Q87I38 (KATG2_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 2
Short name=CP 2
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	VPA0768

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354951
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87I38 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6F552DAA3ECD9EA9	FASTA	724	80,704
	10 20 30 40 50 60 MSNTNGGSVG KCPVMHGGQT STDKSVMDWW PNALNLDILH QHDSKTNPFG PDFNYKEELK 70 80 90 100 110 120 KLDVDALKQD LKDLMTNSQV WWPADWGHYG GLMIRMAWHA AGSYRIADGR GGAATGNQRF 130 140 150 160 170 180 APLNSWPDNA NLDKARRLLW PIKRKYGNKL SWADLIILAG NMAYESMGFK TFGFGFGRED 190 200 210 220 230 240 IWHPEKDTYW GSEQEWLAPS GAKNSRYSGE RDLENPLAAV MMGLIYVNPE GVDGNPDPLK 250 260 270 280 290 300 TAQDMRVTFA RMAMNDEETV ALTAGGHTVG KCHGNGDAAN LGPDPEGADV HEQGLGWMNH 310 320 330 340 350 360 KTRGIGRDTV TSGLEGAWTT HPTQWDNGYF YLLFKYDWEL KKSPAGAWQW EPIDIEEQDK 370 380 390 400 410 420 PVDVEDGSKR YNPIMTDADM ALKMDPEYRK ISERFQQDPA YFEDMFARAW FKLTHRDMGP 430 440 450 460 470 480 KSCYLGPDVP SEDLIWQDPT PAGKTDYNVD LVKGKIEASG LSIADLVATA WDSARTYRGS 490 500 510 520 530 540 DRRGGANGAR IRLAPQKDWQ GNEPERLSRV LAVLESIAAE EGCSVADAIV LAGNVGIELA 550 560 570 580 590 600 ARAAGHDVSV PFAPGRGDAS QDMTDVESFE VLEPVADGFR NWLKKDYVVK PEELLLDRAQ 610 620 630 640 650 660 LMGLTAPEMT VLIGGLRVLG SNYGGSKDGV FTDRVGTLSN DFFVNLTDMA YTWKPAGENQ 670 680 690 700 710 720 YEIRDRKTDQ VKWTATRVDL VFGSNSILRS YAEVYAQDDN QEKFIHDFVA AWTKVMNADR FDLQ

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000032 Genomic DNA. Translation: BAC62111.1.
RefSeq	NP_800278.1. NC_004605.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q87I38.
ModBase	Search...
Protein family/group databases
PeroxiBase	2656. VpCP02_RIMD2210633.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1191457.
GenomeReviews	Gene locus VPA0768 in contig BA000032_GR.
KEGG	vpa:VPA0768.
NMPDR	fig|223926.1.peg.3848.
PATRIC	20145937. VBIVibPar50997_3701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	VPAR223926:VPA0768-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_VIBPA

Accession

Primary (citable) accession number: Q87I38

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program