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Q87013 (REP6_SCSVF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Initiates and terminates the replication only of its own subviral DNA molecule. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Cofactor

Divalent metal cations, possibly magnesium or manganese By similarity.

Subunit structure

Homooligomer Potential. Rep binds to repeated DNA motifs (iterons) By similarity.

Subcellular location

Host nucleus Potential.

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR By similarity.

Miscellaneous

The genome of nanoviruses is composed of six to eight segments. In addition, some isolates contain subviral DNAs.

Sequence similarities

Belongs to the nanoviridea/circoviridae replication-associated protein family.

Caution

This protein is encoded by a subviral DNA that is not present in all isolates of the virus.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Para-Rep C6
PRO_0000378525

Regions

Nucleotide binding175 – 1839ATP By similarity
Motif11 – 144RCR-1 By similarity
Motif42 – 476RCR-2 By similarity
Motif52 – 7423Nuclear localization signal Potential
Motif82 – 854RCR-3 By similarity
Motif99 – 1057Nuclear localization signal Potential

Sites

Active site821For DNA cleavage activity By similarity
Metal binding361Divalent metal cation Potential
Metal binding421Divalent metal cation Potential
Metal binding871Divalent metal cation Potential

Sequences

Sequence LengthMass (Da)Tools
Q87013 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EDC1315985477504

FASTA28533,500
        10         20         30         40         50         60 
MPTRQSTSWV FTLNFEGEIP ILPFNESVQY ACWQHERVGH DHLQGFIQFK SRNTTLRQAK 

        70         80         90        100        110        120 
YIFNGLNPHL EIARDVEKAQ LYAMKEDSRV AGPWEYGLFI KRGSHKRKLM ERFEEDGEEM 

       130        140        150        160        170        180 
KIADPSLYRR CLSRKMAEEQ RCSSEWNYDL RPWQEEVMHL LEEEPDYRTI IWVYGPAGNE 

       190        200        210        220        230        240 
GKSTFARHLS LKDGWGYLPG GKTQDMMHLV TAEPKNNWVF DIPRVSSEYV NYGVIEQVKN 

       250        260        270        280 
RVMVNTKYEP CVMRDDNHPV HVIVFANVLP DLGKLSEDRI KLIRC 

« Hide

References

[1]"Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses."
Boevink P.C., Chu P.W.G., Keese P.K.
Virology 207:354-361(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U16735 Genomic DNA. Translation: AAA68022.1.

3D structure databases

ProteinModelPortalQ87013.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR003365. Viral_rep_N.
[Graphical view]
PfamPF00910. RNA_helicase. 1 hit.
PF02407. Viral_Rep. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameREP6_SCSVF
AccessionPrimary (citable) accession number: Q87013
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families