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Protein

Pyruvate kinase

Gene

PYK1

Organism
Naumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Metal bindingi52 – 521PotassiumBy similarity
Metal bindingi54 – 541PotassiumBy similarity
Metal bindingi85 – 851PotassiumBy similarity
Metal bindingi86 – 861Potassium; via carbonyl oxygenBy similarity
Sitei241 – 2411Transition state stabilizerBy similarity
Metal bindingi243 – 2431MagnesiumSequence Analysis
Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
Metal bindingi267 – 2671MagnesiumBy similarity
Binding sitei267 – 2671Substrate; via amide nitrogenBy similarity
Binding sitei299 – 2991SubstrateBy similarity
Binding sitei338 – 3381ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Synonyms:CDC19
Ordered Locus Names:NCAS_0A07590
OrganismiNaumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)
Taxonomic identifieri1064592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNaumovozyma
ProteomesiUP000001640 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Pyruvate kinasePRO_0000112116Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ875Z9.
SMRiQ875Z9. Positions 3-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

InParanoidiQ875Z9.
KOiK00873.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q875Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRLARLTT LQVTAGDNLR RTSIIGTIGP KTNNPEVLVA LRKAGLNIVR
60 70 80 90 100
MNFSHGSYEY HQSVIENARK SEELYPGRPL AIALDTKGPE IRTGTTTGEV
110 120 130 140 150
DYPIPPNHEM IFSTDEKYAK ACDDKVMFVD YANITKVISK GKIIYVDDGV
160 170 180 190 200
LSFEVLEVVD GKTLKVKSLN AGKICSHKGV NLPGTDVDLP ALSEKDKADL
210 220 230 240 250
RFGVKNGVHM VFASFIRTAQ DVLTIREVLG EDGKDIKIVV KIENQQGVNN
260 270 280 290 300
FDEILKVTDA VMVARGDLGI EIPAPEVLAV QKKLIAKSNL AGKPVICATQ
310 320 330 340 350
MLESMTYNPR PTRAEVSDVG NAILDGADCV MLSGETAKGN YPINAVTTMA
360 370 380 390 400
DTALIAEQAI AYQPLYDDLR NLTPKPTSTT ETVAASAVAA VYEQKAKAII
410 420 430 440 450
VLSTSGTTPR LVSKYRPDCP IILVTRNPRA ARFSHLSRGV FPFVYEADSV
460 470 480 490 500
ADWTEDVELR LKFGIEKAIE MGVMKKGDTY VSIQGFKAGE GHSNTLQVSN

A
Length:501
Mass (Da):54,463
Last modified:May 31, 2003 - v1
Checksum:i6624A308CE8A2F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY144916 Genomic DNA. Translation: AAO32480.1.
HE576752 Genomic DNA. Translation: CCC67317.1.
RefSeqiXP_003673698.1. XM_003673650.1.

Genome annotation databases

GeneIDi11526928.
KEGGincs:NCAS_0A07590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY144916 Genomic DNA. Translation: AAO32480.1.
HE576752 Genomic DNA. Translation: CCC67317.1.
RefSeqiXP_003673698.1. XM_003673650.1.

3D structure databases

ProteinModelPortaliQ875Z9.
SMRiQ875Z9. Positions 3-499.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11526928.
KEGGincs:NCAS_0A07590.

Phylogenomic databases

InParanoidiQ875Z9.
KOiK00873.
OrthoDBiEOG7M6DJC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast genome duplication was followed by asynchronous differentiation of duplicated genes."
    Langkjaer R.B., Cliften P.F., Johnston M., Piskur J.
    Nature 421:848-852(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630.
  2. "Genome sequence of Naumovozyma castellii."
    Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P., Wolfe K.H.
    Submitted (JUN-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630.

Entry informationi

Entry nameiKPYK_NAUCC
AccessioniPrimary (citable) accession number: Q875Z9
Secondary accession number(s): G0V769
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2004
Last sequence update: May 31, 2003
Last modified: January 6, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.