Q87022 (RDRP_SCVLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable RNA-directed RNA polymerase
EC=2.7.7.48

Alternative name(s):
Gag-Pol protein

Gene names

Name:

gag-pol

Organism

Saccharomyces cerevisiae virus L-A (ScV-L-A) (ScVL1) [Reference proteome]

Taxonomic identifier

11008 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Totiviridae › Totivirus

Virus host

Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]

Protein attributes

Sequence length	1505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome. Catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded from the virion into the cytoplasm where they function as mRNAs for translation of viral proteins and also as substrates for encapsidation to form new virions. Once encapsidated, the positive strand is converted to dsRNA by the RNA-directed RNA polymerase. Displays ssRNA-binding activity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Miscellaneous	One molecule or two of Gag capsid protein is replaced in the virion by Gag-Pol because the fusion protein is essential for RNA encapsidation and replication.
Sequence similarities	Belongs to the totiviridae RNA-directed RNA polymerase family.
Sequence caution	The sequence AAA50507.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAA50508.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Viral RNA replication
Coding sequence diversity	Ribosomal frameshifting
Ligand	Nucleotide-binding RNA-binding
Molecular function	Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform RNA-directed RNA polymerase (identifier: Q87022-1)

Also known as: Pol protein;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by -1 ribosomal frameshifting.

Isoform Major capsid protein (identifier: P32503-1)

Also known as: Gag protein;

The sequence of this isoform can be found in the external entry P32503.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1505	1505	Probable RNA-directed RNA polymerase		PRO_0000402407

Sequences

	Sequence		Length	Mass (Da)
	Isoform RNA-directed RNA polymerase (Pol protein) [UniParc]. Last modified November 30, 2010. Version 2. Checksum: B900A831DD49F72A	FASTA	1,505	170,480
	10 20 30 40 50 60 MLRFVTKNSQ DKSSDLFSIC SDRGTFVAHN RVRTDFKFDN LVFNRVYGVS QKFTLVGNPT 70 80 90 100 110 120 VCFNEGSSYL EGIAKKYLTL DGGLAIDNVL NELRSTCGIP GNAVASHAYN ITSWRWYDNH 130 140 150 160 170 180 VALLMNMLRA YHLQVLTEQG QYSAGDIPMY HDGHVKIKLP VTIDDTAGPT QFAWPSDRST 190 200 210 220 230 240 DSYPDWAQFS ESFPSIDVPY LDVRPLTVTE VNFVLMMMSK WHRRTNLAID YEAPQLADKF 250 260 270 280 290 300 AYRHALTVQD ADEWIEGDRT DDQFRPPSSK VMLSALRKYV NHNRLYNQFY TAAQLLAQIM 310 320 330 340 350 360 MKPVPNCAEG YAWLMHDALV NIPKFGSIRG RYPFLLSGDA ALIQATALED WSAIMAKPEL 370 380 390 400 410 420 VFTYAMQVSV ALNTGLYLRR VKKTGFGTTI DDSYEDGAFL QPETFVQAAL ACCTGQDAPL 430 440 450 460 470 480 NGMSDVYVTY PDLLEFDAVT QVPITVIEPA GYNIVDDHLV VVGVPVACSP YMIFPVAAFD 490 500 510 520 530 540 TANPYCGNFV IKAANKYLRK GAVYDKLEAW KLAWALRVAG YDTHFKVYGD THGLTKFYAD 550 560 570 580 590 600 NGDTWTHIPE FVTDGDVMEV FVTAIERRAR HFVELPRLNS PAFFRSVEVS TTIYDTHVQA 610 620 630 640 650 660 GAHAVYHASR INLDYVKPVS TGIQVINAGE LKNYWGSVRR TQQGLRSGRS YDASCNAYRR 670 680 690 700 710 720 TYSWRCPRRV DRTGGQCFSR VNVIEPSHGP RPTRYILQEP GTYPAWIRFR NRVQAVSRQK 730 740 750 760 770 780 ATHFLFDIVP AAVISDFTTS DTSSFAYKSH TYAVNVTALR FSDTYALYVQ TDTNMTILSP 790 800 810 820 830 840 AARRQASATY SQVAGFCYNT PTVMDSLANI LDVDRNIRPK HFKGLRLYTR SKVTAQHHTH 850 860 870 880 890 900 LRPDELVEAA AKVSPRRKYY LMCVVELLAN LQVDLEAAVA TILAYVLTLS EKFVPIFLDS 910 920 930 940 950 960 RAIWVGEPGP DALTARLKAS SGQIKSIHTA DYEPLTELFE LAVLMNRGVG HVSWQAEKDH 970 980 990 1000 1010 1020 RLNPDVAVVD QARLYSCVRD MFEGSKQTYK YPFMTWDDYT ANRWEWVPGG SVHSQYEEDN 1030 1040 1050 1060 1070 1080 DYIYPGQYTR NKFITVNKMP KHKISRMIAS PPEVRAWTST KYEWGKQRAI YGTDLRSTLI 1090 1100 1110 1120 1130 1140 TNFAMFRCED VLTHKFPVGD QAEAAKVHKR VNMMLDGASS FCFDYDDFNS QHSIASMYTV 1150 1160 1170 1180 1190 1200 LCAFRDTFSR NMSDEQAEAM NWVCESVRHM WVLDPDTKEW YRLQGTLLSG WRLTTFMNTV 1210 1220 1230 1240 1250 1260 LNWAYMKLAG VFDLDDVQDS VHNGDDVMIS LNRVSTAVRI MDAMHRINAR AQPAKCNLFS 1270 1280 1290 1300 1310 1320 ISEFLRVEHG MSGGDGLGAQ YLSRSCATLV HSRIESNEPL SVVRVMEADQ ARLRDLANRT 1330 1340 1350 1360 1370 1380 RVQSAVTAIK EQLDKRVTKI FGVGDDVVRD IHTAHRVCGG ISTDTWAPVE TKIITDNEAY 1390 1400 1410 1420 1430 1440 EIPYEIDDPS FWPGVNDYAY KVWKNFGERL EFNKIKDAVA RGSRSTIALK RKARITSKKN 1450 1460 1470 1480 1490 1500 EFANKSEWER TMYKAYKGLA VSYYANLSKF MSIPPMANIE FGQARYAMQA ALDSSDPLRA LQVIL
	Isoform Major capsid protein (Gag protein) [UniParc]. See P32503.		–	–

References

[1]	"The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames." Icho T., Wickner R.B. J. Biol. Chem. 264:6716-6723(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04692 Genomic RNA. Translation: AAA50507.1. Sequence problems. J04692 Genomic RNA. Translation: AAA50508.1. Sequence problems.
RefSeq	NP_620493.1. NC_003745.1. NP_620495.1. NC_003745.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	940476. 940478.
Family and domain databases
InterPro	IPR015302. Major_coat_LA-virus. IPR001795. RNA-dir_pol_luteovirus. [Graphical view]
Pfam	PF09220. LA-virus_coat. 1 hit. PF02123. RdRP_4. 1 hit. [Graphical view]
SUPFAM	SSF82856. Major_coat_LA-virus. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RDRP_SCVLA

Accession

Primary (citable) accession number: Q87022
Secondary accession number(s): Q87023

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	April 3, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program