Q86YV0 (RASL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RAS protein activator like-3

Gene names

Name:

RASAL3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1011 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a Ras GTPase-activating protein By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain.
Sequence caution	The sequence AAH30281.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15064.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC56928.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAC56928.1 differs from that shown. Reason: Erroneous termination at position 723. Translated as Arg.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of Ras protein signal transduction Inferred from Biological aspect of Ancestor. Source: RefGenome signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome intrinsic to internal side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	Ras GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86YV0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86YV0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     944-1011: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1011	1011	RAS protein activator like-3		PRO_0000322566
Regions
	Domain	197 – 293	97	PH
	Domain	306 – 384	79	C2
	Domain	458 – 650	193	Ras-GAP
	Coiled coil	888 – 988	101	Potential
	Compositional bias	28 – 31	4	Poly-Gly
	Compositional bias	96 – 133	38	Pro-rich
	Compositional bias	789 – 829	41	Arg-rich
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.5
	Modified residue	164	1	Phosphoserine Ref.5
	Modified residue	167	1	Phosphoserine Ref.5
	Modified residue	231	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	944 – 1011	68	Missing in isoform 2.		VSP_033824
	Natural variant	251	1	L → V. Corresponds to variant rs58123634 [ dbSNP | Ensembl ].		VAR_061179
	Natural variant	594	1	R → C. Corresponds to variant rs56209154 [ dbSNP | Ensembl ].		VAR_061180
	Natural variant	825	1	P → T. Corresponds to variant rs57208996 [ dbSNP | Ensembl ].		VAR_061181

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 23A7CA251ED3CE33	FASTA	1,011	111,898
	10 20 30 40 50 60 MDPPSPSRTS QTQPTATSPL TSYRWHTGGG GEKAAGGFRW GRFAGWGRAL SHQEPMVSTQ 70 80 90 100 110 120 PAPRSIFRRV LSAPPKESRT SRLRLSKALW GRHKNPPPEP DPEPEQEAPE LEPEPELEPP 130 140 150 160 170 180 TPQIPEAPTP NVPVWDIGGF TLLDGKLVLL GGEEEGPRRP RVGSASSEGS IHVAMGNFRD 190 200 210 220 230 240 PDRMPGKTEP ETAGPNQVHN VRGLLKRLKE KKKARLEPRD GPPSALGSRE SLATLSELDL 250 260 270 280 290 300 GAERDVRIWP LHPSLLGEPH CFQVTWTGGS RCFSCRSAAE RDRWIEDLRR QFQPTQDNVE 310 320 330 340 350 360 REETWLSVWV HEAKGLPRAA AGAPGVRAEL WLDGALLART APRAGPGQLF WAERFHFEAL 370 380 390 400 410 420 PPARRLSLRL RGLGPGSAVL GRVALALEEL DAPRAPAAGL ERWFPLLGAP AGAALRARIR 430 440 450 460 470 480 ARRLRVLPSE RYKELAEFLT FHYARLCGAL EPALPAQAKE ELAAAMVRVL RATGRAQALV 490 500 510 520 530 540 TDLGTAELAR CGGREALLFR ENTLATKAID EYMKLVAQDY LQETLGQVVR RLCASTEDCE 550 560 570 580 590 600 VDPSKCPASE LPEHQARLRN SCEEVFETII HSYDWFPAEL GIVFSSWREA CKERGSEVLG 610 620 630 640 650 660 PRLVCASLFL RLLCPAILAP SLFGLAPDHP APGPARTLTL IAKVIQNLAN RAPFGEKEAY 670 680 690 700 710 720 MGFMNSFLEE HGPAMQCFLD QVAMVDVDAA PSGYQGSGDL ALQLAVLHAQ LCTIFAELDQ 730 740 750 760 770 780 TTRDTLEPLP TILRAIEEGQ PVLVSVPMRL PLPPAQVHSS LSAGEKPGFL APRDLPKHTP 790 800 810 820 830 840 LISKSQSLRS VRRSESWARP RPDEERPLRR PRPVQRTQSV PVRRPARRRQ SAGPWPRPKG 850 860 870 880 890 900 SLSMGPAPRA RPWTRDSASL PRKPSVPWQR QMDQPQDRNQ ALGTHRPVNK LAELQCEVAA 910 920 930 940 950 960 LREEQKVLSR LVESLSTQIR ALTEQQEQLR GQLQDLDSRL RAGSSEFDSE HNLTSNEGHS 970 980 990 1000 1010 LKNLEHRLNE MERTQAQLRD AVQSLQLSPR TRGSWSQPQP LKAPCLNGDT T
	Isoform 2 [UniParc]. Checksum: 2DD6F0CCA9FCA2A0	FASTA	943	104,243
	10 20 30 40 50 60 MDPPSPSRTS QTQPTATSPL TSYRWHTGGG GEKAAGGFRW GRFAGWGRAL SHQEPMVSTQ 70 80 90 100 110 120 PAPRSIFRRV LSAPPKESRT SRLRLSKALW GRHKNPPPEP DPEPEQEAPE LEPEPELEPP 130 140 150 160 170 180 TPQIPEAPTP NVPVWDIGGF TLLDGKLVLL GGEEEGPRRP RVGSASSEGS IHVAMGNFRD 190 200 210 220 230 240 PDRMPGKTEP ETAGPNQVHN VRGLLKRLKE KKKARLEPRD GPPSALGSRE SLATLSELDL 250 260 270 280 290 300 GAERDVRIWP LHPSLLGEPH CFQVTWTGGS RCFSCRSAAE RDRWIEDLRR QFQPTQDNVE 310 320 330 340 350 360 REETWLSVWV HEAKGLPRAA AGAPGVRAEL WLDGALLART APRAGPGQLF WAERFHFEAL 370 380 390 400 410 420 PPARRLSLRL RGLGPGSAVL GRVALALEEL DAPRAPAAGL ERWFPLLGAP AGAALRARIR 430 440 450 460 470 480 ARRLRVLPSE RYKELAEFLT FHYARLCGAL EPALPAQAKE ELAAAMVRVL RATGRAQALV 490 500 510 520 530 540 TDLGTAELAR CGGREALLFR ENTLATKAID EYMKLVAQDY LQETLGQVVR RLCASTEDCE 550 560 570 580 590 600 VDPSKCPASE LPEHQARLRN SCEEVFETII HSYDWFPAEL GIVFSSWREA CKERGSEVLG 610 620 630 640 650 660 PRLVCASLFL RLLCPAILAP SLFGLAPDHP APGPARTLTL IAKVIQNLAN RAPFGEKEAY 670 680 690 700 710 720 MGFMNSFLEE HGPAMQCFLD QVAMVDVDAA PSGYQGSGDL ALQLAVLHAQ LCTIFAELDQ 730 740 750 760 770 780 TTRDTLEPLP TILRAIEEGQ PVLVSVPMRL PLPPAQVHSS LSAGEKPGFL APRDLPKHTP 790 800 810 820 830 840 LISKSQSLRS VRRSESWARP RPDEERPLRR PRPVQRTQSV PVRRPARRRQ SAGPWPRPKG 850 860 870 880 890 900 SLSMGPAPRA RPWTRDSASL PRKPSVPWQR QMDQPQDRNQ ALGTHRPVNK LAELQCEVAA 910 920 930 940 LREEQKVLSR LVESLSTQIR ALTEQQEQLR GQLQDLDSRL RAG

References

[1]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 715-1011 (ISOFORM 2). Tissue: Colon.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 723-1011 (ISOFORM 1). Tissue: Blood.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-164; SER-167 AND SER-231, MASS SPECTROMETRY. Tissue: Leukemic T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK122587 mRNA. Translation: BAC56928.1. Sequence problems. AC011492 Genomic DNA. No translation available. AK025091 mRNA. Translation: BAB15064.1. Different initiation. BC030281 mRNA. Translation: AAH30281.1. Different initiation.
IPI	IPI00329668. IPI00893992.
RefSeq	NP_075055.1. NM_022904.1.
UniGene	Hs.136979.
3D structure databases
HSSP	HSSP built from PDB template 1WQ1 based on UniProtKB P20936.
ProteinModelPortal	Q86YV0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86YV0. 2 interactions.
STRING	9606.ENSP00000341905.
PTM databases
PhosphoSite	Q86YV0.
Polymorphism databases
DMDM	172046741.
Proteomic databases
PaxDb	Q86YV0.
PRIDE	Q86YV0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000343625; ENSP00000341905; ENSG00000105122.
GeneID	64926.
KEGG	hsa:64926.
UCSC	uc002nbe.2. human.
Organism-specific databases
CTD	64926.
GeneCards	GC19M015562.
H-InvDB	HIX0202731.
HGNC	HGNC:26129. RASAL3.
HPA	HPA043417.
neXtProt	NX_Q86YV0.
PharmGKB	PA164725297.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG313834.
HOGENOM	HOG000154074.
InParanoid	Q86YV0.
OMA	LPSERYK.
OrthoDB	EOG4SF958.
PhylomeDB	Q86YV0.
Gene expression databases
Bgee	Q86YV0.
Genevestigator	Q86YV0.
Family and domain databases
Gene3D	1.10.506.10. 1 hit.
InterPro	IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR001849. Pleckstrin_homology. IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. [Graphical view]
Pfam	PF00616. RasGAP. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00323. RasGAP. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50004. C2. False negative. PS50003. PH_DOMAIN. False negative. PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64926.
NextBio	67081.

Entry information

Entry name

RASL3_HUMAN

Accession

Primary (citable) accession number: Q86YV0
Secondary accession number(s): Q8N2T9, Q9H735

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.