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Protein

Pyruvate kinase

Gene

PYK1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (KLLA0_F09141g), Glyceraldehyde-3-phosphate dehydrogenase 2 (GAP2), Glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1)
  2. Phosphoglycerate kinase (PGK)
  3. Phosphoglycerate mutase (KLLA0_E11859g), Phosphoglycerate mutase (KLLA0_C04081g)
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Metal bindingi52PotassiumBy similarity1
Metal bindingi54PotassiumBy similarity1
Metal bindingi85PotassiumBy similarity1
Metal bindingi86Potassium; via carbonyl oxygenBy similarity1
Sitei241Transition state stabilizerBy similarity1
Metal bindingi243MagnesiumSequence analysis1
Binding sitei266Substrate; via amide nitrogenBy similarity1
Metal bindingi267MagnesiumBy similarity1
Binding sitei267Substrate; via amide nitrogenBy similarity1
Binding sitei299SubstrateBy similarity1
Binding sitei338ADPSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Synonyms:CDC19
Ordered Locus Names:KLLA0F23397g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome F

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121141 – 501Pyruvate kinaseAdd BLAST501

Proteomic databases

PRIDEiQ875M9

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_456122.1

Structurei

3D structure databases

ProteinModelPortaliQ875M9
SMRiQ875M9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
InParanoidiQ875M9
KOiK00873
OMAiKHEAIEQ
OrthoDBiEOG092C1RXZ

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q875M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRLGWLTD LSTETGTNLR RTSIICTIGP KTNNPETLVE LRKAGMNIVR
60 70 80 90 100
MNFSHGSYEY HQSVIDNARK SEELYQGRPL AIALDTKGPE IRTGTTTNDV
110 120 130 140 150
DYPIPPNHEM IFTTDDKYAK ACDDKTMYVD YKNITKVIEA GRIIYVDDGV
160 170 180 190 200
LSFEVLEVID DNTLKVKSLN AGKICSHKGV NLPGTDVDLP ALSEKDKSDL
210 220 230 240 250
KFGVKNGVHM VFASFIRTAQ DVLTIREVLG EQGKDIKIIV KIENQQGVNN
260 270 280 290 300
FDDILKVTDG VMVARGDLGI EIPAPQVFAV QKKLIAKCNL AGKPVICATQ
310 320 330 340 350
MLESMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGN YPINAVKTMA
360 370 380 390 400
ETALIAEQAI PYIPTYDDLR NLTPKPTSTT ETIAAASVSA VFEQKARALI
410 420 430 440 450
VLSTTGDTPR LVAKYKPNVP IVMVTRNPRA ARFSHLYRGV FPFVYDESSD
460 470 480 490 500
SEWTVDVEKR INFGVKKAKE FGILVDGDTI VTIQGFAAGV GNSNTLRVLT

V
Length:501
Mass (Da):54,917
Last modified:June 1, 2003 - v1
Checksum:i12F2D4C3E4940B95
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY145040 Genomic DNA Translation: AAO32602.1
CR382126 Genomic DNA Translation: CAG98830.1
RefSeqiXP_456122.1, XM_456122.1

Genome annotation databases

EnsemblFungiiCAG98830; CAG98830; KLLA0_F23397g
GeneIDi2895322
KEGGikla:KLLA0F23397g

Similar proteinsi

Entry informationi

Entry nameiKPYK_KLULA
AccessioniPrimary (citable) accession number: Q875M9
Secondary accession number(s): Q6CIW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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