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Q87EL0 (PROB_XYLFT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:PD_0296
OrganismXylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]
Taxonomic identifier183190 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109761

Regions

Domain288 – 37083PUA
Nucleotide binding181 – 1822ATP By similarity
Nucleotide binding223 – 2297ATP By similarity

Sites

Binding site241ATP By similarity
Binding site641Substrate By similarity
Binding site1491Substrate By similarity
Binding site1611Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87EL0 [UniParc].

Last modified June 16, 2003. Version 1.
Checksum: 03AE5E0240CA1304

FASTA38440,872
        10         20         30         40         50         60 
MTGIPPPSRF PEQPIPPWRR AVLKVGSSLL AADGGGLSPR FALDLAHFVS ANITAGRQLV 

        70         80         90        100        110        120 
IVSSGAVAAG RALIPPLPES GGALAARQAL AALGQAQLIA LWQRFFDRPV AQVLLTHDDL 

       130        140        150        160        170        180 
RNRRRYLNAR ATLRELLHLG TLPVVNENDT VSVDELKLGD NDNLAAIVAA LIDAQALFIA 

       190        200        210        220        230        240 
TDIDGLYTTD PRHHSDAQPL HEVRTLTPEN LAMAGDSSST VGTGGMRTKL EAALKAGAAG 

       250        260        270        280        290        300 
IDTYLFNGRS SDVVRGLAQH RLRGTRIHPT CTPIAARKYW LRHAPVEPGA ILIDAGAAAA 

       310        320        330        340        350        360 
LAQQGASLLP GGVLSAEGDF RRGDMIQIAT RSPDHPSHPL ARGLVQYSAA DVRRIAGCHS 

       370        380 
RDIQTLLGYT YGDTIVHRDD LVLL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009442 Genomic DNA. Translation: AAO28181.1.
RefSeqNP_778532.1. NC_004556.1.

3D structure databases

ProteinModelPortalQ87EL0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING183190.PD0296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO28181; AAO28181; PD_0296.
GeneID1144508.
KEGGxft:PD0296.
PATRIC24148134. VBIXylFas71109_0392.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
KOK00931.
OMARLMRAYE.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycXFAS183190:GIX4-296-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_XYLFT
AccessionPrimary (citable) accession number: Q87EL0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways