Q86YV6 (MYLK4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain kinase family member 4
EC=2.7.11.1

Alternative name(s):
Sugen kinase 85
Short name=SgK085

Gene names

Name:	MYLK4
Synonyms:	SGK085

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86YV6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q86YV6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-252: LCVNRDAKQIKIIDFGLAR → QCSRFLIDTSILPLCLLNC

Note: Sequence incomplete. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 388

388

Myosin light chain kinase family member 4

PRO_0000261030

Regions

Domain

106 – 361

256

Protein kinase

Nucleotide binding

112 – 120

9

ATP By similarity

Sites

Active site

227

1

Proton acceptor By similarity

Binding site

135

1

ATP By similarity

Natural variations

Alternative sequence

234 – 252

19

LCVNR…FGLAR → QCSRFLIDTSILPLCLLNC in isoform 2.

VSP_026948

Natural variant

30

1

E → Q in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.5

VAR_040864

Natural variant

39

1

E → A.
Corresponds to variant rs7770402 [ dbSNP | Ensembl ].

VAR_051650

Natural variant

50

1

G → R. Ref.2 Ref.5
Corresponds to variant rs2296356 [ dbSNP | Ensembl ].

VAR_040865

Natural variant

78

1

A → S in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.5

VAR_040866

Natural variant

126

1

T → M. Ref.5
Corresponds to variant rs34953021 [ dbSNP | Ensembl ].

VAR_040867

Natural variant

217

1

H → L in a lung squamous cell carcinoma sample; somatic mutation. Ref.5

VAR_040868

Natural variant

318

1

C → Y. Ref.5
Corresponds to variant rs35609073 [ dbSNP | Ensembl ].

VAR_040869

Natural variant

373

1

Q → R. Ref.5
Corresponds to variant rs35211631 [ dbSNP | Ensembl ].

VAR_040870

Secondary structure

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388

Helix Strand Turn

Details...

Beta strand

96 – 98

3

Helix

100 – 102

3

Beta strand

104 – 111

8

Beta strand

120 – 125

6

Turn

126 – 128

3

Beta strand

131 – 138

8

Helix

142 – 155

14

Beta strand

166 – 171

6

Beta strand

173 – 181

9

Helix

189 – 194

6

Helix

196 – 198

3

Helix

201 – 220

20

Helix

230 – 232

3

Beta strand

233 – 237

5

Turn

238 – 241

4

Beta strand

242 – 245

4

Helix

272 – 275

4

Helix

282 – 298

17

Helix

308 – 317

10

Helix

325 – 327

3

Helix

332 – 339

8

Helix

346 – 348

3

Helix

352 – 357

6

Helix

359 – 362

4

Helix

364 – 372

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: EF710F6145DFC3C9	FASTA	388	44,508
	10 20 30 40 50 60 MLKVKRLEEF NTCYNSNQLE KMAFFQCREE VEKVKCFLEK NSGDQDSRSG HNEAKEVWSN 70 80 90 100 110 120 ADLTERMPVK SKRTSALAVD IPAPPAPFDH RIVTAKQGAV NSFYTVSKTE ILGGGRFGQV 130 140 150 160 170 180 HKCEETATGL KLAAKIIKTR GMKDKEEVKN EISVMNQLDH ANLIQLYDAF ESKNDIVLVM 190 200 210 220 230 240 EYVDGGELFD RIIDESYNLT ELDTILFMKQ ICEGIRHMHQ MYILHLDLKP ENILCVNRDA 250 260 270 280 290 300 KQIKIIDFGL ARRYKPREKL KVNFGTPEFL APEVVNYDFV SFPTDMWSVG VIAYMLLSGL 310 320 330 340 350 360 SPFLGDNDAE TLNNILACRW DLEDEEFQDI SEEAKEFISK LLIKEKSWRI SASEALKHPW 370 380 LSDHKLHSRL NAQKKKNRGS DAQDFVTK
	Isoform 2 [UniParc]. Checksum: 126A576FFF4E5A86	FASTA	388	44,487
	10 20 30 40 50 60 MLKVKRLEEF NTCYNSNQLE KMAFFQCREE VEKVKCFLEK NSGDQDSRSG HNEAKEVWSN 70 80 90 100 110 120 ADLTERMPVK SKRTSALAVD IPAPPAPFDH RIVTAKQGAV NSFYTVSKTE ILGGGRFGQV 130 140 150 160 170 180 HKCEETATGL KLAAKIIKTR GMKDKEEVKN EISVMNQLDH ANLIQLYDAF ESKNDIVLVM 190 200 210 220 230 240 EYVDGGELFD RIIDESYNLT ELDTILFMKQ ICEGIRHMHQ MYILHLDLKP ENIQCSRFLI 250 260 270 280 290 300 DTSILPLCLL NCRYKPREKL KVNFGTPEFL APEVVNYDFV SFPTDMWSVG VIAYMLLSGL 310 320 330 340 350 360 SPFLGDNDAE TLNNILACRW DLEDEEFQDI SEEAKEFISK LLIKEKSWRI SASEALKHPW 370 380 LSDHKLHSRL NAQKKKNRGS DAQDFVTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-50.
[3]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 234-388 (ISOFORM 2). Tissue: Spleen.
[4]	"The protein kinase complement of the human genome." Manning G., Whyte D.B., Martinez R., Hunter T., Sudarsanam S. Science 298:1912-1934(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLN-30; ARG-50; SER-78; MET-126; LEU-217; TYR-318 AND ARG-373.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL138876, AL139092 Genomic DNA. Translation: CAI12221.2.
BC132831 mRNA. Translation: AAI32832.1.
BC132833 mRNA. Translation: AAI32834.1.
AK122581 mRNA. Translation: BAC56922.1.

IPI

IPI01013835.
IPI01018630.

RefSeq

NP_001012418.2. NM_001012418.3.

UniGene

Hs.127830.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2X4F	X-ray	2.67	A/B	40-388	[»]

ProteinModelPortal

Q86YV6.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q86YV6. 1 interaction.

STRING

9606.ENSP00000274643.

PTM databases

PhosphoSite

Q86YV6.

Polymorphism databases

DMDM

118573873.

Proteomic databases

PaxDb

Q86YV6.

PRIDE

Q86YV6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000274643; ENSP00000274643; ENSG00000145949.

GeneID

340156.

KEGG

hsa:340156.

UCSC

uc003mty.4. human.

Organism-specific databases

CTD

340156.

GeneCards

GC06M002663.

H-InvDB

HIX0018128.

HGNC

HGNC:27972. MYLK4.

HPA

HPA015860.

neXtProt

NX_Q86YV6.

PharmGKB

PA162396406.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOGENOM

HOG000233016.

HOVERGEN

HBG095569.

InParanoid

Q86YV6.

KO

K00907.

OrthoDB

EOG4HMJ9T.

Gene expression databases

ArrayExpress

Q86YV6.

Bgee

Q86YV6.

CleanEx

HS_MYLK4.

Genevestigator

Q86YV6.

Family and domain databases

InterPro

IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

PANTHER

PTHR24347. PTHR24347. 1 hit.

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q86YV6.

ChEMBL

CHEMBL5426.

EvolutionaryTrace

Q86YV6.

GenomeRNAi

340156.

NextBio

97719.

Entry information

Entry name

MYLK4_HUMAN

Accession

Primary (citable) accession number: Q86YV6
Secondary accession number(s): A2RUC0, Q5TAW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.