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Protein

Glycoprotein hormone beta-5

Gene

GPHB5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a heterodimeric glycoprotein hormone with GPHA2 able to bind and activate the thyroid-stimulating hormone receptor (TSHR), leading to increased cAMP production. Plays a central role in controlling thyroid cell metabolism.2 Publications

GO - Molecular functioni

  • hormone activity Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • thyrotropin-releasing hormone receptor binding Source: UniProtKB

GO - Biological processi

  • cell-cell signaling Source: GO_Central
  • cell surface receptor signaling pathway Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: GO_Central
  • hormone-mediated signaling pathway Source: GO_Central
  • positive regulation of cAMP biosynthetic process Source: UniProtKB
  • regulation of thyroid hormone mediated signaling pathway Source: GO_Central

Keywordsi

Molecular functionHormone

Enzyme and pathway databases

ReactomeiR-HSA-375281. Hormone ligand-binding receptors.
R-HSA-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein hormone beta-5
Alternative name(s):
Thyrostimulin subunit beta
Gene namesi
Name:GPHB5
Synonyms:GPB5, ZLUT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000179600.3.
HGNCiHGNC:18055. GPHB5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi122876.
OpenTargetsiENSG00000179600.
PharmGKBiPA134872371.

Polymorphism and mutation databases

BioMutaiGPHB5.
DMDMi48474403.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001176125 – 130Glycoprotein hormone beta-5Add BLAST106

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 84Sequence analysis
Disulfide bondi50 ↔ 99Sequence analysis
Disulfide bondi60 ↔ 115Sequence analysis
Disulfide bondi64 ↔ 117Sequence analysis
Glycosylationi87N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi120 ↔ 127Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ86YW7.

Expressioni

Tissue specificityi

Highly expressed in brain and at low levels in pituitary. Also found in retina, testis and skin but not in pancreas, parotid, kidney, stomach, liver, colon, small intestine, thyroid, brain or adrenal gland. In pituitary, colocalizes with ACTH, suggesting that it is located in corticotrophs.2 Publications

Gene expression databases

BgeeiENSG00000179600.
CleanExiHS_GPHB5.
ExpressionAtlasiQ86YW7. baseline and differential.
GenevisibleiQ86YW7. HS.

Interactioni

Subunit structurei

Heterodimer with GPHA2; this heterodimer interacts with thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP production.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GPHA2Q96T913EBI-11659720,EBI-11659696

GO - Molecular functioni

  • hormone activity Source: UniProtKB-KW
  • protein heterodimerization activity Source: UniProtKB
  • thyrotropin-releasing hormone receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ86YW7. 4 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ86YW7.
SMRiQ86YW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00730000111179.
HOGENOMiHOG000060217.
HOVERGENiHBG006698.
InParanoidiQ86YW7.
OMAiLAKKPGC.
OrthoDBiEOG091G0XFH.
PhylomeDBiQ86YW7.

Family and domain databases

CDDicd00069. GHB_like. 1 hit.
Gene3Di2.10.90.10. 1 hit.
InterProiView protein in InterPro
IPR029034. Cystine-knot_cytokine.
IPR006208. Glyco_hormone_CN.
IPR001545. Gonadotropin_bsu.
PANTHERiPTHR11515. PTHR11515. 1 hit.
PfamiView protein in Pfam
PF00007. Cys_knot. 1 hit.
SMARTiView protein in SMART
SM00068. GHB. 1 hit.
SUPFAMiSSF57501. SSF57501. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86YW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLAFLFLGP MALLLLAGYG CVLGASSGNL RTFVGCAVRE FTFLAKKPGC
60 70 80 90 100
RGLRITTDAC WGRCETWEKP ILEPPYIEAH HRVCTYNETK QVTVKLPNCA
110 120 130
PGVDPFYTYP VAIRCDCGAC STATTECETI
Length:130
Mass (Da):14,232
Last modified:June 1, 2003 - v1
Checksum:i2C6C3BC0AA6725B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68E → Q in AAM53261 (PubMed:12045258).Curated1
Sequence conflicti68E → Q in AAH69113 (PubMed:15489334).Curated1
Sequence conflicti112 – 122AIRCDCGACST → PSAVTAEPAPL in AAM53261 (PubMed:12045258).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403430 mRNA. Translation: AAM53261.1.
AF467770 mRNA. Translation: AAO33390.1.
AL118555 Genomic DNA. No translation available.
BC069113 mRNA. Translation: AAH69113.1.
CCDSiCCDS73643.1.
RefSeqiNP_660154.3. NM_145171.3.
UniGeneiHs.375028.

Genome annotation databases

EnsembliENST00000314140; ENSP00000479431; ENSG00000179600.
ENST00000621500; ENSP00000478993; ENSG00000179600.
GeneIDi122876.
KEGGihsa:122876.
UCSCiuc032bdx.1. human.

Similar proteinsi

Entry informationi

Entry nameiGPHB5_HUMAN
AccessioniPrimary (citable) accession number: Q86YW7
Secondary accession number(s): Q6NTD0, Q8NFW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 1, 2003
Last modified: October 25, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families