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Q87BL5 (SYI_XYLFT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:PD_1437
OrganismXylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]
Taxonomic identifier183190 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098510

Regions

Motif59 – 6911"HIGH" region HAMAP-Rule MF_02002
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9061Zinc By similarity
Metal binding9091Zinc By similarity
Metal binding9261Zinc By similarity
Metal binding9291Zinc By similarity
Binding site5771Aminoacyl-adenylate By similarity
Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q87BL5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8545E9D1401FC2BE

FASTA943105,633
        10         20         30         40         50         60 
MSQDYKTTLH LPATDFPMRG DLPKREPAIL ERWERDDFYA QLRAHAKGRP LFLLHDGPPY 

        70         80         90        100        110        120 
ANGQIHLGHA VNKILKDIII KSKHLDGFDA PYIPGWDCHG LPIEIAIEKK YGKVGVTLDA 

       130        140        150        160        170        180 
VQFRQKCREY AAEQIQLQRR DFKRLGIIGD WDAPYKTLDF RFEADEIRAL AKIVDKGHLI 

       190        200        210        220        230        240 
RGTKPVHWCF DCGSALAEAE IEYTDKISPM VDVAYPALDP SALAAVFNAT LPPDVQLAVP 

       250        260        270        280        290        300 
IWTTTPWTLP ASLAICVGPT LDYVLVEGPT HSGQRRWLIL AEALAAKALA RYGIAELLIH 

       310        320        330        340        350        360 
GSAKGAAMEQ HIFAHPFYPD RTIPLLLGNH VSAEDGTGAV HTAPGHGQED HQVFQQYGLL 

       370        380        390        400        410        420 
NRYSAAELNP VDARGVYLST TPPLGELTLA GLHIWKANPL IVDALRLRGV LLAAAEMHHS 

       430        440        450        460        470        480 
YPHCWRHKTP IVFRATPQWF ISMEQAALRS AALKAITHVT WYPQWGQARI LSMIENRPDW 

       490        500        510        520        530        540 
TISRQRTWGV PIPLFVHRHS GAPHPHSAAL MRQIADRVQQ QGVDIWYSLD QTELLGTEAD 

       550        560        570        580        590        600 
QYEKITDILD VWFDSGITHE AVLLERGLPK PADLYLEGAD QHRGWFQSSL LTGVAMDNAA 

       610        620        630        640        650        660 
PYKQCLTHGF TVDQHGRKMS KSLGNGIEPQ DIIKTLGADI LRLWIASTDY SNEMSLSQEI 

       670        680        690        700        710        720 
LKRTTDAYRR IRNTARFLLG NLHGFDPTLH LVPLSDMIAL DRWIVHRAFE LQQTIKAAYT 

       730        740        750        760        770        780 
RYDFAEIVQT ILNFCSVDLG SLYLDVTKDR LYTMREDAPG RRSAQTAMYH LTAAFVRWIA 

       790        800        810        820        830        840 
PILSFTADEL WSYLPGDHAD NVLFTTWYDG LAPLPPNAPL TAADFDKLLT LRDHVTKVLE 

       850        860        870        880        890        900 
PMRANGVIGA ALEAEITVAA AADTAARWQP LTEELRFLFI TGDVTVTPAN TDGFFVSAQA 

       910        920        930        940 
TTKAKCARCW HYRADIGAHP THPELCGRCI TNVDGPGEQR HWF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009442 Genomic DNA. Translation: AAO29281.1.
RefSeqNP_779632.1. NC_004556.1.

3D structure databases

ProteinModelPortalQ87BL5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING183190.PD1437.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO29281; AAO29281; PD_1437.
GeneID1144159.
KEGGxft:PD1437.
PATRIC24151070. VBIXylFas71109_1839.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycXFAS183190:GIX4-1437-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_XYLFT
AccessionPrimary (citable) accession number: Q87BL5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries