Q874R1 (RHO4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP-binding protein rho4

Gene names

Name:	rho4
ORF Names:	SPAC16A10.04

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for cell separation. Involved in the regulation of the septum degradation during cytokinesis and in the organization of F-actin patches and cytoplasmic microtubules. Ref.1 Ref.2
Subcellular location	Membrane. Note: Membrane-bound. Associates with the septum during mitosis. Ref.1 Ref.2
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cellular protein localization Inferred from mutant phenotype PubMed 16215171. Source: PomBase fungal-type cell wall organization or biogenesis Traceable author statement Ref.1. Source: PomBase microtubule cytoskeleton organization Inferred from mutant phenotype Ref.2. Source: PomBase primary cell septum disassembly Traceable author statement Ref.1. Source: PomBase regulation of actin cytoskeleton organization Inferred from mutant phenotype Ref.2. Source: PomBase regulation of barrier septum assembly Inferred from mutant phenotype Ref.2. Source: PomBase regulation of cell shape Inferred from mutant phenotype Ref.2. Source: PomBase small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	barrier septum Inferred from direct assay Ref.2. Source: PomBase cell cortex Inferred from direct assay Ref.2. Source: PomBase membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred by curator. Source: PomBase GTPase activity Inferred from direct assay PubMed 18793338. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	GTP-binding protein rho4		PRO_0000198943
	Propeptide	201 – 203	3	Removed in mature form By similarity		PRO_0000281273
Regions
	Nucleotide binding	21 – 28	8	GTP By similarity
	Nucleotide binding	70 – 74	5	GTP By similarity
	Motif	43 – 51	9	Effector region By similarity
Amino acid modifications
	Modified residue	200	1	Cysteine methyl ester By similarity
	Lipidation	200	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	23	1	G → V: Cell lysis during separation. Ref.1 Ref.2
	Mutagenesis	28	1	T → N: No cell lysis. Ref.1
	Mutagenesis	74	1	Q → L: Shrunken cells. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q874R1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 69025B96208649F9	FASTA	203	22,408
	10 20 30 40 50 60 MSAFKKSGSK SETSKKLVVV GDGGCGKTCL LIVFSSGTFP ERYVPTVFEN YITDITYGPN 70 80 90 100 110 120 SKVIELALWD TAGQEEYDRL RPLSYPNSNV ILLCFSIDCP ASLNNVTEKW YPEVQHFCPR 130 140 150 160 170 180 TPIVLVGLKA DLRKDRNATE VLRTQGLTPV TYQQAQSVAL SMNAPYVECS AKENTGVNEV 190 200 FQLAVGLTIK KSFSFSKKSC VIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel Rho GTPase involved in cytokinesis and cell wall integrity in the fission yeast Schizosaccharomyces pombe." Santos B., Gutierrez J., Calonge T.M., Perez P. Eukaryot. Cell 2:521-533(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-23 AND THR-28.
[2]	"The small GTPase Rho4 is involved in controlling cell morphology and septation in fission yeast." Nakano K., Mutoh T., Arai R., Mabuchi I. Genes Cells 8:357-370(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-23 AND GLN-74.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAD86931.2.
RefSeq	NP_001018257.1. NM_001019469.2.
3D structure databases
ProteinModelPortal	Q874R1.
SMR	Q874R1. Positions 15-184.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC16A10.04-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC16A10.04.1; SPAC16A10.04.1:pep; SPAC16A10.04.
GeneID	3361476.
KEGG	spo:SPAC16A10.04.
Organism-specific databases
PomBase	SPAC16A10.04.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233974.
KO	K07975.
OMA	TESHIIL.
OrthoDB	EOG498Z8Z.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20811519.

Entry information

Entry name

RHO4_SCHPO

Accession

Primary (citable) accession number: Q874R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	May 29, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program