Q870G1 (LYS1_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Saccharopine dehydrogenase [NAD+, L-lysine-forming]
Short name=SDH
EC=1.5.1.7

Alternative name(s):
Lysine--2-oxoglutarate reductase

Gene names

Name:	lysA
ORF Names:	AN2873

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.
Sequence caution	The sequence AAL23682.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAA63444.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene AN2873 has been split into 2 genes: AN2873 and AN11684.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Saccharopine dehydrogenase [NAD+, L-lysine-forming]		PRO_0000199012
Sites
	Active site	205	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q870G1 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: 02C6DFBEFFCB54C4	FASTA	375	41,098
	10 20 30 40 50 60 MGSNKIWLRA ETKPAEARSA LTPTTCKALI DAGYEVTVER STQRIFDDDE FAKVGAPLVE 70 80 90 100 110 120 EGSWVKDAPK DAYILGLKEL PEDDFPLEHV HISFAHCYKE QAGWEKVLSR WPRGGGVLLD 130 140 150 160 170 180 LEFLTDDAGR RVAAFGFSAG YAGAALAVKN WAWQLTHPEG EPLAGEKPYA NQDLLIQSVK 190 200 210 220 230 240 ESLQAGQKQS GKSPKILVIG ALGRCGKGAV QLAKDVGIPE SDIIQWDMEE TKKGGPFKEI 250 260 270 280 290 300 VEDADIFVNC IYLSSKIPHF VNVESLSTPS RRLSVICDVS ADTTNPNNPI PVYNITTTFD 310 320 330 340 350 360 KPTVPVTLPN GTQGTPLSVI SIDHLPSLLP RESSEMFSEA LMPSLLQLKD RENARVWKQA 370 EDLFNQKVAT LPQTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Impact of the cross-pathway control on the regulation of lysine and penicillin biosynthesis in Aspergillus nidulans." Busch S., Bode H.B., Brakhage A.A., Braus G.H. Curr. Genet. 42:209-219(2003) [PubMed: 12589472] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed: 19146970] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY057447 Genomic DNA. Translation: AAL23682.1. Sequence problems. AACD01000051 Genomic DNA. Translation: EAA63444.1. Sequence problems. BN001306 Genomic DNA. Translation: CBF83833.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q870G1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00010216; CADANIAP00010216; CADANIAG00010216.
Phylogenomic databases
OMA	QFAHCYK.
OrthoDB	EOG408RHS.
PhylomeDB	Q870G1.
Family and domain databases
InterPro	IPR007886. AlaDH/PNT_N. IPR007698. AlaDH/PNT_NAD(H)-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
SMART	SM01002. AlaDh_PNT_C. 1 hit. SM01003. AlaDh_PNT_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS1_EMENI

Accession

Primary (citable) accession number: Q870G1
Secondary accession number(s): C8VJB0, Q5B9A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program