Reviewed, UniProtKB/Swiss-Prot Q876L2 (ARE2_SACBA)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 2
    EC=2.3.1.26
Alternative name(s):
    Sterol-ester synthase 2

Gene names

Name:

ARE2

Organism

Saccharomyces bayanus (Yeast) (Saccharomyces uvarum)

Taxonomic identifier

4931 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Sterol O-acyltransferase 2		PRO_0000207650
Regions
	Transmembrane	223 – 243	21	Potential
	Transmembrane	300 – 320	21	Potential
	Transmembrane	412 – 432	21	Potential
	Transmembrane	450 – 470	21	Potential
	Transmembrane	493 – 513	21	Potential
	Transmembrane	575 – 595	21	Potential
	Transmembrane	630 – 650	21	Potential
Sites
	Active site	587	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q876L2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F1B6654C6D9BC86	FASTA	650	74,610
	10 20 30 40 50 60 MDKNKDLLEN EQFLRIQKLN ASDAGKRQSI LVDNEDELYG LTSSNNSCAS EHEGEGEGED 70 80 90 100 110 120 ERPATTSSAP TQNHSAGDVA FIPGKTAEED TETVTKVVES DDQVFRTHVQ TLSSKGKSRY 130 140 150 160 170 180 RKGSSNFISF FDDMAFENRP SILDGSVNDP FKTKFVGPTL EKEIRKREKE LMAMRKNLHH 190 200 210 220 230 240 GKPAPDADAA DAPALTTTTT TTTSATSPET VVTIETTILS SNFSGLYVAF WMAIAFGAVK 250 260 270 280 290 300 ALIDYYYQHN GSFKDSEILK FMTTNLSTVA LIDLIMYLST FFVVGIQYLC KWGVLNWSST 310 320 330 340 350 360 GWAFTSIYEL LFVGFYMYLT ENILKLHWLS KIFLFLHSLV LLMKMHSFAF YNGYLWGIKQ 370 380 390 400 410 420 ELQFSKSALA KYKDSVNDPD VVDALEKSCE FCSFELNSQS LNDQTQKFPN NINVSNFFMF 430 440 450 460 470 480 TMFPTLIYQI EYPRTKEIRW VYVLEKICAI FGTIFLMMID AQILMHPVAM RALDVRNSEW 490 500 510 520 530 540 TGILDRLLKW AGLLVDIVPG FIVMYILDFY LIWDAILNCV AELTRFGDRY FYGDWWNCVS 550 560 570 580 590 600 WADFSRIWNI PVHKFLLRHV YHSSMSSFKL NKSQATLMTF FLSSVVHELA MYVIFKRLRF 610 620 630 640 650 YLFFFQMLQV PLVALTNTKY MKDRTVIGNV IFWLGICMGP SVMCTLYLTF

References

[1]	"Yeast genome duplication was followed by asynchronous differentiation of duplicated genes." Langkjaer R.B., Cliften P.F., Johnston M., Piskur J. Nature 421:848-852(2003) [PubMed: 12594514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 623-6C.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY144799 Genomic DNA. Translation: AAO32363.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.26. 164752.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARE2_SACBA

Accession

Primary (citable) accession number: Q876L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 9, 2003
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)