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Protein

Pyrimidine/purine nucleoside phosphorylase

Gene

ppnP

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. Also catalyzes the reverse reactions.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation
Adenosine + phosphate = adenine + alpha-D-ribose 1-phosphate.UniRule annotation
Cytidine + phosphate = cytosine + alpha-D-ribose 1-phosphate.UniRule annotation
Guanosine + phosphate = guanine + alpha-D-ribose 1-phosphate.UniRule annotation
Inosine + phosphate = hypoxanthine + alpha-D-ribose 1-phosphate.UniRule annotation
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.UniRule annotation
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate.UniRule annotation
Xanthosine + phosphate = xanthine + alpha-D-ribose 1-phosphate.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-3279-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidine/purine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.2UniRule annotation)
Alternative name(s):
Adenosine phosphorylaseUniRule annotation
Cytidine phosphorylaseUniRule annotation
Guanosine phosphorylaseUniRule annotation (EC:2.4.2.15UniRule annotation)
Inosine phosphorylaseUniRule annotation
Thymidine phosphorylaseUniRule annotation (EC:2.4.2.4UniRule annotation)
Uridine phosphorylaseUniRule annotation (EC:2.4.2.3UniRule annotation)
Xanthosine phosphorylaseUniRule annotation
Gene namesi
Name:ppnPUniRule annotation
Ordered Locus Names:VPA0057
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002117861 – 94Pyrimidine/purine nucleoside phosphorylaseAdd BLAST94

Interactioni

Protein-protein interaction databases

STRINGi223926.VPA0057.

Structurei

Secondary structure

194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi9 – 11Combined sources3
Beta strandi13 – 20Combined sources8
Beta strandi23 – 30Combined sources8
Beta strandi32 – 41Combined sources10
Beta strandi43 – 56Combined sources14
Beta strandi63 – 66Combined sources4
Beta strandi70 – 73Combined sources4
Beta strandi75 – 85Combined sources11
Beta strandi87 – 92Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OYZX-ray1.71A2-94[»]
ProteinModelPortaliQ87K41.
SMRiQ87K41.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ87K41.

Family & Domainsi

Sequence similaritiesi

Belongs to the nucleoside phosphorylase PpnP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NBK. Bacteria.
COG3123. LUCA.
HOGENOMiHOG000218057.
KOiK09913.
OMAiYHYICHF.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01537. Nucleos_phosphorylase_PpnP. 1 hit.
InterProiView protein in InterPro
IPR009664. Ppnp.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
PfamiView protein in Pfam
PF06865. DUF1255. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q87K41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIKENSYFA GGVKSLGFNQ HGQDVSVGVM LPGEYTFGTQ APERMTVVKG
60 70 80 90
ALVVKRVGEA DWTTYSSGES FDVEGNSSFE LQVKDATAYL CEYL
Length:94
Mass (Da):10,212
Last modified:June 1, 2003 - v1
Checksum:i5A200505EB8DA0E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000032 Genomic DNA. Translation: BAC61400.1.
RefSeqiNP_799567.1. NC_004605.1.
WP_005464991.1. NC_004605.1.

Genome annotation databases

EnsemblBacteriaiBAC61400; BAC61400; BAC61400.
GeneIDi1190736.
KEGGivpa:VPA0057.
PATRICifig|223926.6.peg.3017.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPPNP_VIBPA
AccessioniPrimary (citable) accession number: Q87K41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families