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Q86YV6 (MYLK4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin light chain kinase family member 4

EC=2.7.11.1
Alternative name(s):
Sugen kinase 85
Short name=SgK085
Gene names
Name:MYLK4
Synonyms:SGK085
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86YV6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q86YV6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-252: LCVNRDAKQIKIIDFGLAR → QCSRFLIDTSILPLCLLNC
Note: Sequence incomplete. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 388388Myosin light chain kinase family member 4
PRO_0000261030

Regions

Domain106 – 361256Protein kinase
Nucleotide binding112 – 1209ATP By similarity

Sites

Active site2271Proton acceptor By similarity
Binding site1351ATP By similarity

Natural variations

Alternative sequence234 – 25219LCVNR…FGLAR → QCSRFLIDTSILPLCLLNC in isoform 2.
VSP_026948
Natural variant301E → Q in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.5
VAR_040864
Natural variant391E → A.
Corresponds to variant rs7770402 [ dbSNP | Ensembl ].
VAR_051650
Natural variant501G → R. Ref.2 Ref.5
Corresponds to variant rs2296356 [ dbSNP | Ensembl ].
VAR_040865
Natural variant781A → S in a breast infiltrating ductal carcinoma sample; somatic mutation. Ref.5
VAR_040866
Natural variant1261T → M. Ref.5
Corresponds to variant rs34953021 [ dbSNP | Ensembl ].
VAR_040867
Natural variant2171H → L in a lung squamous cell carcinoma sample; somatic mutation. Ref.5
VAR_040868
Natural variant3181C → Y. Ref.5
Corresponds to variant rs35609073 [ dbSNP | Ensembl ].
VAR_040869
Natural variant3731Q → R. Ref.5
Corresponds to variant rs35211631 [ dbSNP | Ensembl ].
VAR_040870

Secondary structure

............................................... 388
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: EF710F6145DFC3C9

FASTA38844,508
        10         20         30         40         50         60 
MLKVKRLEEF NTCYNSNQLE KMAFFQCREE VEKVKCFLEK NSGDQDSRSG HNEAKEVWSN 

        70         80         90        100        110        120 
ADLTERMPVK SKRTSALAVD IPAPPAPFDH RIVTAKQGAV NSFYTVSKTE ILGGGRFGQV 

       130        140        150        160        170        180 
HKCEETATGL KLAAKIIKTR GMKDKEEVKN EISVMNQLDH ANLIQLYDAF ESKNDIVLVM 

       190        200        210        220        230        240 
EYVDGGELFD RIIDESYNLT ELDTILFMKQ ICEGIRHMHQ MYILHLDLKP ENILCVNRDA 

       250        260        270        280        290        300 
KQIKIIDFGL ARRYKPREKL KVNFGTPEFL APEVVNYDFV SFPTDMWSVG VIAYMLLSGL 

       310        320        330        340        350        360 
SPFLGDNDAE TLNNILACRW DLEDEEFQDI SEEAKEFISK LLIKEKSWRI SASEALKHPW 

       370        380 
LSDHKLHSRL NAQKKKNRGS DAQDFVTK 

« Hide

Isoform 2 [UniParc].

Checksum: 126A576FFF4E5A86
Show »

FASTA38844,487

References

« Hide 'large scale' references
[1]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-50.
[3]"The nucleotide sequence of a long cDNA clone isolated from human spleen."
Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 234-388 (ISOFORM 2).
Tissue: Spleen.
[4]"The protein kinase complement of the human genome."
Manning G., Whyte D.B., Martinez R., Hunter T., Sudarsanam S.
Science 298:1912-1934(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLN-30; ARG-50; SER-78; MET-126; LEU-217; TYR-318 AND ARG-373.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL138876, AL139092 Genomic DNA. Translation: CAI12221.2.
BC132831 mRNA. Translation: AAI32832.1.
BC132833 mRNA. Translation: AAI32834.1.
AK122581 mRNA. Translation: BAC56922.1.
RefSeqNP_001012418.2. NM_001012418.3.
XP_005249134.1. XM_005249077.1.
XP_005249135.1. XM_005249078.1.
XP_005249136.1. XM_005249079.1.
UniGeneHs.127830.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2X4FX-ray2.67A/B40-388[»]
ProteinModelPortalQ86YV6.
SMRQ86YV6. Positions 81-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid131005. 2 interactions.
IntActQ86YV6. 1 interaction.
STRING9606.ENSP00000274643.

Chemistry

BindingDBQ86YV6.
ChEMBLCHEMBL5426.
GuidetoPHARMACOLOGY2111.

PTM databases

PhosphoSiteQ86YV6.

Polymorphism databases

DMDM118573873.

Proteomic databases

PaxDbQ86YV6.
PRIDEQ86YV6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274643; ENSP00000274643; ENSG00000145949. [Q86YV6-1]
GeneID340156.
KEGGhsa:340156.
UCSCuc003mty.4. human. [Q86YV6-1]

Organism-specific databases

CTD340156.
GeneCardsGC06M002663.
H-InvDBHIX0018128.
HGNCHGNC:27972. MYLK4.
HPAHPA015860.
neXtProtNX_Q86YV6.
PharmGKBPA162396406.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233016.
HOVERGENHBG095569.
InParanoidQ86YV6.
KOK00907.
OrthoDBEOG73FQMV.
PhylomeDBQ86YV6.
TreeFamTF314166.

Enzyme and pathway databases

SignaLinkQ86YV6.

Gene expression databases

ArrayExpressQ86YV6.
BgeeQ86YV6.
CleanExHS_MYLK4.
GenevestigatorQ86YV6.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ86YV6.
GenomeRNAi340156.
NextBio97719.
PROQ86YV6.

Entry information

Entry nameMYLK4_HUMAN
AccessionPrimary (citable) accession number: Q86YV6
Secondary accession number(s): A2RUC0, Q5TAW2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM