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Protein

RAS protein activator like-3

Gene

RASAL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a Ras GTPase-activating protein. Plays an important role in the expansion and functions of natural killer T (NKT) cells in the liver by negatively regulating RAS activity and the down-stream ERK signaling pathway.By similarity

GO - Molecular functioni

GO - Biological processi

  • MAPK cascade Source: Reactome
  • negative regulation of Ras protein signal transduction Source: UniProtKB
  • positive regulation of NK T cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Names & Taxonomyi

Protein namesi
Recommended name:
RAS protein activator like-3
Gene namesi
Name:RASAL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:26129. RASAL3.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB
  • cytoplasmic side of membrane Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • intrinsic component of the cytoplasmic side of the plasma membrane Source: GO_Central
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000105122.
PharmGKBiPA164725297.

Polymorphism and mutation databases

BioMutaiRASAL3.
DMDMi172046741.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003225661 – 1011RAS protein activator like-3Add BLAST1011

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineCombined sources1
Modified residuei51PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei166PhosphoserineBy similarity1
Modified residuei167PhosphoserineCombined sources1
Modified residuei170PhosphoserineBy similarity1
Modified residuei224PhosphoserineBy similarity1
Modified residuei228PhosphoserineBy similarity1
Modified residuei231PhosphoserineCombined sources1
Modified residuei234PhosphothreonineBy similarity1
Modified residuei787PhosphoserineBy similarity1
Modified residuei790PhosphoserineBy similarity1
Modified residuei988PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86YV0.
MaxQBiQ86YV0.
PaxDbiQ86YV0.
PeptideAtlasiQ86YV0.
PRIDEiQ86YV0.

PTM databases

iPTMnetiQ86YV0.
PhosphoSitePlusiQ86YV0.

Expressioni

Tissue specificityi

Predominantly expressed in cells of hematopoietic lineages.1 Publication

Gene expression databases

BgeeiENSG00000105122.
ExpressionAtlasiQ86YV0. baseline and differential.
GenevisibleiQ86YV0. HS.

Organism-specific databases

HPAiHPA043417.

Interactioni

Protein-protein interaction databases

BioGridi122348. 2 interactors.
IntActiQ86YV0. 22 interactors.
STRINGi9606.ENSP00000341905.

Structurei

3D structure databases

ProteinModelPortaliQ86YV0.
SMRiQ86YV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 293PHAdd BLAST97
Domaini306 – 384C2Add BLAST79
Domaini458 – 650Ras-GAPPROSITE-ProRule annotationAdd BLAST193

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili888 – 988Sequence analysisAdd BLAST101

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi28 – 31Poly-Gly4
Compositional biasi96 – 133Pro-richAdd BLAST38
Compositional biasi789 – 829Arg-richAdd BLAST41

Sequence similaritiesi

Contains 1 C2 domain.Curated
Contains 1 PH domain.Curated
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3508. Eukaryota.
ENOG410XPU1. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000154074.
InParanoidiQ86YV0.
KOiK17634.
OMAiRRHFQPS.
OrthoDBiEOG091G020W.
PhylomeDBiQ86YV0.
TreeFamiTF105303.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00616. RasGAP. 2 hits.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YV0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPPSPSRTS QTQPTATSPL TSYRWHTGGG GEKAAGGFRW GRFAGWGRAL
60 70 80 90 100
SHQEPMVSTQ PAPRSIFRRV LSAPPKESRT SRLRLSKALW GRHKNPPPEP
110 120 130 140 150
DPEPEQEAPE LEPEPELEPP TPQIPEAPTP NVPVWDIGGF TLLDGKLVLL
160 170 180 190 200
GGEEEGPRRP RVGSASSEGS IHVAMGNFRD PDRMPGKTEP ETAGPNQVHN
210 220 230 240 250
VRGLLKRLKE KKKARLEPRD GPPSALGSRE SLATLSELDL GAERDVRIWP
260 270 280 290 300
LHPSLLGEPH CFQVTWTGGS RCFSCRSAAE RDRWIEDLRR QFQPTQDNVE
310 320 330 340 350
REETWLSVWV HEAKGLPRAA AGAPGVRAEL WLDGALLART APRAGPGQLF
360 370 380 390 400
WAERFHFEAL PPARRLSLRL RGLGPGSAVL GRVALALEEL DAPRAPAAGL
410 420 430 440 450
ERWFPLLGAP AGAALRARIR ARRLRVLPSE RYKELAEFLT FHYARLCGAL
460 470 480 490 500
EPALPAQAKE ELAAAMVRVL RATGRAQALV TDLGTAELAR CGGREALLFR
510 520 530 540 550
ENTLATKAID EYMKLVAQDY LQETLGQVVR RLCASTEDCE VDPSKCPASE
560 570 580 590 600
LPEHQARLRN SCEEVFETII HSYDWFPAEL GIVFSSWREA CKERGSEVLG
610 620 630 640 650
PRLVCASLFL RLLCPAILAP SLFGLAPDHP APGPARTLTL IAKVIQNLAN
660 670 680 690 700
RAPFGEKEAY MGFMNSFLEE HGPAMQCFLD QVAMVDVDAA PSGYQGSGDL
710 720 730 740 750
ALQLAVLHAQ LCTIFAELDQ TTRDTLEPLP TILRAIEEGQ PVLVSVPMRL
760 770 780 790 800
PLPPAQVHSS LSAGEKPGFL APRDLPKHTP LISKSQSLRS VRRSESWARP
810 820 830 840 850
RPDEERPLRR PRPVQRTQSV PVRRPARRRQ SAGPWPRPKG SLSMGPAPRA
860 870 880 890 900
RPWTRDSASL PRKPSVPWQR QMDQPQDRNQ ALGTHRPVNK LAELQCEVAA
910 920 930 940 950
LREEQKVLSR LVESLSTQIR ALTEQQEQLR GQLQDLDSRL RAGSSEFDSE
960 970 980 990 1000
HNLTSNEGHS LKNLEHRLNE MERTQAQLRD AVQSLQLSPR TRGSWSQPQP
1010
LKAPCLNGDT T
Length:1,011
Mass (Da):111,898
Last modified:February 26, 2008 - v2
Checksum:i23A7CA251ED3CE33
GO
Isoform 2 (identifier: Q86YV0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     944-1011: Missing.

Show »
Length:943
Mass (Da):104,243
Checksum:i2DD6F0CCA9FCA2A0
GO

Sequence cautioni

The sequence AAH30281 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB15064 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC56928 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC56928 differs from that shown. Reason: Erroneous termination at position 723. Translated as Arg.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061179251L → V.Corresponds to variant rs58123634dbSNPEnsembl.1
Natural variantiVAR_061180594R → C.Corresponds to variant rs56209154dbSNPEnsembl.1
Natural variantiVAR_061181825P → T.Corresponds to variant rs57208996dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_033824944 – 1011Missing in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122587 mRNA. Translation: BAC56928.1. Sequence problems.
AC011492 Genomic DNA. No translation available.
AK025091 mRNA. Translation: BAB15064.1. Different initiation.
BC030281 mRNA. Translation: AAH30281.1. Different initiation.
CCDSiCCDS46006.1. [Q86YV0-1]
RefSeqiNP_075055.1. NM_022904.1. [Q86YV0-1]
UniGeneiHs.136979.

Genome annotation databases

EnsembliENST00000343625; ENSP00000341905; ENSG00000105122. [Q86YV0-1]
GeneIDi64926.
KEGGihsa:64926.
UCSCiuc002nbe.3. human. [Q86YV0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122587 mRNA. Translation: BAC56928.1. Sequence problems.
AC011492 Genomic DNA. No translation available.
AK025091 mRNA. Translation: BAB15064.1. Different initiation.
BC030281 mRNA. Translation: AAH30281.1. Different initiation.
CCDSiCCDS46006.1. [Q86YV0-1]
RefSeqiNP_075055.1. NM_022904.1. [Q86YV0-1]
UniGeneiHs.136979.

3D structure databases

ProteinModelPortaliQ86YV0.
SMRiQ86YV0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122348. 2 interactors.
IntActiQ86YV0. 22 interactors.
STRINGi9606.ENSP00000341905.

PTM databases

iPTMnetiQ86YV0.
PhosphoSitePlusiQ86YV0.

Polymorphism and mutation databases

BioMutaiRASAL3.
DMDMi172046741.

Proteomic databases

EPDiQ86YV0.
MaxQBiQ86YV0.
PaxDbiQ86YV0.
PeptideAtlasiQ86YV0.
PRIDEiQ86YV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343625; ENSP00000341905; ENSG00000105122. [Q86YV0-1]
GeneIDi64926.
KEGGihsa:64926.
UCSCiuc002nbe.3. human. [Q86YV0-1]

Organism-specific databases

CTDi64926.
GeneCardsiRASAL3.
H-InvDBHIX0202731.
HGNCiHGNC:26129. RASAL3.
HPAiHPA043417.
MIMi616561. gene.
neXtProtiNX_Q86YV0.
OpenTargetsiENSG00000105122.
PharmGKBiPA164725297.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3508. Eukaryota.
ENOG410XPU1. LUCA.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000154074.
InParanoidiQ86YV0.
KOiK17634.
OMAiRRHFQPS.
OrthoDBiEOG091G020W.
PhylomeDBiQ86YV0.
TreeFamiTF105303.

Enzyme and pathway databases

ReactomeiR-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-8849471. PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.

Miscellaneous databases

ChiTaRSiRASAL3. human.
GenomeRNAii64926.
PROiQ86YV0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105122.
ExpressionAtlasiQ86YV0. baseline and differential.
GenevisibleiQ86YV0. HS.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR011993. PH_dom-like.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00616. RasGAP. 2 hits.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRASL3_HUMAN
AccessioniPrimary (citable) accession number: Q86YV0
Secondary accession number(s): Q8N2T9, Q9H735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.