Phenylalanine--tRNA ligase beta subunit - Xylella fastidiosa (strain Temecula1 / ATCC 700964)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	PD_1911

Organism

Xylella fastidiosa (strain Temecula1 / ATCC 700964) [Complete proteome] [HAMAP]

Taxonomic identifier

183190 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella ›

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 792

792

Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283

PRO_0000126992

Regions

Domain

39 – 147

109

tRNA-binding

Domain

400 – 475

76

B5

Domain

698 – 791

94

FDX-ACB

Sites

Metal binding

453

1

Magnesium By similarity

Metal binding

459

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

462

1

Magnesium By similarity

Metal binding

463

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q87AB6 [UniParc].

Last modified June 16, 2003. Version 1.
Checksum: CD94C012C9625516
Show »

FASTA

792

86,424

        10         20         30         40         50         60 
MKFSENWLRN HAPIQANRDV LVATLTAIGL EVENVAVLGE ALDLILVARI VNVVPHPESD 

        70         80         90        100        110        120 
LLQICQVDVA QDTLLQIVCG ASNVRPGLVV PLALLGAKIG ALTIKSTTLR GIESNGMLCS 

       130        140        150        160        170        180 
AKELGLDTEA SGLMELPEDA PIGTPLADYL ALPDASIEIK LTPNRADCFS VRGIAFDVAA 

       190        200        210        220        230        240 
ACASEVTPFH IDEIPAVSAR TLPVELHAGA NAPRYCGCVI EGIDPAAPTP VWMAERLRRS 

       250        260        270        280        290        300 
GIRPVSLLVD ITQYVMLELG QPMHAFDVDT LRGPIGVRLS RNDEALKLLD GRTVVLDNDF 

       310        320        330        340        350        360 
LVVTDADQPI ALAGLIGGWE TRITDTTINV FLEAAHFAPA AIMGRGRKLG LHTDASHRFE 

       370        380        390        400        410        420 
RGVDPALPPQ AIAFATRLIL ELAGGKPGSL IHVQLPEYLP APASILLRRT RIARLLGIVI 

       430        440        450        460        470        480 
DDAEVERILQ ALGMQVTTQA EGWRVVAPSR RFDIAIEEDL IEELVRIRGY EHLPTALPVG 

       490        500        510        520        530        540 
ASHIAMPSET RLDMTSVRRQ LIARELQETI NYAFIDAELL RRWQLNTGQV MLMNPLSAEL 

       550        560        570        580        590        600 
AVMRPRLLPG LVAALGRNIA RQLERVRLFE LGNVFTASDE AGAAPLETRH VAAAVCGDAF 

       610        620        630        640        650        660 
ALQWGEQVRK VDFYDLKGDL ESLAAASGAV LTFHSSAQPW GHPGRSADVW CDDMCIGWIG 

       670        680        690        700        710        720 
QLHPALTQTL EINVDVIAFE LALEPLVRRA LPRAHALSRF PFVRRDLACV VPEHVTWSEL 

       730        740        750        760        770        780 
AITVRDVIGP LLRDVKLFDR YVGKGIEPGF KSLAIGLILQ DDTRTLIDRD VDDIMTKVVM 

       790 
AIQQRHDVRI RS

« Hide

References

[1]

"Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa."
Van Sluys M.A., de Oliveira M.C., Monteiro-Vitorello C.B., Miyaki C.Y., Furlan L.R., Camargo L.E.A., da Silva A.C.R., Moon D.H., Takita M.A., Lemos E.G.M., Machado M.A., Ferro M.I.T., da Silva F.R., Goldman M.H.S., Goldman G.H., Lemos M.V.F., El-Dorry H., Tsai S.M.

Kitajima J.P.
J. Bacteriol. 185:1018-1026(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Temecula1 / ATCC 700964.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009442 Genomic DNA. Translation: AAO29741.1.
RefSeq	NP_780092.1. NC_004556.1.
3D structure databases
ProteinModelPortal	Q87AB6.
ModBase	Search...
Protein-protein interaction databases
STRING	183190.PD1911.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO29741; AAO29741; PD_1911.
GeneID	1143253.
KEGG	xft:PD1911.
PATRIC	24152346. VBIXylFas71109_2469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0073.
KO	K01890.
OMA	MKFSEQW.
ProtClustDB	PRK00629.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.56.20. 1 hit. 3.30.70.380. 1 hit. 3.50.40.10. 1 hit.
HAMAP	MF_00283. Phe_tRNA_synth_beta1.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. pheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_XYLFT

Accession

Primary (citable) accession number: Q87AB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents