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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarityCurated3 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701PROSITE-ProRule annotationBy similarity
Active sitei215 – 2151PROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_150312. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-11 Publication
Short name:
PLC-zeta-11 Publication
Testis-development protein NYD-SP27
Gene namesi
Name:PLCZ1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:19218. PLCZ1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmperinuclear region By similarity

  • Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134875646.

Polymorphism and mutation databases

DMDMi74714209.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6086081-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347244Add
BLAST

Proteomic databases

PaxDbiQ86YW0.
PRIDEiQ86YW0.

Expressioni

Tissue specificityi

Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.2 Publications

Gene expression databases

BgeeiQ86YW0.
CleanExiHS_PLCZ1.
ExpressionAtlasiQ86YW0. baseline and differential.
GenevestigatoriQ86YW0.

Interactioni

Subunit structurei

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

Protein-protein interaction databases

BioGridi124625. 5 interactions.
STRINGi9606.ENSP00000266505.

Structurei

3D structure databases

ProteinModelPortaliQ86YW0.
SMRiQ86YW0. Positions 7-604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 7036EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini155 – 299145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini349 – 465117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini469 – 572104C2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ86YW0.
KOiK05861.
OMAiYRIITHR.
OrthoDBiEOG7V49XT.
PhylomeDBiQ86YW0.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 13 Publications (identifier: Q86YW0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMRWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KQIFKDNDRL
60 70 80 90 100
KQGRITIEEF RAIYRIITHR EEIIEIFNTY SENRKILLAS NLAQFLTQEQ
110 120 130 140 150
YAAEMSKAIA FEIIQKYEPI EEVRKAHQMS LEGFTRYMDS RECLLFKNEC
160 170 180 190 200
RKVYQDMTHP LNDYFISSSH NTYLVSDQLL GPSDLWGYVS ALVKGCRCLE
210 220 230 240 250
IDCWDGAQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFMTS DYPVVLSLEN
260 270 280 290 300
HCSTAQQEVM ADNLQATFGE SLLSDMLDDF PDTLPSPEAL KFKILVKNKK
310 320 330 340 350
IGTLKETHER KGSDKRGDNQ DKETGVKKLP GVMLFKKKKT RKLKIALALS
360 370 380 390 400
DLVIYTKAEK FKSFQHSRLY QQFNENNSIG ETQARKLSKL RVHEFIFHTR
410 420 430 440 450
KFITRIYPKA TRADSSNFNP QEFWNIGCQM VALNFQTPGL PMDLQNGKFL
460 470 480 490 500
DNGGSGYILK PHFLRESKSY FNPSNIKEGM PITLTIRLIS GIQLPLTHSS
510 520 530 540 550
SNKGDSLVII EVFGVPNDQM KQQTRVIKKN AFSPRWNETF TFIIHVPELA
560 570 580 590 600
LIRFVVEGQG LIAGNEFLGQ YTLPLLCMNK GYRRIPLFSR MGESLEPASL

FVYVWYVR
Length:608
Mass (Da):70,411
Last modified:June 1, 2003 - v1
Checksum:i540A81A2DDB9537B
GO
Isoform 21 Publication (identifier: Q86YW0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     46-238: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:415
Mass (Da):47,758
Checksum:i68B6337B885663DE
GO
Isoform 3 (identifier: Q86YW0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Show »
Length:504
Mass (Da):57,804
Checksum:i671C2692852FED44
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti294 – 2941I → V in AAK61372 (PubMed:14697805).Curated
Sequence conflicti489 – 4891I → T in AAK61372 (PubMed:14697805).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti500 – 5001S → L.
Corresponds to variant rs10505830 [ dbSNP | Ensembl ].
VAR_050542

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 104104Missing in isoform 3. 1 PublicationVSP_035076Add
BLAST
Alternative sequencei46 – 238193Missing in isoform 2. 1 PublicationVSP_052862Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532185 mRNA. Translation: AAN71895.1.
AY035866 mRNA. Translation: AAK61372.1.
AK292279 mRNA. Translation: BAF84968.1.
BC125067 mRNA. Translation: AAI25068.1.
CCDSiCCDS8680.1. [Q86YW0-1]
RefSeqiNP_149114.2. NM_033123.3. [Q86YW0-1]
UniGeneiHs.743832.
Hs.97542.

Genome annotation databases

EnsembliENST00000266505; ENSP00000266505; ENSG00000139151. [Q86YW0-1]
ENST00000539875; ENSP00000445026; ENSG00000139151. [Q86YW0-2]
GeneIDi89869.
KEGGihsa:89869.
UCSCiuc001rdv.5. human. [Q86YW0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532185 mRNA. Translation: AAN71895.1.
AY035866 mRNA. Translation: AAK61372.1.
AK292279 mRNA. Translation: BAF84968.1.
BC125067 mRNA. Translation: AAI25068.1.
CCDSiCCDS8680.1. [Q86YW0-1]
RefSeqiNP_149114.2. NM_033123.3. [Q86YW0-1]
UniGeneiHs.743832.
Hs.97542.

3D structure databases

ProteinModelPortaliQ86YW0.
SMRiQ86YW0. Positions 7-604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124625. 5 interactions.
STRINGi9606.ENSP00000266505.

Polymorphism and mutation databases

DMDMi74714209.

Proteomic databases

PaxDbiQ86YW0.
PRIDEiQ86YW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000266505; ENSP00000266505; ENSG00000139151. [Q86YW0-1]
ENST00000539875; ENSP00000445026; ENSG00000139151. [Q86YW0-2]
GeneIDi89869.
KEGGihsa:89869.
UCSCiuc001rdv.5. human. [Q86YW0-1]

Organism-specific databases

CTDi89869.
GeneCardsiGC12M018736.
H-InvDBHIX0010470.
HGNCiHGNC:19218. PLCZ1.
MIMi608075. gene.
neXtProtiNX_Q86YW0.
PharmGKBiPA134875646.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ86YW0.
KOiK05861.
OMAiYRIITHR.
OrthoDBiEOG7V49XT.
PhylomeDBiQ86YW0.
TreeFamiTF313216.

Enzyme and pathway databases

ReactomeiREACT_150312. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

ChiTaRSiPLCZ1. human.
GenomeRNAii89869.
NextBioi76366.
PROiQ86YW0.
SOURCEiSearch...

Gene expression databases

BgeeiQ86YW0.
CleanExiHS_PLCZ1.
ExpressionAtlasiQ86YW0. baseline and differential.
GenevestigatoriQ86YW0.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes."
    Cox L.J., Larman M.G., Saunders C.M., Hashimoto K., Swann K., Lai F.A.
    Reproduction 124:611-623(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: TestisImported.
  2. "Rescue of defective pancreatic secretion in cystic-fibrosis cells by suppression of a novel isoform of phospholipase C."
    Zhu H., Zhu J.X., Lo P.S., Li J., Leung K.M., Rowlands D.K., Tsang L.L., Yu M.K., Jiang J.L., Lam S.Y., Chung Y.W., Zhou Z., Sha J., Chang Chan H.
    Lancet 362:2059-2065(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
    Tissue: TestisImported.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: TestisImported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "Phospholipase Czeta causes Ca2+ oscillations and parthenogenetic activation of human oocytes."
    Rogers N.T., Hobson E., Pickering S., Lai F.A., Braude P., Swann K.
    Reproduction 128:697-702(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPLCZ1_HUMAN
AccessioniPrimary (citable) accession number: Q86YW0
Secondary accession number(s): Q08AQ7, Q96J70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.