Reviewed, UniProtKB/Swiss-Prot Q874K0 (ERG27_CANAL)

Names and origin

Protein names

Recommended name:
    3-keto-steroid reductase
    EC=1.1.1.270

Gene names

Name:

ERG27

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto-steroid reductase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	3-keto-steroid reductase		PRO_0000054590
Sites
	Active site	205	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q874K0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F9285E5FB3717827	FASTA	346	39,078
	10 20 30 40 50 60 MSLLKDSTVA VITGTSSNLG FNIAVRLLEG LPDNKEITLV VTSRTLPKVK EVISDIKKYI 70 80 90 100 110 120 VEKIPTKVNK VEFDYLLVDF TDMVSILSAY YELNKRYKHI DYLFINAAQG VYGGIDWTGA 130 140 150 160 170 180 VLEVLQSPIE AVTNPTYKLQ KVGVESGDKL GLVFQANVFG PYYFIHRIKH LLKNGGKIVW 190 200 210 220 230 240 ISSLMSSPKY LSFNDLQLLR SPASYEGSKR LVDLMHFGTY NKLEREYGIK QYLVHPGIFT 250 260 270 280 290 300 SFSFFQYLNV FTYYGMLFLF YLARFLGSPY HNISGYIAAN APVAAALGQT KQNCKTASAC 310 320 330 340 TRSGKEYLLE EEIDSTGSDD VVSYLDTLTK EWDEKLKDQI VNTRQP

References

[1]

"Isolation, characterization, and regulation of the Candida albicans ERG27 gene encoding the sterol 3-keto reductase." Pierson C.A., Jia N., Mo C., Lees N.D., Sturm A.M., Eckstein J., Barbuct R., Bard M. Med. Mycol. 42:461-473(2004) [PubMed: 15552648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY140908 Genomic DNA. Translation: AAN28009.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.270. 1124.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ERG27_CANAL

Accession

Primary (citable) accession number: Q874K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)