Q875Z9 (KPYK_NAUCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	PYK1
Synonyms:	CDC19
Ordered Locus Names:	NCAS_0A07590

Organism

Naumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii) [Complete proteome]

Taxonomic identifier

1064592 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Naumovozyma › Naumovozyma castellii

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Pyruvate kinase		PRO_0000112116
Sites
	Metal binding	52	1	Potassium By similarity
	Metal binding	54	1	Potassium By similarity
	Metal binding	85	1	Potassium By similarity
	Metal binding	86	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	243	1	Magnesium Potential
	Metal binding	267	1	Magnesium By similarity
	Binding site	50	1	Substrate By similarity
	Binding site	266	1	Substrate; via amide nitrogen By similarity
	Binding site	267	1	Substrate; via amide nitrogen By similarity
	Binding site	299	1	Substrate By similarity
	Binding site	338	1	ADP Potential
	Site	241	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q875Z9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6624A308CE8A2F75	FASTA	501	54,463
	10 20 30 40 50 60 MESRLARLTT LQVTAGDNLR RTSIIGTIGP KTNNPEVLVA LRKAGLNIVR MNFSHGSYEY 70 80 90 100 110 120 HQSVIENARK SEELYPGRPL AIALDTKGPE IRTGTTTGEV DYPIPPNHEM IFSTDEKYAK 130 140 150 160 170 180 ACDDKVMFVD YANITKVISK GKIIYVDDGV LSFEVLEVVD GKTLKVKSLN AGKICSHKGV 190 200 210 220 230 240 NLPGTDVDLP ALSEKDKADL RFGVKNGVHM VFASFIRTAQ DVLTIREVLG EDGKDIKIVV 250 260 270 280 290 300 KIENQQGVNN FDEILKVTDA VMVARGDLGI EIPAPEVLAV QKKLIAKSNL AGKPVICATQ 310 320 330 340 350 360 MLESMTYNPR PTRAEVSDVG NAILDGADCV MLSGETAKGN YPINAVTTMA DTALIAEQAI 370 380 390 400 410 420 AYQPLYDDLR NLTPKPTSTT ETVAASAVAA VYEQKAKAII VLSTSGTTPR LVSKYRPDCP 430 440 450 460 470 480 IILVTRNPRA ARFSHLSRGV FPFVYEADSV ADWTEDVELR LKFGIEKAIE MGVMKKGDTY 490 500 VSIQGFKAGE GHSNTLQVSN A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast genome duplication was followed by asynchronous differentiation of duplicated genes." Langkjaer R.B., Cliften P.F., Johnston M., Piskur J. Nature 421:848-852(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630.
[2]	"Genome sequence of Naumovozyma castellii." Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P., Wolfe K.H. Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY144916 Genomic DNA. Translation: AAO32480.1. HE576752 Genomic DNA. Translation: CCC67317.1.
RefSeq	XP_003673698.1. XM_003673650.1.
3D structure databases
ProteinModelPortal	Q875Z9.
SMR	Q875Z9. Positions 3-499.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	11526928.
KEGG	ncs:NCAS_0A07590.
Phylogenomic databases
KO	K00873.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_NAUCC

Accession

Primary (citable) accession number: Q875Z9
Secondary accession number(s): G0V769

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program