Q87AR1 (RSMB_XYLFT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase B
EC=2.1.1.176

Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB

Gene names

Name:	rsmB
Synonyms:	rrmB
Ordered Locus Names:	PD_1761

Organism

Xylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]

Taxonomic identifier

183190 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella › Xylella fastidiosa

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA methylation Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Ribosomal RNA small subunit methyltransferase B		PRO_0000211813
Regions
	Region	249 – 255	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	370	1	Nucleophile Potential
	Binding site	272	1	S-adenosyl-L-methionine By similarity
	Binding site	298	1	S-adenosyl-L-methionine By similarity
	Binding site	317	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q87AR1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 35C7C763BAB0908B	FASTA	431	46,932
	10 20 30 40 50 60 MSDGIAPRVV AARVLALVVD QGRSLKTELA AALPTLEDVR DRALVEAICF AVLRRRPVYE 70 80 90 100 110 120 AALTRWLARP LGRGDAQLRG LLMVGFAQLD VLKVPPYAAL SATVDACRVL GWPHRVSFVN 130 140 150 160 170 180 AVLRRAQRER LPVVSSDAAW PRWLAERIRA DWGEQAEAIF DASLKPAPMW LRVNLRYGDR 190 200 210 220 230 240 HTYVQRLQAA GLEAVPSGLV PEALALDCSV PVVQLPGFQG GEVSVQDLSA QQVAALLSPA 250 260 270 280 290 300 PHARLLDACA APGGKAAHLL ERAPTLCLTA LDVDMQRLRR VAETCDRIHV KARLCVADAT 310 320 330 340 350 360 DLAAWWDGEP FDAVLLDAPC SATGVVRRQP DVLLHRRAED LLPLLNIQSR LLDACWRTLR 370 380 390 400 410 420 PGGVLVYVTC SVLRAENQTQ LEAFLARTAD ACAEDPGDAY GQPAGLGRQR LPGEQGGDGF 430 FYARLIKDIS M

References

[1]

"Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa." Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y., Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A., Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S., Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M. , Carrer H., Carraro D.M., de Oliveira R.C., Nunes L.R., Siqueira W.J., Coutinho L.L., Kimura E.T., Ferro E.S., Harakava R., Kuramae E.E., Marino C.L., Giglioti E., Abreu I.L., Alves L.M.C., do Amaral A.M., Baia G.S., Blanco S.R., Brito M.S., Cannavan F.S., Celestino A.V., da Cunha A.F., Fenille R.C., Ferro J.A., Formighieri E.F., Kishi L.T., Leoni S.G., Oliveira A.R., Rosa V.E. Jr., Sassaki F.T., Sena J.A.D., de Souza A.A., Truffi D., Tsukumo F., Yanai G.M., Zaros L.G., Civerolo E.L., Simpson A.J.G., Almeida N.F. Jr., Setubal J.C., Kitajima J.P. J. Bacteriol. 185:1018-1026(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Temecula1 / ATCC 700964.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009442 Genomic DNA. Translation: AAO29595.1.
RefSeq	NP_779946.1. NC_004556.1.
3D structure databases
ProteinModelPortal	Q87AR1.
ModBase	Search...
Protein-protein interaction databases
STRING	183190.PD1761.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO29595; AAO29595; PD_1761.
GeneID	1143103.
KEGG	xft:PD1761.
PATRIC	24151965. VBIXylFas71109_2282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0144.
KO	K03500.
OMA	KPPMWLR.
ProtClustDB	PRK10901.
Family and domain databases
Gene3D	1.10.940.10. 1 hit.
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. IPR006027. NusB_RsmB_TIM44. IPR023267. RCMT. IPR004573. rRNA_ssu_MeTfrase_B. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. PF01029. NusB. 1 hit. [Graphical view]
PRINTS	PR02008. RCMTFAMILY.
SUPFAM	SSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMs	TIGR00563. rsmB. 1 hit.
PROSITE	PS01153. NOL1_NOP2_SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMB_XYLFT

Accession

Primary (citable) accession number: Q87AR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program