2921.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG516369.
OMA	GRKMGHF.
ProtClustDB	PRK06019.
Enzyme and pathway databases
BioCyc	VPAR223926:VP3037-MONOMER.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01589.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_VIBPA

Accession

Primary (citable) accession number: Q87KE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program