Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin light chain kinase family member 4

Gene

MYLK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135ATPPROSITE-ProRule annotation1
Active sitei227Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi112 – 120ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS14121-MONOMER.
SignaLinkiQ86YV6.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase family member 4 (EC:2.7.11.1)
Alternative name(s):
Sugen kinase 85
Short name:
SgK085
Gene namesi
Name:MYLK4
Synonyms:SGK085
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:27972. MYLK4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi340156.
OpenTargetsiENSG00000145949.
PharmGKBiPA162396406.

Chemistry databases

ChEMBLiCHEMBL5426.
GuidetoPHARMACOLOGYi2111.

Polymorphism and mutation databases

BioMutaiMYLK4.
DMDMi118573873.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002610301 – 388Myosin light chain kinase family member 4Add BLAST388

Proteomic databases

MaxQBiQ86YV6.
PaxDbiQ86YV6.
PeptideAtlasiQ86YV6.
PRIDEiQ86YV6.

PTM databases

PhosphoSitePlusiQ86YV6.

Expressioni

Gene expression databases

BgeeiENSG00000145949.
CleanExiHS_MYLK4.
GenevisibleiQ86YV6. HS.

Organism-specific databases

HPAiHPA015860.

Interactioni

Protein-protein interaction databases

BioGridi131005. 8 interactors.
IntActiQ86YV6. 2 interactors.
STRINGi9606.ENSP00000274643.

Chemistry databases

BindingDBiQ86YV6.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi96 – 98Combined sources3
Helixi100 – 102Combined sources3
Beta strandi104 – 111Combined sources8
Beta strandi120 – 125Combined sources6
Turni126 – 128Combined sources3
Beta strandi131 – 138Combined sources8
Helixi142 – 155Combined sources14
Beta strandi166 – 171Combined sources6
Beta strandi173 – 181Combined sources9
Helixi189 – 194Combined sources6
Helixi196 – 198Combined sources3
Helixi201 – 220Combined sources20
Helixi230 – 232Combined sources3
Beta strandi233 – 237Combined sources5
Turni238 – 241Combined sources4
Beta strandi242 – 245Combined sources4
Helixi272 – 275Combined sources4
Helixi282 – 298Combined sources17
Helixi308 – 317Combined sources10
Helixi325 – 327Combined sources3
Helixi332 – 339Combined sources8
Helixi346 – 348Combined sources3
Helixi352 – 357Combined sources6
Helixi359 – 362Combined sources4
Helixi364 – 372Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X4FX-ray2.67A/B40-388[»]
ProteinModelPortaliQ86YV6.
SMRiQ86YV6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86YV6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 361Protein kinasePROSITE-ProRule annotationAdd BLAST256

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118877.
HOGENOMiHOG000233016.
HOVERGENiHBG095569.
InParanoidiQ86YV6.
KOiK00907.
OMAiFYTVSKT.
OrthoDBiEOG091G005W.
PhylomeDBiQ86YV6.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YV6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKVKRLEEF NTCYNSNQLE KMAFFQCREE VEKVKCFLEK NSGDQDSRSG
60 70 80 90 100
HNEAKEVWSN ADLTERMPVK SKRTSALAVD IPAPPAPFDH RIVTAKQGAV
110 120 130 140 150
NSFYTVSKTE ILGGGRFGQV HKCEETATGL KLAAKIIKTR GMKDKEEVKN
160 170 180 190 200
EISVMNQLDH ANLIQLYDAF ESKNDIVLVM EYVDGGELFD RIIDESYNLT
210 220 230 240 250
ELDTILFMKQ ICEGIRHMHQ MYILHLDLKP ENILCVNRDA KQIKIIDFGL
260 270 280 290 300
ARRYKPREKL KVNFGTPEFL APEVVNYDFV SFPTDMWSVG VIAYMLLSGL
310 320 330 340 350
SPFLGDNDAE TLNNILACRW DLEDEEFQDI SEEAKEFISK LLIKEKSWRI
360 370 380
SASEALKHPW LSDHKLHSRL NAQKKKNRGS DAQDFVTK
Note: No experimental confirmation available.
Length:388
Mass (Da):44,508
Last modified:November 28, 2006 - v2
Checksum:iEF710F6145DFC3C9
GO
Isoform 2 (identifier: Q86YV6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     234-252: LCVNRDAKQIKIIDFGLAR → QCSRFLIDTSILPLCLLNC

Note: Sequence incomplete. No experimental confirmation available.
Show »
Length:388
Mass (Da):44,487
Checksum:i126A576FFF4E5A86
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04086430E → Q in a breast infiltrating ductal carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_05165039E → A.Corresponds to variant rs7770402dbSNPEnsembl.1
Natural variantiVAR_04086550G → R.2 PublicationsCorresponds to variant rs2296356dbSNPEnsembl.1
Natural variantiVAR_04086678A → S in a breast infiltrating ductal carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant rs762093504dbSNPEnsembl.1
Natural variantiVAR_040867126T → M.1 PublicationCorresponds to variant rs34953021dbSNPEnsembl.1
Natural variantiVAR_040868217H → L in a lung squamous cell carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_040869318C → Y.1 PublicationCorresponds to variant rs35609073dbSNPEnsembl.1
Natural variantiVAR_040870373Q → R.1 PublicationCorresponds to variant rs35211631dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026948234 – 252LCVNR…FGLAR → QCSRFLIDTSILPLCLLNC in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138876, AL139092 Genomic DNA. Translation: CAI12221.2.
BC132831 mRNA. Translation: AAI32832.1.
BC132833 mRNA. Translation: AAI32834.1.
AK122581 mRNA. Translation: BAC56922.1.
CCDSiCCDS34330.1. [Q86YV6-1]
RefSeqiNP_001012418.2. NM_001012418.3. [Q86YV6-1]
XP_005249136.1. XM_005249079.2. [Q86YV6-1]
XP_016866308.1. XM_017010819.1. [Q86YV6-1]
UniGeneiHs.127830.

Genome annotation databases

EnsembliENST00000274643; ENSP00000274643; ENSG00000145949. [Q86YV6-1]
GeneIDi340156.
KEGGihsa:340156.
UCSCiuc003mty.5. human. [Q86YV6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL138876, AL139092 Genomic DNA. Translation: CAI12221.2.
BC132831 mRNA. Translation: AAI32832.1.
BC132833 mRNA. Translation: AAI32834.1.
AK122581 mRNA. Translation: BAC56922.1.
CCDSiCCDS34330.1. [Q86YV6-1]
RefSeqiNP_001012418.2. NM_001012418.3. [Q86YV6-1]
XP_005249136.1. XM_005249079.2. [Q86YV6-1]
XP_016866308.1. XM_017010819.1. [Q86YV6-1]
UniGeneiHs.127830.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2X4FX-ray2.67A/B40-388[»]
ProteinModelPortaliQ86YV6.
SMRiQ86YV6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131005. 8 interactors.
IntActiQ86YV6. 2 interactors.
STRINGi9606.ENSP00000274643.

Chemistry databases

BindingDBiQ86YV6.
ChEMBLiCHEMBL5426.
GuidetoPHARMACOLOGYi2111.

PTM databases

PhosphoSitePlusiQ86YV6.

Polymorphism and mutation databases

BioMutaiMYLK4.
DMDMi118573873.

Proteomic databases

MaxQBiQ86YV6.
PaxDbiQ86YV6.
PeptideAtlasiQ86YV6.
PRIDEiQ86YV6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274643; ENSP00000274643; ENSG00000145949. [Q86YV6-1]
GeneIDi340156.
KEGGihsa:340156.
UCSCiuc003mty.5. human. [Q86YV6-1]

Organism-specific databases

CTDi340156.
DisGeNETi340156.
GeneCardsiMYLK4.
H-InvDBHIX0018128.
HGNCiHGNC:27972. MYLK4.
HPAiHPA015860.
neXtProtiNX_Q86YV6.
OpenTargetsiENSG00000145949.
PharmGKBiPA162396406.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118877.
HOGENOMiHOG000233016.
HOVERGENiHBG095569.
InParanoidiQ86YV6.
KOiK00907.
OMAiFYTVSKT.
OrthoDBiEOG091G005W.
PhylomeDBiQ86YV6.
TreeFamiTF314166.

Enzyme and pathway databases

BioCyciZFISH:HS14121-MONOMER.
SignaLinkiQ86YV6.

Miscellaneous databases

EvolutionaryTraceiQ86YV6.
GenomeRNAii340156.
PROiQ86YV6.

Gene expression databases

BgeeiENSG00000145949.
CleanExiHS_MYLK4.
GenevisibleiQ86YV6. HS.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYLK4_HUMAN
AccessioniPrimary (citable) accession number: Q86YV6
Secondary accession number(s): A2RUC0, Q5TAW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.