Reviewed, UniProtKB/Swiss-Prot Q870Y1 (MPI_NEUCR)

Names and origin

Protein names

Recommended name:
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
Alternative name(s):
    Phosphomannose isomerase
      Short name=PMI
    Phosphohexomutase

Gene names

ORF Names:

103E1.170, NCU07165

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Mannose-6-phosphate isomerase		PRO_0000194245
Sites
	Active site	308	1	By similarity
	Metal binding	109	1	Zinc By similarity
	Metal binding	111	1	Zinc By similarity
	Metal binding	136	1	Zinc By similarity
	Metal binding	289	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q870Y1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D4962FC4C0AB88F3	FASTA	459	50,364
	10 20 30 40 50 60 MLPVPLIQLQ CGVNSYEWGK KGNSSRAAQF AAASLDDNEF KIDEEKPYAE LWMGTHPSNP 70 80 90 100 110 120 SKDKHTGRTL LELIQDNQAL LSPAIAERYQ NKLPFLFKVL SIQKALSIQA HPNKKLAEKL 130 140 150 160 170 180 HARDPKNYPD DNHKPEMAIA ISDFEGLCGF RPLKEIAHFL DNVPALRQLV GEDKAKAFVE 190 200 210 220 230 240 TVKRNDGNES DEATAENKKV LQSAFGALMA SSEADMTAAA DKLVQSAKSA GADFTGTAVP 250 260 270 280 290 300 SPGGAKLSEL VGRLHGQFGA DYGLFVLFFL NFVEMKAGEA IYLRADDIHA YISGDIIECM 310 320 330 340 350 360 ASSDNVVRAG FTPKFKDVDN LVNMLTYDFA PIEQQKMEPT DYTQDVKNKR EAGATLNQTA 370 380 390 400 410 420 IDSQSEIVEY NPPIEEFSVV RSLLKSQGSK VTFKPIDGPS IIICTQGQGK ISVGPKVQEI 430 440 450 KKGHVFFVGA TAECVLESED DKFETFKAFC VLDEQSNGN

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX294028 Genomic DNA. Translation: CAD71127.1. AABX02000037 Genomic DNA. Translation: EAA28154.1.
RefSeq	XP_957390.1.
3D structure databases
SMR	Q870Y1. Positions 4-451.
ModBase	Search...
Protein-protein interaction databases
STRING	Q870Y1.
Genome annotation databases
GeneID	3873528.
KEGG	ncr:NCU07165.
NMPDR	fig|5141.1.peg.5384.
Phylogenomic databases
eggNOG	fuNOG04716.
OrthoDB	EOG9SN352.
PhylomeDB	Q870Y1.
Enzyme and pathway databases
BRENDA	5.3.1.8. 266.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPI_NEUCR

Accession

Primary (citable) accession number: Q870Y1
Secondary accession number(s): Q1K5X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)