Q87DR8 (TRPA_XYLFT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	PD_0613

Organism

Xylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]

Taxonomic identifier

183190 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella › Xylella fastidiosa

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate By similarity. HAMAP-Rule MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Tryptophan synthase alpha chain HAMAP-Rule MF_00131		PRO_0000098879
Sites
	Active site	49	1	Proton acceptor By similarity
	Active site	60	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87DR8 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: B9603DBC89020E4C	FASTA	268	28,079
	10 20 30 40 50 60 MHRIDETFRR LRAQSRKALI PFITAGDPSL EAAVPVMHAL VRAGADVIEL GVPFSDPMAD 70 80 90 100 110 120 GPVIQHSSER ALQRGVGLAY VLQTVDVFRQ SDAVTPVVLM GYLNPLEIYG IARFTQQALA 130 140 150 160 170 180 SGVDGVLLVD LPPEEADEIR AIFSAAGLAL IVLASPTTSA SRLATLSGVA QGYLYYVSFA 190 200 210 220 230 240 GVTGADRLDA QSAGDRLRGL RAQTQVPVVV GFGIRDAASA VVMAVDADGV VVGSALVTAL 250 260 SDAPDVDTAC RRADAFLAPL RQALDAVK

References

[1]

"Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa." Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y., Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A., Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S., Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M. , Carrer H., Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L., Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L., Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S., Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F., Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G., Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A., Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L., Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P. J. Bacteriol. 185:1018-1026(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Temecula1 / ATCC 700964.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009442 Genomic DNA. Translation: AAO28485.1.
RefSeq	NP_778836.1. NC_004556.1.
3D structure databases
ProteinModelPortal	Q87DR8.
ModBase	Search...
Protein-protein interaction databases
STRING	183190.PD0613.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO28485; AAO28485; PD_0613.
GeneID	1144816.
KEGG	xft:PD0613.
PATRIC	24148974. VBIXylFas71109_0802.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0159.
KO	K01695.
OMA	TSEARAD.
ProtClustDB	PRK13111.
Enzyme and pathway databases
UniPathway	UPA00035; UER00044.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00131. Trp_synth_alpha.
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. trpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_XYLFT

Accession

Primary (citable) accession number: Q87DR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program