Reviewed, UniProtKB/Swiss-Prot Q873E8 (GSHR_NEUCR)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:	glr-1
ORF Names:	B10C3.130, NCU03339

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol By similarity.
Catalytic activity	2 glutathione + NADP(+) = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Miscellaneous	The active site is a redox-active disulfide bond By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Glutathione reductase		PRO_0000067969
Regions
	Nucleotide binding	38 – 46	9	FAD By similarity
Sites
	Active site	457	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	46 ↔ 51		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q873E8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A3802C1A7DC0B479	FASTA	468	50,313
	10 20 30 40 50 60 MAPISRETDF LVIGGGSGGI ATARAAAGKY GIKSMVVEGK RLGGTCVNVG CVPKKVTFYA 70 80 90 100 110 120 ALVAETIHQA KDYGFSVEQT APFDWPTFKQ KRDAYVARLN GIYERNLAND KVEYVHGWAK 130 140 150 160 170 180 LLSPNSVEVT LDDGTKSVVN AKKILIAVGG NPTIPPHIPG SEYGTNSDGF FDIDTLPKKV 190 200 210 220 230 240 ALVGAGYIAV EFAGMLNALG VETHLFIRHD TFLRSFDPMI QQVSVKEYER IGVKVHKKSQ 250 260 270 280 290 300 LTSVQKDAAG KLAINFKEGE GEQSISDVDH LIWAVGRTPA VEGLGLDKAG VKTNEKGYIE 310 320 330 340 350 360 VDEYQNTSTE NIYAVGDVCG QVELTPVAIA AGRKLAARLF GPEEFRTLKL NYDNVPSVVF 370 380 390 400 410 420 AHPEIGSIGL TEPEAVAKYG AENLKIYKSS FTAMYYAMMK PEDKAPTAYK LICAGPEEKV 430 440 450 460 VGLHIIGLGS GEILQGFGVA VNMGATKADF DNCVAIHPTS AEELVTLK

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX284749 Genomic DNA. Translation: CAD70360.1. AABX02000008 Genomic DNA. Translation: EAA27212.1.
RefSeq	XP_956448.1.
3D structure databases
HSSP	HSSP built from PDB template 3GRS based on UniProtKB P00390.
ModBase	Search...
Genome annotation databases
GeneID	3872613.
KEGG	ncr:NCU03339.
NMPDR	fig|5141.1.peg.530.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006322. Glut_reduct_1. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01421. gluta_reduc_1. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_NEUCR

Accession

Primary (citable) accession number: Q873E8
Secondary accession number(s): Q1K529

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)