Reviewed, UniProtKB/Swiss-Prot Q87J02 (KATG1_VIBPA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	VPA0453

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. HAMAP MF_01961
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354950
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87J02-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5BA3D0C7DFF33925	FASTA	721	80,486
	10 20 30 40 50 60 MTQQNAHSEG KCPVMHGSMT TNNRTEKNWW PKSLNLDILH QHDAKTNPMP SDFDYQEEVK 70 80 90 100 110 120 KLDFSALKQD LIALMTDSQE WWPADWGHYG GLMIRMSWHA AGTYRIADGR GGAGTGNLRF 130 140 150 160 170 180 APLNSWPDNA NLDKARRILW PIKKKYGNQL SWADLIAYAG TMAYESMGLK TFGFGFGRED 190 200 210 220 230 240 IWHPEKDIYW GSEKEWLAPT NNPNSRYSGE RDLENPLAAV MMGLIYVNPE GVDGQPDPLK 250 260 270 280 290 300 TAHDVRVTFA RMAMNDEETV ALTAGGHTVG KAHGNGDAAN LGPEPEGADI HDQGLGWLNK 310 320 330 340 350 360 TTRGVGNNAV TSGIEGAWTS QPTQWDNGYF HLLLNYDWEL KKSPAGAWQW EPIDIKEEDK 370 380 390 400 410 420 PVDPENPNVR HNPIMTDADM AMKMDPEYRK ISERFHSDPA YFADTFARAW FKLTHRDMGP 430 440 450 460 470 480 KARYIGPDVP QEDLIWQDPV PNGNANYDID AVKAKIAASG LSVSDMVTTA WDSARTFRQS 490 500 510 520 530 540 DKRGGANGAR VRLAPQKDWQ GNEPERLARV LPVLENIAKD TGASVADVVV LAGNVGIEQA 550 560 570 580 590 600 ASAAGVNVTV PFLPGRGDAT QEMTDVESFE VLEPLHDGYR NWLKQNYVVT PEEMLLDRTQ 610 620 630 640 650 660 LMGLTAAEMT VLVGGMRVLG TNHGGSKHGV FTDRVGQLTN DFFINLTDMK YTWEPVGENL 670 680 690 700 710 720 YEIRSRRSKD VKWTATRVDL VFGSNSILRA YAELYAQDDN AGKFVEDFVA AWTKVMNADR F

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000032 Genomic DNA. Translation: BAC61796.1.
RefSeq	NP_799963.1.
3D structure databases
HSSP	HSSP built from PDB template 1U2L based on UniProtKB P13029.
SMR	Q87J02. Positions 29-721.
ModBase	Search...
Protein family/group databases
PeroxiBase	2655. VpCP01_RIMD2210633.
Genome annotation databases
GeneID	1191141.
GenomeReviews	Gene locus VPA0453 in contig BA000032_GR.
KEGG	vpa:VPA0453.
NMPDR	fig|223926.1.peg.3533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	NGWANSV.
Enzyme and pathway databases
BioCyc	VPAR223926:VPA0453-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_VIBPA

Accession

Primary (citable) accession number: Q87J02

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)