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Protein

Solute carrier family 13 member 5

Gene

SLC13A5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity sodium/citrate cotransporter that mediates citrate entry into cells. The transport process is electrogenic; it is the trivalent form of citrate rather than the divalent form that is recognized as a substrate. May facilitate the utilization of circulating citrate for the generation of metabolic energy and for the synthesis of fatty acids and cholesterol.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_20646. Sodium-coupled sulphate, di- and tri-carboxylate transporters.

Protein family/group databases

TCDBi2.A.47.1.9. the divalent anion:na(+) symporter (dass) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 13 member 5
Alternative name(s):
Na(+)/citrate cotransporter
Short name:
NaCT
Sodium-coupled citrate transporter
Sodium-dependent citrate transporter
Gene namesi
Name:SLC13A5
Synonyms:NACT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:23089. SLC13A5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence AnalysisAdd
BLAST
Transmembranei124 – 14421HelicalSequence AnalysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence AnalysisAdd
BLAST
Transmembranei252 – 27221HelicalSequence AnalysisAdd
BLAST
Transmembranei311 – 33121HelicalSequence AnalysisAdd
BLAST
Transmembranei353 – 37321HelicalSequence AnalysisAdd
BLAST
Transmembranei406 – 42621HelicalSequence AnalysisAdd
BLAST
Transmembranei439 – 45921HelicalSequence AnalysisAdd
BLAST
Transmembranei487 – 50721HelicalSequence AnalysisAdd
BLAST
Transmembranei528 – 54821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

Orphaneti1934. Early infantile epileptic encephalopathy.
PharmGKBiPA134950956.

Polymorphism and mutation databases

DMDMi74714197.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Solute carrier family 13 member 5PRO_0000260101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi562 – 5621N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ86YT5.
PaxDbiQ86YT5.
PRIDEiQ86YT5.

PTM databases

PhosphoSiteiQ86YT5.

Expressioni

Tissue specificityi

Expressed most predominantly in the liver, with moderate expression detectable in the brain and testis.1 Publication

Gene expression databases

BgeeiQ86YT5.
CleanExiHS_SLC13A5.
ExpressionAtlasiQ86YT5. baseline and differential.
GenevisibleiQ86YT5. HS.

Organism-specific databases

HPAiHPA044343.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000406220.

Structurei

3D structure databases

ProteinModelPortaliQ86YT5.
SMRiQ86YT5. Positions 41-275, 396-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0471.
GeneTreeiENSGT00550000074480.
HOGENOMiHOG000278432.
HOVERGENiHBG055339.
InParanoidiQ86YT5.
KOiK14445.
OMAiIHEIQVM.
OrthoDBiEOG7G1V5S.
PhylomeDBiQ86YT5.
TreeFamiTF312913.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEiPS01271. NA_SULFATE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YT5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASALSYVSK FKSFVILFVT PLLLLPLVIL MPAKFVRCAY VIILMAIYWC
60 70 80 90 100
TEVIPLAVTS LMPVLLFPLF QILDSRQVCV QYMKDTNMLF LGGLIVAVAV
110 120 130 140 150
ERWNLHKRIA LRTLLWVGAK PARLMLGFMG VTALLSMWIS NTATTAMMVP
160 170 180 190 200
IVEAILQQME ATSAATEAGL ELVDKGKAKE LPGSQVIFEG PTLGQQEDQE
210 220 230 240 250
RKRLCKAMTL CICYAASIGG TATLTGTGPN VVLLGQMNEL FPDSKDLVNF
260 270 280 290 300
ASWFAFAFPN MLVMLLFAWL WLQFVYMRFN FKKSWGCGLE SKKNEKAALK
310 320 330 340 350
VLQEEYRKLG PLSFAEINVL ICFFLLVILW FSRDPGFMPG WLTVAWVEGE
360 370 380 390 400
TKYVSDATVA IFVATLLFIV PSQKPKFNFR SQTEEERKTP FYPPPLLDWK
410 420 430 440 450
VTQEKVPWGI VLLLGGGFAL AKGSEASGLS VWMGKQMEPL HAVPPAAITL
460 470 480 490 500
ILSLLVAVFT ECTSNVATTT LFLPIFASMS RSIGLNPLYI MLPCTLSASF
510 520 530 540 550
AFMLPVATPP NAIVFTYGHL KVADMVKTGV IMNIIGVFCV FLAVNTWGRA
560
IFDLDHFPDW ANVTHIET
Length:568
Mass (Da):63,062
Last modified:June 1, 2003 - v1
Checksum:iB8995E56618DECCB
GO
Isoform 2 (identifier: Q86YT5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     479-524: Missing.

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):58,110
Checksum:iCD82CB02F6D574C7
GO
Isoform 3 (identifier: Q86YT5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-140: Missing.

Note: No experimental confirmation available.
Show »
Length:551
Mass (Da):61,210
Checksum:iB14F022B99048FF6
GO
Isoform 4 (identifier: Q86YT5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-77: Missing.

Note: No experimental confirmation available.
Show »
Length:525
Mass (Da):58,092
Checksum:iF2D93F70F424B588
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti269 – 2691W → R in BAD18766 (PubMed:14702039).Curated
Sequence conflicti330 – 3301W → R in BAH12628 (PubMed:14702039).Curated
Sequence conflicti376 – 3761K → R in BAD18766 (PubMed:14702039).Curated
Sequence conflicti475 – 4751I → V in BAH12628 (PubMed:14702039).Curated
Sequence conflicti548 – 5481G → E in BAH12628 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei35 – 7743Missing in isoform 4. 1 PublicationVSP_055652Add
BLAST
Alternative sequencei124 – 14017Missing in isoform 3. 1 PublicationVSP_054910Add
BLAST
Alternative sequencei479 – 52446Missing in isoform 2. 1 PublicationVSP_043098Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151833 mRNA. Translation: AAN86530.1.
AK172785 mRNA. Translation: BAD18766.1.
AK127797 mRNA. Translation: BAG54572.1.
AK297612 mRNA. Translation: BAH12628.1.
AC004706 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90292.1.
BC104795 mRNA. Translation: AAI04796.1.
BC112151 mRNA. Translation: AAI12152.1.
BC143689 mRNA. Translation: AAI43690.1.
CCDSiCCDS11079.1. [Q86YT5-1]
CCDS45593.1. [Q86YT5-2]
CCDS67136.1. [Q86YT5-4]
CCDS67137.1. [Q86YT5-3]
RefSeqiNP_001137310.1. NM_001143838.2. [Q86YT5-2]
NP_001271438.1. NM_001284509.1. [Q86YT5-3]
NP_001271439.1. NM_001284510.1. [Q86YT5-4]
NP_808218.1. NM_177550.4. [Q86YT5-1]
UniGeneiHs.399496.

Genome annotation databases

EnsembliENST00000293800; ENSP00000293800; ENSG00000141485. [Q86YT5-3]
ENST00000381074; ENSP00000370464; ENSG00000141485. [Q86YT5-4]
ENST00000433363; ENSP00000406220; ENSG00000141485.
ENST00000573648; ENSP00000459372; ENSG00000141485. [Q86YT5-2]
GeneIDi284111.
KEGGihsa:284111.
UCSCiuc002gdj.3. human. [Q86YT5-1]
uc002gdk.3. human.
uc010vtf.2. human. [Q86YT5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY151833 mRNA. Translation: AAN86530.1.
AK172785 mRNA. Translation: BAD18766.1.
AK127797 mRNA. Translation: BAG54572.1.
AK297612 mRNA. Translation: BAH12628.1.
AC004706 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90292.1.
BC104795 mRNA. Translation: AAI04796.1.
BC112151 mRNA. Translation: AAI12152.1.
BC143689 mRNA. Translation: AAI43690.1.
CCDSiCCDS11079.1. [Q86YT5-1]
CCDS45593.1. [Q86YT5-2]
CCDS67136.1. [Q86YT5-4]
CCDS67137.1. [Q86YT5-3]
RefSeqiNP_001137310.1. NM_001143838.2. [Q86YT5-2]
NP_001271438.1. NM_001284509.1. [Q86YT5-3]
NP_001271439.1. NM_001284510.1. [Q86YT5-4]
NP_808218.1. NM_177550.4. [Q86YT5-1]
UniGeneiHs.399496.

3D structure databases

ProteinModelPortaliQ86YT5.
SMRiQ86YT5. Positions 41-275, 396-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000406220.

Protein family/group databases

TCDBi2.A.47.1.9. the divalent anion:na(+) symporter (dass) family.

PTM databases

PhosphoSiteiQ86YT5.

Polymorphism and mutation databases

DMDMi74714197.

Proteomic databases

MaxQBiQ86YT5.
PaxDbiQ86YT5.
PRIDEiQ86YT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293800; ENSP00000293800; ENSG00000141485. [Q86YT5-3]
ENST00000381074; ENSP00000370464; ENSG00000141485. [Q86YT5-4]
ENST00000433363; ENSP00000406220; ENSG00000141485.
ENST00000573648; ENSP00000459372; ENSG00000141485. [Q86YT5-2]
GeneIDi284111.
KEGGihsa:284111.
UCSCiuc002gdj.3. human. [Q86YT5-1]
uc002gdk.3. human.
uc010vtf.2. human. [Q86YT5-2]

Organism-specific databases

CTDi284111.
GeneCardsiGC17M006588.
HGNCiHGNC:23089. SLC13A5.
HPAiHPA044343.
MIMi608305. gene.
neXtProtiNX_Q86YT5.
Orphaneti1934. Early infantile epileptic encephalopathy.
PharmGKBiPA134950956.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0471.
GeneTreeiENSGT00550000074480.
HOGENOMiHOG000278432.
HOVERGENiHBG055339.
InParanoidiQ86YT5.
KOiK14445.
OMAiIHEIQVM.
OrthoDBiEOG7G1V5S.
PhylomeDBiQ86YT5.
TreeFamiTF312913.

Enzyme and pathway databases

ReactomeiREACT_20646. Sodium-coupled sulphate, di- and tri-carboxylate transporters.

Miscellaneous databases

GenomeRNAii284111.
NextBioi35470627.
PROiQ86YT5.
SOURCEiSearch...

Gene expression databases

BgeeiQ86YT5.
CleanExiHS_SLC13A5.
ExpressionAtlasiQ86YT5. baseline and differential.
GenevisibleiQ86YT5. HS.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
[Graphical view]
PROSITEiPS01271. NA_SULFATE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human Na+ -coupled citrate transporter: primary structure, genomic organization, and transport function."
    Inoue K., Zhuang L., Ganapathy V.
    Biochem. Biophys. Res. Commun. 299:465-471(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
    Tissue: Brain.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  6. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-562.
    Tissue: Liver.

Entry informationi

Entry nameiS13A5_HUMAN
AccessioniPrimary (citable) accession number: Q86YT5
Secondary accession number(s): B3KXR0
, B7Z4P2, B7ZLB4, F8W7N2, Q6ZMG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.