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Q86YW0

- PLCZ1_HUMAN

UniProt

Q86YW0 - PLCZ1_HUMAN

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity3 PublicationsCurated

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei170 – 1701By similarityPROSITE-ProRule annotation
    Active sitei215 – 2151By similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. calcium ion transport Source: Ensembl
    2. egg activation Source: Ensembl
    3. inositol phosphate metabolic process Source: Reactome
    4. intracellular signal transduction Source: InterPro
    5. lipid catabolic process Source: UniProtKB-KW
    6. multicellular organismal development Source: UniProtKB-KW
    7. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Transducer

    Keywords - Biological processi

    Fertilization, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_150312. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-zeta-1
    Phospholipase C-zeta-11 Publication
    Short name:
    PLC-zeta-11 Publication
    Testis-development protein NYD-SP27
    Gene namesi
    Name:PLCZ1Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:19218. PLCZ1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmperinuclear region By similarity
    Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. nucleus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134875646.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6086081-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347244Add
    BLAST

    Proteomic databases

    PaxDbiQ86YW0.
    PRIDEiQ86YW0.

    Expressioni

    Tissue specificityi

    Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.2 Publications

    Gene expression databases

    ArrayExpressiQ86YW0.
    BgeeiQ86YW0.
    CleanExiHS_PLCZ1.
    GenevestigatoriQ86YW0.

    Interactioni

    Subunit structurei

    Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

    Protein-protein interaction databases

    STRINGi9606.ENSP00000266505.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86YW0.
    SMRiQ86YW0. Positions 8-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 7036EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini155 – 299145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 465117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini469 – 572104C2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000006871.
    HOVERGENiHBG053610.
    InParanoidiQ86YW0.
    KOiK05861.
    OMAiYRIITHR.
    OrthoDBiEOG7V49XT.
    PhylomeDBiQ86YW0.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 13 Publications (identifier: Q86YW0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEMRWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KQIFKDNDRL    50
    KQGRITIEEF RAIYRIITHR EEIIEIFNTY SENRKILLAS NLAQFLTQEQ 100
    YAAEMSKAIA FEIIQKYEPI EEVRKAHQMS LEGFTRYMDS RECLLFKNEC 150
    RKVYQDMTHP LNDYFISSSH NTYLVSDQLL GPSDLWGYVS ALVKGCRCLE 200
    IDCWDGAQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFMTS DYPVVLSLEN 250
    HCSTAQQEVM ADNLQATFGE SLLSDMLDDF PDTLPSPEAL KFKILVKNKK 300
    IGTLKETHER KGSDKRGDNQ DKETGVKKLP GVMLFKKKKT RKLKIALALS 350
    DLVIYTKAEK FKSFQHSRLY QQFNENNSIG ETQARKLSKL RVHEFIFHTR 400
    KFITRIYPKA TRADSSNFNP QEFWNIGCQM VALNFQTPGL PMDLQNGKFL 450
    DNGGSGYILK PHFLRESKSY FNPSNIKEGM PITLTIRLIS GIQLPLTHSS 500
    SNKGDSLVII EVFGVPNDQM KQQTRVIKKN AFSPRWNETF TFIIHVPELA 550
    LIRFVVEGQG LIAGNEFLGQ YTLPLLCMNK GYRRIPLFSR MGESLEPASL 600
    FVYVWYVR 608
    Length:608
    Mass (Da):70,411
    Last modified:June 1, 2003 - v1
    Checksum:i540A81A2DDB9537B
    GO
    Isoform 21 Publication (identifier: Q86YW0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         46-238: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:415
    Mass (Da):47,758
    Checksum:i68B6337B885663DE
    GO
    Isoform 3 (identifier: Q86YW0-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-104: Missing.

    Show »
    Length:504
    Mass (Da):57,804
    Checksum:i671C2692852FED44
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti294 – 2941I → V in AAK61372. (PubMed:14697805)Curated
    Sequence conflicti489 – 4891I → T in AAK61372. (PubMed:14697805)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti500 – 5001S → L.
    Corresponds to variant rs10505830 [ dbSNP | Ensembl ].
    VAR_050542

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 104104Missing in isoform 3. 1 PublicationVSP_035076Add
    BLAST
    Alternative sequencei46 – 238193Missing in isoform 2. 1 PublicationVSP_052862Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF532185 mRNA. Translation: AAN71895.1.
    AY035866 mRNA. Translation: AAK61372.1.
    AK292279 mRNA. Translation: BAF84968.1.
    BC125067 mRNA. Translation: AAI25068.1.
    CCDSiCCDS8680.1. [Q86YW0-1]
    RefSeqiNP_149114.2. NM_033123.3. [Q86YW0-1]
    UniGeneiHs.743832.
    Hs.97542.

    Genome annotation databases

    EnsembliENST00000266505; ENSP00000266505; ENSG00000139151. [Q86YW0-1]
    ENST00000539875; ENSP00000445026; ENSG00000139151. [Q86YW0-2]
    GeneIDi89869.
    KEGGihsa:89869.
    UCSCiuc001rdv.5. human. [Q86YW0-1]

    Polymorphism databases

    DMDMi74714209.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF532185 mRNA. Translation: AAN71895.1 .
    AY035866 mRNA. Translation: AAK61372.1 .
    AK292279 mRNA. Translation: BAF84968.1 .
    BC125067 mRNA. Translation: AAI25068.1 .
    CCDSi CCDS8680.1. [Q86YW0-1 ]
    RefSeqi NP_149114.2. NM_033123.3. [Q86YW0-1 ]
    UniGenei Hs.743832.
    Hs.97542.

    3D structure databases

    ProteinModelPortali Q86YW0.
    SMRi Q86YW0. Positions 8-604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000266505.

    Polymorphism databases

    DMDMi 74714209.

    Proteomic databases

    PaxDbi Q86YW0.
    PRIDEi Q86YW0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000266505 ; ENSP00000266505 ; ENSG00000139151 . [Q86YW0-1 ]
    ENST00000539875 ; ENSP00000445026 ; ENSG00000139151 . [Q86YW0-2 ]
    GeneIDi 89869.
    KEGGi hsa:89869.
    UCSCi uc001rdv.5. human. [Q86YW0-1 ]

    Organism-specific databases

    CTDi 89869.
    GeneCardsi GC12M018736.
    H-InvDB HIX0010470.
    HGNCi HGNC:19218. PLCZ1.
    MIMi 608075. gene.
    neXtProti NX_Q86YW0.
    PharmGKBi PA134875646.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000006871.
    HOVERGENi HBG053610.
    InParanoidi Q86YW0.
    KOi K05861.
    OMAi YRIITHR.
    OrthoDBi EOG7V49XT.
    PhylomeDBi Q86YW0.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_150312. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    ChiTaRSi PLCZ1. human.
    GenomeRNAii 89869.
    NextBioi 76366.
    PROi Q86YW0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86YW0.
    Bgeei Q86YW0.
    CleanExi HS_PLCZ1.
    Genevestigatori Q86YW0.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes."
      Cox L.J., Larman M.G., Saunders C.M., Hashimoto K., Swann K., Lai F.A.
      Reproduction 124:611-623(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: TestisImported.
    2. "Rescue of defective pancreatic secretion in cystic-fibrosis cells by suppression of a novel isoform of phospholipase C."
      Zhu H., Zhu J.X., Lo P.S., Li J., Leung K.M., Rowlands D.K., Tsang L.L., Yu M.K., Jiang J.L., Lam S.Y., Chung Y.W., Zhou Z., Sha J., Chang Chan H.
      Lancet 362:2059-2065(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
      Tissue: TestisImported.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: TestisImported.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    5. "Phospholipase Czeta causes Ca2+ oscillations and parthenogenetic activation of human oocytes."
      Rogers N.T., Hobson E., Pickering S., Lai F.A., Braude P., Swann K.
      Reproduction 128:697-702(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPLCZ1_HUMAN
    AccessioniPrimary (citable) accession number: Q86YW0
    Secondary accession number(s): Q08AQ7, Q96J70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3