Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

PYK1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (KLLA0_F09141g), Glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1), Glyceraldehyde-3-phosphate dehydrogenase 2 (GAP2)
  2. Phosphoglycerate kinase (PGK)
  3. Phosphoglycerate mutase (KLLA0_E11859g), Phosphoglycerate mutase (KLLA0_C04081g)
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Metal bindingi52PotassiumBy similarity1
Metal bindingi54PotassiumBy similarity1
Metal bindingi85PotassiumBy similarity1
Metal bindingi86Potassium; via carbonyl oxygenBy similarity1
Sitei241Transition state stabilizerBy similarity1
Metal bindingi243MagnesiumSequence analysis1
Binding sitei266Substrate; via amide nitrogenBy similarity1
Metal bindingi267MagnesiumBy similarity1
Binding sitei267Substrate; via amide nitrogenBy similarity1
Binding sitei299SubstrateBy similarity1
Binding sitei338ADPSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Synonyms:CDC19
Ordered Locus Names:KLLA0F23397g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome F

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121141 – 501Pyruvate kinaseAdd BLAST501

Proteomic databases

PRIDEiQ875M9.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_456122.1.

Structurei

3D structure databases

ProteinModelPortaliQ875M9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ875M9.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiEOG092C1RXZ.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q875M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRLGWLTD LSTETGTNLR RTSIICTIGP KTNNPETLVE LRKAGMNIVR
60 70 80 90 100
MNFSHGSYEY HQSVIDNARK SEELYQGRPL AIALDTKGPE IRTGTTTNDV
110 120 130 140 150
DYPIPPNHEM IFTTDDKYAK ACDDKTMYVD YKNITKVIEA GRIIYVDDGV
160 170 180 190 200
LSFEVLEVID DNTLKVKSLN AGKICSHKGV NLPGTDVDLP ALSEKDKSDL
210 220 230 240 250
KFGVKNGVHM VFASFIRTAQ DVLTIREVLG EQGKDIKIIV KIENQQGVNN
260 270 280 290 300
FDDILKVTDG VMVARGDLGI EIPAPQVFAV QKKLIAKCNL AGKPVICATQ
310 320 330 340 350
MLESMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGN YPINAVKTMA
360 370 380 390 400
ETALIAEQAI PYIPTYDDLR NLTPKPTSTT ETIAAASVSA VFEQKARALI
410 420 430 440 450
VLSTTGDTPR LVAKYKPNVP IVMVTRNPRA ARFSHLYRGV FPFVYDESSD
460 470 480 490 500
SEWTVDVEKR INFGVKKAKE FGILVDGDTI VTIQGFAAGV GNSNTLRVLT

V
Length:501
Mass (Da):54,917
Last modified:June 1, 2003 - v1
Checksum:i12F2D4C3E4940B95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145040 Genomic DNA. Translation: AAO32602.1.
CR382126 Genomic DNA. Translation: CAG98830.1.
RefSeqiXP_456122.1. XM_456122.1.

Genome annotation databases

EnsemblFungiiCAG98830; CAG98830; KLLA0_F23397g.
GeneIDi2895322.
KEGGikla:KLLA0F23397g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145040 Genomic DNA. Translation: AAO32602.1.
CR382126 Genomic DNA. Translation: CAG98830.1.
RefSeqiXP_456122.1. XM_456122.1.

3D structure databases

ProteinModelPortaliQ875M9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_456122.1.

Proteomic databases

PRIDEiQ875M9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG98830; CAG98830; KLLA0_F23397g.
GeneIDi2895322.
KEGGikla:KLLA0F23397g.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ875M9.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiEOG092C1RXZ.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_KLULA
AccessioniPrimary (citable) accession number: Q875M9
Secondary accession number(s): Q6CIW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: October 5, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.