Reviewed, UniProtKB/Swiss-Prot Q87DN2 (PUR4_XYLFT)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	PD_0650

Organism

Xylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]

Taxonomic identifier

183190 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length	1322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1322	1322	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100427
Regions
	Domain	1073 – 1322	250	Glutamine amidotransferase type-1
	Nucleotide binding	300 – 311	12	ATP Potential
Sites
	Active site	1166	1	Nucleophile By similarity
	Active site	1287	1	By similarity
	Active site	1289	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87DN2-1 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: CE3A7CCDB06AC65B	FASTA	1,322	143,774
	10 20 30 40 50 60 MIVLKGASAL SPFRLARFES RLQTTVPELR IVGAWHCYLI QIKSGHTLDM SALHRILQAE 70 80 90 100 110 120 SVSELPQKHV VSRYVMPRLG THSPWSSKTT ELLHGADQPI ARIERGTRID LLGWSANAAT 130 140 150 160 170 180 CPAIAKQLYD PMTQSLLESE DEVKTLFNVP EPRPLERIAL IDLEHANTRL GLALTADEID 190 200 210 220 230 240 YLRTRYTELN RVPSDVELMM FAQANSEHCR HKIFNATWTI DDKEQPYSLF QMIKHTHQHT 250 260 270 280 290 300 PQYTLSAYAD NAAVIEGHPT TRYRPDPITG EYRHEAVLPG AFQIKVETHN HPTAIAPFPG 310 320 330 340 350 360 ASTGAGGEIR DEGATGRGGK PKAGLSGFSV SHLRIPTLPH PWETPRALNP RMASALDIML 370 380 390 400 410 420 EGPLGSAAFN NEFGRPNLLG YFRSFELSAS KTLTRAYDKP IMLAGGLGAI DRIHIKKLRL 430 440 450 460 470 480 QPGDVIIVLG GPAMLIGLGG GAASSVTSGT STEALDFASV QRDNPEMQRR CQEVIDHCVA 490 500 510 520 530 540 LGTNNPIRSF HDVGAGGLSN AIPELLHDSE VGGVIDLAKI PSDDPSLSPL ELWCNESQER 550 560 570 580 590 600 YVLGISAPHL QAFAAICSRE RCPFAAVGVA TATEQLIVGY GVTLPAALHV TEQQTPQRAN 610 620 630 640 650 660 RTETSPTPNT LVSPVREAFA IDLPMDVLFG KAPKMHRNTA HPPPPQWPTL DSTQLDLHHA 670 680 690 700 710 720 GLRVLAHPTV AAKNFLVTIG DRSIGGLTAR EQMIGPWQLP LADCAITLAG FSSYAGEAFA 730 740 750 760 770 780 IGERAPLALL NSAAAARMAV GEAITNLCAA PVESLSMVKL SANWMAAAEY PGEDALLYDA 790 800 810 820 830 840 VKAIGIELCP ALDISIPVGK DSLSMQSRWQ GDGATHTCIS PVSLVISAFT PVADARRQLT 850 860 870 880 890 900 PLLHHQTNSE LWLIALDGGK QRLGGSVLAQ VHADSAALPA FGGECPDLDT PETLRAFFAL 910 920 930 940 950 960 MNDARNAGLL LAYHDRSDGG AFAALCEMAF ASHLGLDITC DNRTEHLFPH LFNEELGAIV 970 980 990 1000 1010 1020 QVADEHRTAF ADLVEHHGLT AYTQRIAHPT TAPSIRVMHN DQCLAQWTWE TLFDAWWSVT 1030 1040 1050 1060 1070 1080 HAIQRLRDNP ECADEEREIA RTFTAPGLKP TLSFDPAADV AMPFISTGIR PKVAILREQG 1090 1100 1110 1120 1130 1140 INGHIEMALC FERAGFHSVD IHMNDLITGR VHLDEFVGLA ACGGFSYGDV LGAGRGWATS 1150 1160 1170 1180 1190 1200 ILERTALRDQ FAAFFTRTDR FALGVCNGCQ MLSQLKSMIP GAEHWPRFVR NRSEQFEART 1210 1220 1230 1240 1250 1260 ALLEVIQSPS IFLSGMAGSR LPVAVAHGEG YAMFDTPADQ AAAHVALRYI NGHGQAATHY 1270 1280 1290 1300 1310 1320 PLNPNGSPNG ITGLTTTDGR ITILMPHPER TPRTINLSWC PNEWGEDAPW LRLFRNARAW VG

References

[1]

"Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa." Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y., Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A., Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S., Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M. , Carrer H., Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L., Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L., Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S., Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F., Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G., Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A., Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L., Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P. J. Bacteriol. 185:1018-1026(2003) [PubMed: 12533478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009442 Genomic DNA. Translation: AAO28521.1.
RefSeq	NP_778872.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q87DN2.
Genome annotation databases
GeneID	1144853.
GenomeReviews	Gene locus PD_0650 in contig AE009442_GR.
KEGG	xft:PD0650.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q87DN2.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	XFAS183190:PD_0650-MON.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_XYLFT

Accession

Primary (citable) accession number: Q87DN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)