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Q87FR0 (GLGB_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:VPA1618
OrganismVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) [Complete proteome] [HAMAP]
Taxonomic identifier223926 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length755 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7557551,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188761

Sites

Active site4351Nucleophile By similarity
Active site4881Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87FR0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8F052368B6B57D79

FASTA75587,504
        10         20         30         40         50         60 
MSSQRWLWRN QHSVQTNIGS REKDLKITKT KKIKKKHERA YELLAEAAYS DPFAALGPFI 

        70         80         90        100        110        120 
DDGEGSLRVW MPGANKVELL VNGEPRVALE RDGDSGFILK EQRDLHLTHY RLAVDWNGVE 

       130        140        150        160        170        180 
QIIDDPYQYH NIYQEYEHLH TPKDMYHYMG AHFVTLERGG ENISGVRFLV YAPHASAVSL 

       190        200        210        220        230        240 
VGCFNQWDGR RHPMQRLDYG IWGLFIPGLE EGVQYKFELK GPNGEGLPHK QDPWGFYSEQ 

       250        260        270        280        290        300 
YPSFASITYD HKRYQWQDAK WQNRAVTQKR DEALSFYELH AGSWKRDGKG DFLNYRELAE 

       310        320        330        340        350        360 
QLVPYLVDMG YTHVELMPVS EHPFYGSWGY QPVGLFAPTS RYGSPDDFKF FVDACHQAGI 

       370        380        390        400        410        420 
GVVLDWVPAH FPSDDHGLAN FDGTPLFHDP DPRRGWHQDW NSYIYDLGRE HVRRFLVSNA 

       430        440        450        460        470        480 
LYWFEQFHID GIRVDAVASM LYLDYSRSHD QWVPNVDGGN ENYDAIATLK WMNEEVYKHF 

       490        500        510        520        530        540 
PNAMTIAEES TAFPGVSAPT FMGGLGFGFK WNMGWMHDSL SYVKEDPVHR KYHHNTITFP 

       550        560        570        580        590        600 
LVYAHSENYV LSLSHDEVVY GKGSIHNKMP GDEWQQTANL RAYYGYMYGQ PGKKLNFMGA 

       610        620        630        640        650        660 
EIGQTAEWNH DDQLQWFLLE YERHQGVQKL MRDLNHLYRN EAAMHDQDCV PAGFEWRLQD 

       670        680        690        700        710        720 
EADASILAHE RISKEGERIL IITNFTPVPH ERFRLGVPNV GQYELLLNTD DSKYGGSDFK 

       730        740        750 
VLTSVKTEKV ESESLPQSLE LRLPPLSTVF YKLHK 

« Hide

References

[1]"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RIMD 2210633.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000032 Genomic DNA. Translation: BAC62961.1.
RefSeqNP_801128.1. NC_004605.1.

3D structure databases

ProteinModelPortalQ87FR0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING223926.VPA1618.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC62961; BAC62961; BAC62961.
GeneID1192314.
KEGGvpa:VPA1618.
PATRIC20147621. VBIVibPar50997_4538.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycVPAR223926:GHK4-4858-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_VIBPA
AccessionPrimary (citable) accession number: Q87FR0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries