E3 ubiquitin-protein ligase MIB1 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    E3 ubiquitin-protein ligase MIB1
    EC=6.3.2.-
Alternative name(s):
    Mind bomb homolog 1
    DAPK-interacting protein 1
      Short name=DIP-1
    Zinc finger ZZ type with ankyrin repeat domain protein 2

Gene names

Name:	MIB1
Synonyms:	DIP1, KIAA1323, ZZANK2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length	1006 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

Hide | Top

General annotation (Comments)

Function	E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis By similarity.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Cytoplasm. Cell membrane By similarity. Note: Localizes to the plasma membrane By similarity. According to Ref.8, it is mitochondrial, however such localization remains unclear.
Tissue specificity	Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined. Ref.6
Post-translational modification	Ubiquitinated. Possibly via autoubiquitination By similarity.
Miscellaneous	In epilepsy brain tissue, levels of expression are increased in the cytoplasm and microsomal fractions (endoplasmic reticulum).
Sequence similarities	Contains 9 ANK repeats. Contains 2 MIB/HERC2 domains. Contains 3 RING-type zinc fingers. Contains 1 ZZ-type zinc finger.
Sequence caution	The sequence AAN18023.1 differs from that shown. Reason: Frameshift at position 3.

Hide | Top

Ontologies

Keywords
Biological process	Notch signaling pathway Ubl conjugation pathway
Cellular component	Cell membrane Cytoplasm Membrane
Domain	ANK repeat Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Ubl conjugation
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1006

1006

E3 ubiquitin-protein ligase MIB1

PRO_0000055943

Regions

Domain

6 – 74

69

MIB/HERC2 1

Domain

143 – 221

79

MIB/HERC2 2

Repeat

430 – 460

31

ANK 1

Repeat

463 – 492

30

ANK 2

Repeat

496 – 525

30

ANK 3

Repeat

529 – 558

30

ANK 4

Repeat

562 – 591

30

ANK 5

Repeat

595 – 627

33

ANK 6

Repeat

631 – 661

31

ANK 7

Repeat

665 – 694

30

ANK 8

Repeat

698 – 729

32

ANK 9

Zinc finger

79 – 126

48

ZZ-type

Zinc finger

819 – 854

36

RING-type 1

Zinc finger

866 – 901

36

RING-type 2

Zinc finger

963 – 996

34

RING-type 3

Coiled coil

935 – 962

28

Potential

Experimental info

Sequence conflict

327

1

E → K in AAN18023. Ref.2

Sequence conflict

398

1

S → F in AAN18023. Ref.2

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q86YT6-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5D7D0D91AF98FF18
Show »

FASTA

1,006

110,136

        10         20         30         40         50         60 
MSNSRNNRVM VEGVGARVVR GPDWKWGKQD GGEGHVGTVR SFESPEEVVV VWDNGTAANY 

        70         80         90        100        110        120 
RCSGAYDLRI LDSAPTGIKH DGTMCDTCRQ QPIIGIRWKC AECTNYDLCT VCYHGDKHHL 

       130        140        150        160        170        180 
RHRFYRITTP GSERVLLESR RKSKKITARG IFAGARVVRG VDWQWEDQDG GNGRRGKVTE 

       190        200        210        220        230        240 
IQDWSASSPH SAAYVLWDNG AKNLYRVGFE GMSDLKCVQD AKGGSFYRDH CPVLGEQNGN 

       250        260        270        280        290        300 
RNPGGLQIGD LVNIDLDLEI VQSLQHGHGG WTDGMFETLT TTGTVCGIDE DHDIVVQYPS 

       310        320        330        340        350        360 
GNRWTFNPAV LTKANIVRSG DAAQGAEGGT SQFQVGDLVQ VCYDLERIKL LQRGHGEWAE 

       370        380        390        400        410        420 
AMLPTLGKVG RVQQIYSDSD LKVEVCGTSW TYNPAAVSKV ASAGSAISNA SGERLSQLLK 

       430        440        450        460        470        480 
KLFETQESGD LNEELVKAAA NGDVAKVEDL LKRPDVDVNG QCAGHTAMQA ASQNGHVDIL 

       490        500        510        520        530        540 
KLLLKQNVDV EAEDKDGDRA VHHAAFGDEG AVIEVLHRGS ADLNARNKRR QTPLHIAVNK 

       550        560        570        580        590        600 
GHLQVVKTLL DFGCHPSLQD SEGDTPLHDA ISKKRDDILA VLLEAGADVT ITNNNGFNAL 

       610        620        630        640        650        660 
HHAALRGNPS AMRVLLSKLP RPWIVDEKKD DGYTALHLAA LNNHVEVAEL LVHQGNANLD 

       670        680        690        700        710        720 
IQNVNQQTAL HLAVERQHTQ IVRLLVRAGA KLDIQDKDGD TPLHEALRHH TLSQLRQLQD 

       730        740        750        760        770        780 
MQDVGKVDAA WEPSKNTLIM GLGTQGAEKK SAASIACFLA ANGADLSIRN KKGQSPLDLC 

       790        800        810        820        830        840 
PDPNLCKALA KCHKEKVSGQ VGSRSPSMIS NDSETLEECM VCSDMKRDTL FGPCGHIATC 

       850        860        870        880        890        900 
SLCSPRVKKC LICKEQVQSR TKIEECVVCS DKKAAVLFQP CGHMCACENC ANLMKKCVQC 

       910        920        930        940        950        960 
RAVVERRVPF IMCCGGKSSE DATDDISSGN IPVLQKDKDN TNVNADVQKL QQQLQDIKEQ 

       970        980        990       1000 
TMCPVCLDRL KNMIFLCGHG TCQLCGDRMS ECPICRKAIE RRILLY

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta." Itoh M., Kim C.-H., Palardy G., Oda T., Jiang Y.-J., Maust D., Yeo S.-Y., Lorick K., Wright G.J., Ariza-McNaughton L., Weissman A.M., Lewis J., Chandrasekharappa S.C., Chitnis A.B. Dev. Cell 4:67-82(2003) [PubMed: 12530964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Yoo K.-W., Chitnis A., Kim C.-H. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NHLBI resequencing and genotyping service (RS&G) Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 195-1006. Tissue: Amygdala and Endometrial tumor.
[6]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 611-1006, TISSUE SPECIFICITY. Tissue: Brain.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 627-1006. Tissue: Placenta.
[8]	"Death-associated protein kinase expression in human temporal lobe epilepsy." Henshall D.C., Schindler C.K., So N.K., Lan J.-Q., Meller R., Simon R.P. Ann. Neurol. 55:485-494(2004) [PubMed: 15048887] [Abstract] Cited for: POSSIBLE SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	AY149908 mRNA. Translation: AAN75493.1. AY147849 mRNA. Translation: AAN18023.1. Frameshift. EF444995 Genomic DNA. Translation: ACA06016.1. BC022403 mRNA. Translation: AAH22403.1. Different initiation. BC110581 mRNA. Translation: AAI10582.1. BC110582 mRNA. Translation: AAI10583.1. CR749635 mRNA. Translation: CAH18429.1. AL713705 mRNA. Translation: CAD28502.1. AB037744 mRNA. Translation: BAA92561.1. AK075157 mRNA. Translation: BAC11439.1. Different initiation.
IPI	IPI00183054.
RefSeq	NP_065825.1.
UniGene	Hs.140903 Hs.658808
3D structure databases
HSSP	HSSP built from PDB template 1IKN based on UniProtKB P25963.
ModBase	Search...
Proteomic databases
PRIDE	Q86YT6.
Genome annotation databases
Ensembl	ENSG00000101752. Homo sapiens. [Contig view]
GeneID	57534.
KEGG	hsa:57534.
Organism-specific databases
GeneCards	GC18P017575.
H-InvDB	HIX0014360.
HGNC	HGNC:21086. MIB1.
HPA	HPA019100.
MIM	608677. gene.
PharmGKB	PA134862722.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q86YT6.
OMA	Q86YT6. NGRRGKX.
Gene expression databases
ArrayExpress	Q86YT6.
Bgee	Q86YT6.
CleanEx	HS_MIB1.
Family and domain databases
InterPro	IPR002110. ANK. IPR010606. MIB_HERC2. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. IPR000433. Znf_ZZ. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 8 hits. PF06701. MIB_HERC2. 2 hits. PF00097. zf-C3HC4. 1 hit. PF00569. ZZ. 1 hit. [Graphical view]
SMART	SM00248. ANK. 9 hits. SM00184. RING. 3 hits. SM00291. ZnF_ZZ. 1 hit. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 6 hits. PS51416. MIB_HERC2. 2 hits. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 3 hits. PS01357. ZF_ZZ_1. 1 hit. PS50135. ZF_ZZ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	63948.
SOURCE	Search...

Hide | Top

Entry information

Entry name

MIB1_HUMAN

Accession

Primary (citable) accession number: Q86YT6
Secondary accession number(s): B0YJ38

Q9P2M3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents