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Q87BE2 (TILS_XYLFT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA(Ile)-lysidine synthase
EC=6.3.4.19
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Gene names
Name:tilS
Ordered Locus Names:PD_1514
OrganismXylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]
Taxonomic identifier183190 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella ›

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity. HAMAP-Rule MF_01161

Catalytic activity

(tRNA(Ile2))-cytidine34 + L-lysine + ATP = (tRNA(Ile2))-lysidine34 + AMP + diphosphate + H2O. HAMAP-Rule MF_01161

Subcellular location

Cytoplasm By similarity.

Domain

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP-Rule MF_01161

Sequence similarities

Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

ligase activity, forming carbon-nitrogen bonds

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 437437tRNA(Ile)-lysidine synthase HAMAP-Rule MF_01161
PRO_0000181810

Regions

Nucleotide binding22 – 276ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q87BE2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 45D8A811F4B2C798

FASTA43748,764
        10         20         30         40         50         60 
MTVSSPFPRI PDPGVPVLVA FSGGLDSTVL LHCLASQPTQ RIHGLEAIHI HHGLNENADA 

        70         80         90        100        110        120 
WTAHCAAFCT QHDIRLHIAR VHVSHNSGQG LEAAARTARR AAFAHTLQHG HYLALAHHCD 

       130        140        150        160        170        180 
DQAETWLLRA LRGSCDGLAA MRPLTPFAAG HLWRPLLTHS RAQLLDYAQQ QHLDWIEDSS 

       190        200        210        220        230        240 
NADLRHDRNF LRIHVLPLLH QRWPQATAVL ARNAALAAAN ADLLNAEDAV LLPDLLDPDG 

       250        260        270        280        290        300 
ALDINALTAH PPARRARLLR AWCARAGAPP LPERGVNIIE RELLPARHDS AACFTWSHTE 

       310        320        330        340        350        360 
IRRWRLRLYL HRPQPPWPPD WQPLWSGTAP LILPDGGQLH LESTDHETVP GFPHPLRVRA 

       370        380        390        400        410        420 
RRGGERLILP GRTHSHPLKH LLQDVGIPPW RRASMPLLCD GEQILAVGDA LLAAPLVTWL 

       430 
QAHRLKLRWQ YHNNTCI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE009442 Genomic DNA. Translation: AAO29357.1.
RefSeqNP_779708.1. NC_004556.1.

3D structure databases

ProteinModelPortalQ87BE2.
ModBaseSearch...

Protein-protein interaction databases

STRING183190.PD1514.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO29357; AAO29357; PD_1514.
GeneID1142856.
KEGGxft:PD1514.
PATRIC24151254. VBIXylFas71109_1932.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0037.
KOK04075.
OMASNQDDAY.
ProtClustDBCLSK446060.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_01161. tRNA(Ile)-lys_synt.
InterProIPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR015262. Lysidine-tRNA-synth_subst-bd.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PANTHERPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMSSF56037. B3_4. 1 hit.
TIGRFAMsTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTILS_XYLFT
AccessionPrimary (citable) accession number: Q87BE2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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