Reviewed, UniProtKB/Swiss-Prot Q87GW6 (NAPA_VIBPA)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	VPA1199

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 829	799	Periplasmic nitrate reductase		PRO_0000046011
Sites
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	83	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87GW6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9E42579656BA0C13	FASTA	829	92,961
	10 20 30 40 50 60 MKMTRRAFVK ANAAASAAAV AGITLPASAA NLIASSDQTK ITWDKAPCRF CGTGCSVLVG 70 80 90 100 110 120 TQNGKVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGQDRLT QPLLRMKDGK YHKDGEFTPV 130 140 150 160 170 180 SWDVAFDTMA EKWKASLEKK GPTSVGMFGS GQWTVMEGYA AAKMMKAGFR SNNIDPNARH 190 200 210 220 230 240 CMASAVVGFM RAFGIDEPMG CYDDFENADA FVLWGSNMAE MHPVLWTRIT DRRLSHPHVR 250 260 270 280 290 300 VNVLSTYYHR SFELADHGYI FNPQSDLAIA NFIANYIIEN DAVNWDFVNK HTNFTQADTD 310 320 330 340 350 360 IGYGLRDDDP LQKAAKNPNS GKLTSISFEE YKKSVAPYTV EKASEISGVE KEKLIELAKQ 370 380 390 400 410 420 YADPNTKVMS LWTMGMNQHT RGVWMNNLVY NIHLLTGKIA TPGNSPFSLT GQPSACGTAR 430 440 450 460 470 480 EVGTFAHRLP ADMVVANPKH RQIAEKIWKL PEGTIPPKPG FHAVLQDRML NDGVLNCYWV 490 500 510 520 530 540 QCNNNMQAGP NINTERLPGY RNPENFIVVS DPYPTATAQA ADLILPTAMW IEKEGAYGNA 550 560 570 580 590 600 ERRTQAWYQQ VGTVGDAKSD LWQVMEFSKR FKMEEVWPEE LLAKAPQYRG KTMYDMLFKN 610 620 630 640 650 660 GQVDKFPLEE ARELNDDSHH FGFYVQKGLF EEYATFGRGH GHDLAPYDVY HTVRGLRWPV 670 680 690 700 710 720 VDGKETQWRF KEGSDPYAKA GSGWDFYGNA DGKAKIISAP YEAPPEVPDS EFDLWLCTGR 730 740 750 760 770 780 VLEHWHTGTM TRRVPELYKA VPDAVCYMHP EDAKARNVRR GEEVVIANKR GEVRVRVETR 790 800 810 820 GRNRPPKGLV FVPFFDARIL INKLILDATD PLSKQTDFKK CPVKITKVA

References

[1]

"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000032 Genomic DNA. Translation: BAC62542.1.
RefSeq	NP_800709.1.
3D structure databases
HSSP	HSSP built from PDB template 1OGY based on UniProtKB Q53176.
SMR	Q87GW6. Positions 41-827.
ModBase	Search...
Genome annotation databases
GeneID	1191895.
GenomeReviews	Gene locus VPA1199 in contig BA000032_GR.
KEGG	vpa:VPA1199.
NMPDR	fig|223926.1.peg.4279.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q87GW6.
Enzyme and pathway databases
BioCyc	VPAR223926:VPA1199-MON.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_VIBPA

Accession

Primary (citable) accession number: Q87GW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)