Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q87DZ9 (LIPA_XYLFT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase

EC=2.8.1.8
Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene names
Name:lipA
Ordered Locus Names:PD_0530
OrganismXylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]
Taxonomic identifier183190 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Lipoyl synthase HAMAP-Rule MF_00206
PRO_0000102386

Sites

Metal binding811Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding861Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding921Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1071Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1111Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1141Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87DZ9 [UniParc].

Last modified June 16, 2003. Version 1.
Checksum: C365D761D8C239EF

FASTA37341,126
        10         20         30         40         50         60 
MTQPIIRSIP LHVVSNDHPS SSPLQPGVKQ SGEDKIGRSP VQFADVPVLR KPSWIRVRIP 

        70         80         90        100        110        120 
SGNAVQSLKA KLRENRLVTV CEEAACPNIH ECFSHGTATF MILGEVCTRR CSFCDVAHGR 

       130        140        150        160        170        180 
PKPPDPEEPI SLARTVAEMG LKYVVVTSVD RDDLRDGGAQ HFVDCIAAIR QSAPQTRIEI 

       190        200        210        220        230        240 
LTPDFRGKGR MDRALDILAA CPPDVFNHNV ETVPALYPNV RPGADYQWSL TLLKRFKAQH 

       250        260        270        280        290        300 
PQVPTKSGIM LGLGETLDQV QATLRDLRAH DVDMVTVGQY LQPTPHHHPV LRYWTPDEYK 

       310        320        330        340        350        360 
ALEEYGMALG FSHVASGPMV RSSYHADHQA KEAGLGFNAT VSLGSPAVSS TEHRERNTIA 

       370 
SKSASKTESI HHR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009442 Genomic DNA. Translation: AAO28403.1.
RefSeqNP_778754.1. NC_004556.1.

3D structure databases

ProteinModelPortalQ87DZ9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING183190.PD0530.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO28403; AAO28403; PD_0530.
GeneID1144733.
KEGGxft:PD0530.
PATRIC24148753. VBIXylFas71109_0692.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0320.
KOK03644.
OMAEEYVTPE.
OrthoDBEOG6038ZS.
ProtClustDBPRK05481.

Enzyme and pathway databases

BioCycXFAS183190:GIX4-530-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_XYLFT
AccessionPrimary (citable) accession number: Q87DZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways