Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycoprotein hormone beta-5

Gene

GPHB5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the thyroid. Binds and activates THSR, leading to increased cAMP production.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hormone

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein hormone beta-5
Alternative name(s):
Thyrostimulin subunit beta
Gene namesi
Name:GPHB5
Synonyms:GPB5, ZLUT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:18055. GPHB5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134872371.

Polymorphism and mutation databases

BioMutaiGPHB5.
DMDMi48474403.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 130106Glycoprotein hormone beta-5PRO_0000011761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 84Sequence analysis
Disulfide bondi50 ↔ 99Sequence analysis
Disulfide bondi60 ↔ 115Sequence analysis
Disulfide bondi64 ↔ 117Sequence analysis
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence analysis
Disulfide bondi120 ↔ 127Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ86YW7.

Expressioni

Tissue specificityi

Highly expressed in brain and at low levels in pituitary. Also found in retina, testis and skin but not in pancreas, parotid, kidney, stomach, liver, colon, small intestine, thyroid, brain or adrenal gland. In pituitary, colocalizes with ACTH, suggesting that it is located in corticotrophs.2 Publications

Gene expression databases

CleanExiHS_GPHB5.
ExpressionAtlasiQ86YW7. baseline and differential.
GenevisibleiQ86YW7. HS.

Interactioni

Subunit structurei

Heterodimer with GPHA2.

Structurei

3D structure databases

ProteinModelPortaliQ86YW7.
SMRiQ86YW7. Positions 42-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00730000111179.
HOGENOMiHOG000060217.
HOVERGENiHBG006698.
InParanoidiQ86YW7.
OMAiAKKPGCK.
PhylomeDBiQ86YW7.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR006208. Glyco_hormone_CN.
IPR001545. Gonadotropin_bsu.
[Graphical view]
PANTHERiPTHR11515. PTHR11515. 1 hit.
PfamiPF00007. Cys_knot. 1 hit.
[Graphical view]
SMARTiSM00068. GHB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86YW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAFLFLGP MALLLLAGYG CVLGASSGNL RTFVGCAVRE FTFLAKKPGC
60 70 80 90 100
RGLRITTDAC WGRCETWEKP ILEPPYIEAH HRVCTYNETK QVTVKLPNCA
110 120 130
PGVDPFYTYP VAIRCDCGAC STATTECETI
Length:130
Mass (Da):14,232
Last modified:June 1, 2003 - v1
Checksum:i2C6C3BC0AA6725B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681E → Q in AAM53261 (PubMed:12045258).Curated
Sequence conflicti68 – 681E → Q in AAH69113 (PubMed:15489334).Curated
Sequence conflicti112 – 12211AIRCDCGACST → PSAVTAEPAPL in AAM53261 (PubMed:12045258).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403430 mRNA. Translation: AAM53261.1.
AF467770 mRNA. Translation: AAO33390.1.
AL118555 Genomic DNA. No translation available.
BC069113 mRNA. Translation: AAH69113.1.
CCDSiCCDS73643.1.
RefSeqiNP_660154.3. NM_145171.3.
UniGeneiHs.375028.

Genome annotation databases

EnsembliENST00000314140; ENSP00000479431; ENSG00000179600.
ENST00000621500; ENSP00000478993; ENSG00000179600.
GeneIDi122876.
KEGGihsa:122876.
UCSCiuc032bdx.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403430 mRNA. Translation: AAM53261.1.
AF467770 mRNA. Translation: AAO33390.1.
AL118555 Genomic DNA. No translation available.
BC069113 mRNA. Translation: AAH69113.1.
CCDSiCCDS73643.1.
RefSeqiNP_660154.3. NM_145171.3.
UniGeneiHs.375028.

3D structure databases

ProteinModelPortaliQ86YW7.
SMRiQ86YW7. Positions 42-127.
ModBaseiSearch...
MobiDBiSearch...

Polymorphism and mutation databases

BioMutaiGPHB5.
DMDMi48474403.

Proteomic databases

PRIDEiQ86YW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000314140; ENSP00000479431; ENSG00000179600.
ENST00000621500; ENSP00000478993; ENSG00000179600.
GeneIDi122876.
KEGGihsa:122876.
UCSCiuc032bdx.1. human.

Organism-specific databases

CTDi122876.
GeneCardsiGPHB5.
H-InvDBHIX0037679.
HGNCiHGNC:18055. GPHB5.
MIMi609652. gene.
neXtProtiNX_Q86YW7.
PharmGKBiPA134872371.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00730000111179.
HOGENOMiHOG000060217.
HOVERGENiHBG006698.
InParanoidiQ86YW7.
OMAiAKKPGCK.
PhylomeDBiQ86YW7.

Miscellaneous databases

GeneWikiiGPHB5.
GenomeRNAii122876.
PROiQ86YW7.
SOURCEiSearch...

Gene expression databases

CleanExiHS_GPHB5.
ExpressionAtlasiQ86YW7. baseline and differential.
GenevisibleiQ86YW7. HS.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR006208. Glyco_hormone_CN.
IPR001545. Gonadotropin_bsu.
[Graphical view]
PANTHERiPTHR11515. PTHR11515. 1 hit.
PfamiPF00007. Cys_knot. 1 hit.
[Graphical view]
SMARTiSM00068. GHB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Thyrostimulin, a heterodimer of two new human glycoprotein hormone subunits, activates the thyroid-stimulating hormone receptor."
    Nakabayashi K., Matsumi H., Bhalla A., Bae J., Mosselman S., Hsu S.Y., Hsueh A.J.W.
    J. Clin. Invest. 109:1445-1452(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH GPHA2, GLYCOSYLATION.
  2. "A novel glycoprotein hormone beta subunit."
    Holloway J.L., O'Hogan S.L., Tackett M., Taft D., Thayer E.C., Webster P.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5."
    Hsu S.Y., Nakabayashi K., Bhalla A.
    Mol. Endocrinol. 16:1538-1551(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "A glycoprotein hormone expressed in corticotrophs exhibits unique binding properties on thyroid-stimulating hormone receptor."
    Okada S.L., Ellsworth J.L., Durnam D.M., Haugen H.S., Holloway J.L., Kelley M.L., Lewis K.E., Ren H., Sheppard P.O., Storey H.M., Waggie K.S., Wolf A.C., Yao L.Y., Webster P.J.
    Mol. Endocrinol. 20:414-425(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGPHB5_HUMAN
AccessioniPrimary (citable) accession number: Q86YW7
Secondary accession number(s): Q6NTD0, Q8NFW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.