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Q86YT5 (S13A5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 13 member 5
Alternative name(s):
Na(+)/citrate cotransporter
Short name=NaCT
Sodium-coupled citrate transporter
Sodium-dependent citrate transporter
Gene names
Name:SLC13A5
Synonyms:NACT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity sodium/citrate cotransporter that mediates citrate entry into cells. The transport process is electrogenic; it is the trivalent form of citrate rather than the divalent form that is recognized as a substrate. May facilitate the utilization of circulating citrate for the generation of metabolic energy and for the synthesis of fatty acids and cholesterol. Ref.1

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed most predominantly in the liver, with moderate expression detectable in the brain and testis. Ref.1

Sequence similarities

Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86YT5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86YT5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     479-524: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568Solute carrier family 13 member 5
PRO_0000260101

Regions

Transmembrane13 – 3321Helical; Potential
Transmembrane53 – 7321Helical; Potential
Transmembrane80 – 10021Helical; Potential
Transmembrane124 – 14421Helical; Potential
Transmembrane215 – 23521Helical; Potential
Transmembrane252 – 27221Helical; Potential
Transmembrane311 – 33121Helical; Potential
Transmembrane353 – 37321Helical; Potential
Transmembrane406 – 42621Helical; Potential
Transmembrane439 – 45921Helical; Potential
Transmembrane487 – 50721Helical; Potential
Transmembrane528 – 54821Helical; Potential

Amino acid modifications

Glycosylation5621N-linked (GlcNAc...) Ref.5

Natural variations

Alternative sequence479 – 52446Missing in isoform 2.
VSP_043098

Experimental info

Sequence conflict2691W → R in BAD18766. Ref.2
Sequence conflict3761K → R in BAD18766. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: B8995E56618DECCB

FASTA56863,062
        10         20         30         40         50         60 
MASALSYVSK FKSFVILFVT PLLLLPLVIL MPAKFVRCAY VIILMAIYWC TEVIPLAVTS 

        70         80         90        100        110        120 
LMPVLLFPLF QILDSRQVCV QYMKDTNMLF LGGLIVAVAV ERWNLHKRIA LRTLLWVGAK 

       130        140        150        160        170        180 
PARLMLGFMG VTALLSMWIS NTATTAMMVP IVEAILQQME ATSAATEAGL ELVDKGKAKE 

       190        200        210        220        230        240 
LPGSQVIFEG PTLGQQEDQE RKRLCKAMTL CICYAASIGG TATLTGTGPN VVLLGQMNEL 

       250        260        270        280        290        300 
FPDSKDLVNF ASWFAFAFPN MLVMLLFAWL WLQFVYMRFN FKKSWGCGLE SKKNEKAALK 

       310        320        330        340        350        360 
VLQEEYRKLG PLSFAEINVL ICFFLLVILW FSRDPGFMPG WLTVAWVEGE TKYVSDATVA 

       370        380        390        400        410        420 
IFVATLLFIV PSQKPKFNFR SQTEEERKTP FYPPPLLDWK VTQEKVPWGI VLLLGGGFAL 

       430        440        450        460        470        480 
AKGSEASGLS VWMGKQMEPL HAVPPAAITL ILSLLVAVFT ECTSNVATTT LFLPIFASMS 

       490        500        510        520        530        540 
RSIGLNPLYI MLPCTLSASF AFMLPVATPP NAIVFTYGHL KVADMVKTGV IMNIIGVFCV 

       550        560 
FLAVNTWGRA IFDLDHFPDW ANVTHIET 

« Hide

Isoform 2 [UniParc].

Checksum: CD82CB02F6D574C7
Show »

FASTA52258,110

References

« Hide 'large scale' references
[1]"Human Na+ -coupled citrate transporter: primary structure, genomic organization, and transport function."
Inoue K., Zhuang L., Ganapathy V.
Biochem. Biophys. Res. Commun. 299:465-471(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[5]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-562.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY151833 mRNA. Translation: AAN86530.1.
AK172785 mRNA. Translation: BAD18766.1.
AC004706 Genomic DNA. No translation available.
BC104795 mRNA. Translation: AAI04796.1.
BC112151 mRNA. Translation: AAI12152.1.
BC143689 mRNA. Translation: AAI43690.1.
RefSeqNP_001137310.1. NM_001143838.2.
NP_808218.1. NM_177550.4.
UniGeneHs.399496.

3D structure databases

ProteinModelPortalQ86YT5.
SMRQ86YT5. Positions 22-549.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000293800.

Protein family/group databases

TCDB2.A.47.1.9. the divalent anion:na(+) symporter (dass) family.

PTM databases

PhosphoSiteQ86YT5.

Polymorphism databases

DMDM74714197.

Proteomic databases

PaxDbQ86YT5.
PRIDEQ86YT5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000433363; ENSP00000406220; ENSG00000141485. [Q86YT5-1]
ENST00000573648; ENSP00000459372; ENSG00000141485. [Q86YT5-2]
GeneID284111.
KEGGhsa:284111.
UCSCuc002gdj.3. human. [Q86YT5-1]
uc010vtf.2. human. [Q86YT5-2]

Organism-specific databases

CTD284111.
GeneCardsGC17M006588.
HGNCHGNC:23089. SLC13A5.
HPAHPA044343.
MIM608305. gene.
neXtProtNX_Q86YT5.
PharmGKBPA134950956.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0471.
HOGENOMHOG000278432.
HOVERGENHBG055339.
InParanoidQ86YT5.
KOK14445.
OMAYMRFNFK.
OrthoDBEOG7G1V5S.
PhylomeDBQ86YT5.
TreeFamTF312913.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_20633. Bile salt and organic anion SLC transporters.

Gene expression databases

ArrayExpressQ86YT5.
BgeeQ86YT5.
CleanExHS_SLC13A5.
GenevestigatorQ86YT5.

Family and domain databases

InterProIPR001898. Na/sul_symport.
[Graphical view]
PfamPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEPS01271. NA_SULFATE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi284111.
NextBio94549.
PROQ86YT5.
SOURCESearch...

Entry information

Entry nameS13A5_HUMAN
AccessionPrimary (citable) accession number: Q86YT5
Secondary accession number(s): B7ZLB4, Q6ZMG1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM