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Protein

Pyruvate kinase

Gene

PYK1

Organism
Naumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (NCAS0D01840), Glyceraldehyde-3-phosphate dehydrogenase (NCAS0A07140), Glyceraldehyde-3-phosphate dehydrogenase (NCAS0A10810)
  2. no protein annotated in this organism
  3. Phosphoglycerate mutase (NCAS0F02370), Phosphoglycerate mutase (NCAS0C03650)
  4. no protein annotated in this organism
  5. Pyruvate kinase (PYK1), Pyruvate kinase (NCAS0E02380)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Metal bindingi52PotassiumBy similarity1
Metal bindingi54PotassiumBy similarity1
Metal bindingi85PotassiumBy similarity1
Metal bindingi86Potassium; via carbonyl oxygenBy similarity1
Sitei241Transition state stabilizerBy similarity1
Metal bindingi243MagnesiumSequence analysis1
Binding sitei266Substrate; via amide nitrogenBy similarity1
Metal bindingi267MagnesiumBy similarity1
Binding sitei267Substrate; via amide nitrogenBy similarity1
Binding sitei299SubstrateBy similarity1
Binding sitei338ADPSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Synonyms:CDC19
Ordered Locus Names:NCAS_0A07590
OrganismiNaumovozyma castellii (strain ATCC 76901 / CBS 4309 / NBRC 1992 / NRRL Y-12630) (Yeast) (Saccharomyces castellii)
Taxonomic identifieri1064592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNaumovozyma
Proteomesi
  • UP000001640 Componenti: Chromosome 1

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121161 – 501Pyruvate kinaseAdd BLAST501

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi1064592.XP_003673698.1.

Structurei

3D structure databases

ProteinModelPortaliQ875Z9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
InParanoidiQ875Z9.
KOiK00873.
OrthoDBiEOG092C1RXZ.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q875Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRLARLTT LQVTAGDNLR RTSIIGTIGP KTNNPEVLVA LRKAGLNIVR
60 70 80 90 100
MNFSHGSYEY HQSVIENARK SEELYPGRPL AIALDTKGPE IRTGTTTGEV
110 120 130 140 150
DYPIPPNHEM IFSTDEKYAK ACDDKVMFVD YANITKVISK GKIIYVDDGV
160 170 180 190 200
LSFEVLEVVD GKTLKVKSLN AGKICSHKGV NLPGTDVDLP ALSEKDKADL
210 220 230 240 250
RFGVKNGVHM VFASFIRTAQ DVLTIREVLG EDGKDIKIVV KIENQQGVNN
260 270 280 290 300
FDEILKVTDA VMVARGDLGI EIPAPEVLAV QKKLIAKSNL AGKPVICATQ
310 320 330 340 350
MLESMTYNPR PTRAEVSDVG NAILDGADCV MLSGETAKGN YPINAVTTMA
360 370 380 390 400
DTALIAEQAI AYQPLYDDLR NLTPKPTSTT ETVAASAVAA VYEQKAKAII
410 420 430 440 450
VLSTSGTTPR LVSKYRPDCP IILVTRNPRA ARFSHLSRGV FPFVYEADSV
460 470 480 490 500
ADWTEDVELR LKFGIEKAIE MGVMKKGDTY VSIQGFKAGE GHSNTLQVSN

A
Length:501
Mass (Da):54,463
Last modified:June 1, 2003 - v1
Checksum:i6624A308CE8A2F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY144916 Genomic DNA. Translation: AAO32480.1.
HE576752 Genomic DNA. Translation: CCC67317.1.
RefSeqiXP_003673698.1. XM_003673650.1.

Genome annotation databases

EnsemblFungiiCCC67317; CCC67317; NCAS_0A07590.
GeneIDi11526928.
KEGGincs:NCAS_0A07590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY144916 Genomic DNA. Translation: AAO32480.1.
HE576752 Genomic DNA. Translation: CCC67317.1.
RefSeqiXP_003673698.1. XM_003673650.1.

3D structure databases

ProteinModelPortaliQ875Z9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1064592.XP_003673698.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCCC67317; CCC67317; NCAS_0A07590.
GeneIDi11526928.
KEGGincs:NCAS_0A07590.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
InParanoidiQ875Z9.
KOiK00873.
OrthoDBiEOG092C1RXZ.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_NAUCC
AccessioniPrimary (citable) accession number: Q875Z9
Secondary accession number(s): G0V769
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: October 5, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.