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Q86YV6

- MYLK4_HUMAN

UniProt

Q86YV6 - MYLK4_HUMAN

Protein

Myosin light chain kinase family member 4

Gene

MYLK4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei135 – 1351ATPPROSITE-ProRule annotation
    Active sitei227 – 2271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi112 – 1209ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ86YV6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain kinase family member 4 (EC:2.7.11.1)
    Alternative name(s):
    Sugen kinase 85
    Short name:
    SgK085
    Gene namesi
    Name:MYLK4
    Synonyms:SGK085
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:27972. MYLK4.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162396406.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Myosin light chain kinase family member 4PRO_0000261030Add
    BLAST

    Proteomic databases

    PaxDbiQ86YV6.
    PRIDEiQ86YV6.

    PTM databases

    PhosphoSiteiQ86YV6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ86YV6.
    BgeeiQ86YV6.
    CleanExiHS_MYLK4.
    GenevestigatoriQ86YV6.

    Organism-specific databases

    HPAiHPA015860.

    Interactioni

    Protein-protein interaction databases

    BioGridi131005. 2 interactions.
    IntActiQ86YV6. 2 interactions.
    STRINGi9606.ENSP00000274643.

    Structurei

    Secondary structure

    1
    388
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi96 – 983
    Helixi100 – 1023
    Beta strandi104 – 1118
    Beta strandi120 – 1256
    Turni126 – 1283
    Beta strandi131 – 1388
    Helixi142 – 15514
    Beta strandi166 – 1716
    Beta strandi173 – 1819
    Helixi189 – 1946
    Helixi196 – 1983
    Helixi201 – 22020
    Helixi230 – 2323
    Beta strandi233 – 2375
    Turni238 – 2414
    Beta strandi242 – 2454
    Helixi272 – 2754
    Helixi282 – 29817
    Helixi308 – 31710
    Helixi325 – 3273
    Helixi332 – 3398
    Helixi346 – 3483
    Helixi352 – 3576
    Helixi359 – 3624
    Helixi364 – 3729

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2X4FX-ray2.67A/B40-388[»]
    ProteinModelPortaliQ86YV6.
    SMRiQ86YV6. Positions 81-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ86YV6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini106 – 361256Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233016.
    HOVERGENiHBG095569.
    InParanoidiQ86YV6.
    KOiK00907.
    OrthoDBiEOG73FQMV.
    PhylomeDBiQ86YV6.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86YV6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLKVKRLEEF NTCYNSNQLE KMAFFQCREE VEKVKCFLEK NSGDQDSRSG    50
    HNEAKEVWSN ADLTERMPVK SKRTSALAVD IPAPPAPFDH RIVTAKQGAV 100
    NSFYTVSKTE ILGGGRFGQV HKCEETATGL KLAAKIIKTR GMKDKEEVKN 150
    EISVMNQLDH ANLIQLYDAF ESKNDIVLVM EYVDGGELFD RIIDESYNLT 200
    ELDTILFMKQ ICEGIRHMHQ MYILHLDLKP ENILCVNRDA KQIKIIDFGL 250
    ARRYKPREKL KVNFGTPEFL APEVVNYDFV SFPTDMWSVG VIAYMLLSGL 300
    SPFLGDNDAE TLNNILACRW DLEDEEFQDI SEEAKEFISK LLIKEKSWRI 350
    SASEALKHPW LSDHKLHSRL NAQKKKNRGS DAQDFVTK 388

    Note: No experimental confirmation available.

    Length:388
    Mass (Da):44,508
    Last modified:November 28, 2006 - v2
    Checksum:iEF710F6145DFC3C9
    GO
    Isoform 2 (identifier: Q86YV6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         234-252: LCVNRDAKQIKIIDFGLAR → QCSRFLIDTSILPLCLLNC

    Note: Sequence incomplete. No experimental confirmation available.

    Show »
    Length:388
    Mass (Da):44,487
    Checksum:i126A576FFF4E5A86
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti30 – 301E → Q in a breast infiltrating ductal carcinoma sample; somatic mutation. 1 Publication
    VAR_040864
    Natural varianti39 – 391E → A.
    Corresponds to variant rs7770402 [ dbSNP | Ensembl ].
    VAR_051650
    Natural varianti50 – 501G → R.2 Publications
    Corresponds to variant rs2296356 [ dbSNP | Ensembl ].
    VAR_040865
    Natural varianti78 – 781A → S in a breast infiltrating ductal carcinoma sample; somatic mutation. 1 Publication
    VAR_040866
    Natural varianti126 – 1261T → M.1 Publication
    Corresponds to variant rs34953021 [ dbSNP | Ensembl ].
    VAR_040867
    Natural varianti217 – 2171H → L in a lung squamous cell carcinoma sample; somatic mutation. 1 Publication
    VAR_040868
    Natural varianti318 – 3181C → Y.1 Publication
    Corresponds to variant rs35609073 [ dbSNP | Ensembl ].
    VAR_040869
    Natural varianti373 – 3731Q → R.1 Publication
    Corresponds to variant rs35211631 [ dbSNP | Ensembl ].
    VAR_040870

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei234 – 25219LCVNR…FGLAR → QCSRFLIDTSILPLCLLNC in isoform 2. 1 PublicationVSP_026948Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138876, AL139092 Genomic DNA. Translation: CAI12221.2.
    BC132831 mRNA. Translation: AAI32832.1.
    BC132833 mRNA. Translation: AAI32834.1.
    AK122581 mRNA. Translation: BAC56922.1.
    CCDSiCCDS34330.1. [Q86YV6-1]
    RefSeqiNP_001012418.2. NM_001012418.3. [Q86YV6-1]
    XP_005249134.1. XM_005249077.1. [Q86YV6-1]
    XP_005249135.1. XM_005249078.1. [Q86YV6-1]
    XP_005249136.1. XM_005249079.1. [Q86YV6-1]
    UniGeneiHs.127830.

    Genome annotation databases

    EnsembliENST00000274643; ENSP00000274643; ENSG00000145949. [Q86YV6-1]
    GeneIDi340156.
    KEGGihsa:340156.
    UCSCiuc003mty.4. human. [Q86YV6-1]

    Polymorphism databases

    DMDMi118573873.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL138876 , AL139092 Genomic DNA. Translation: CAI12221.2 .
    BC132831 mRNA. Translation: AAI32832.1 .
    BC132833 mRNA. Translation: AAI32834.1 .
    AK122581 mRNA. Translation: BAC56922.1 .
    CCDSi CCDS34330.1. [Q86YV6-1 ]
    RefSeqi NP_001012418.2. NM_001012418.3. [Q86YV6-1 ]
    XP_005249134.1. XM_005249077.1. [Q86YV6-1 ]
    XP_005249135.1. XM_005249078.1. [Q86YV6-1 ]
    XP_005249136.1. XM_005249079.1. [Q86YV6-1 ]
    UniGenei Hs.127830.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2X4F X-ray 2.67 A/B 40-388 [» ]
    ProteinModelPortali Q86YV6.
    SMRi Q86YV6. Positions 81-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 131005. 2 interactions.
    IntActi Q86YV6. 2 interactions.
    STRINGi 9606.ENSP00000274643.

    Chemistry

    BindingDBi Q86YV6.
    ChEMBLi CHEMBL5426.
    GuidetoPHARMACOLOGYi 2111.

    PTM databases

    PhosphoSitei Q86YV6.

    Polymorphism databases

    DMDMi 118573873.

    Proteomic databases

    PaxDbi Q86YV6.
    PRIDEi Q86YV6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000274643 ; ENSP00000274643 ; ENSG00000145949 . [Q86YV6-1 ]
    GeneIDi 340156.
    KEGGi hsa:340156.
    UCSCi uc003mty.4. human. [Q86YV6-1 ]

    Organism-specific databases

    CTDi 340156.
    GeneCardsi GC06M002663.
    H-InvDB HIX0018128.
    HGNCi HGNC:27972. MYLK4.
    HPAi HPA015860.
    neXtProti NX_Q86YV6.
    PharmGKBi PA162396406.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233016.
    HOVERGENi HBG095569.
    InParanoidi Q86YV6.
    KOi K00907.
    OrthoDBi EOG73FQMV.
    PhylomeDBi Q86YV6.
    TreeFami TF314166.

    Enzyme and pathway databases

    SignaLinki Q86YV6.

    Miscellaneous databases

    EvolutionaryTracei Q86YV6.
    GenomeRNAii 340156.
    NextBioi 97719.
    PROi Q86YV6.

    Gene expression databases

    ArrayExpressi Q86YV6.
    Bgeei Q86YV6.
    CleanExi HS_MYLK4.
    Genevestigatori Q86YV6.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-50.
    3. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
      Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 234-388 (ISOFORM 2).
      Tissue: Spleen.
    4. Cited for: IDENTIFICATION.
    5. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLN-30; ARG-50; SER-78; MET-126; LEU-217; TYR-318 AND ARG-373.

    Entry informationi

    Entry nameiMYLK4_HUMAN
    AccessioniPrimary (citable) accession number: Q86YV6
    Secondary accession number(s): A2RUC0, Q5TAW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3