Q86YT5 (S13A5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 13 member 5

Alternative name(s):
Na(+)/citrate cotransporter
Short name=NaCT
Sodium-coupled citrate transporter
Sodium-dependent citrate transporter

Gene names

Name:	SLC13A5
Synonyms:	NACT

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity sodium/citrate cotransporter that mediates citrate entry into cells. The transport process is electrogenic; it is the trivalent form of citrate rather than the divalent form that is recognized as a substrate. May facilitate the utilization of circulating citrate for the generation of metabolic energy and for the synthesis of fatty acids and cholesterol. Ref.1
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed most predominantly in the liver, with moderate expression detectable in the brain and testis. Ref.1
Sequence similarities	Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	succinate transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	citrate transmembrane transporter activity Inferred from electronic annotation. Source: Compara sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: Compara succinate transmembrane transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86YT5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86YT5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     479-524: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Solute carrier family 13 member 5		PRO_0000260101
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	80 – 100	21	Helical; Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Transmembrane	215 – 235	21	Helical; Potential
	Transmembrane	252 – 272	21	Helical; Potential
	Transmembrane	311 – 331	21	Helical; Potential
	Transmembrane	353 – 373	21	Helical; Potential
	Transmembrane	406 – 426	21	Helical; Potential
	Transmembrane	439 – 459	21	Helical; Potential
	Transmembrane	487 – 507	21	Helical; Potential
	Transmembrane	528 – 548	21	Helical; Potential
Amino acid modifications
	Glycosylation	562	1	N-linked (GlcNAc...) Ref.5
Natural variations
	Alternative sequence	479 – 524	46	Missing in isoform 2.		VSP_043098
Experimental info
	Sequence conflict	269	1	W → R in BAD18766. Ref.2
	Sequence conflict	376	1	K → R in BAD18766. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B8995E56618DECCB	FASTA	568	63,062
	10 20 30 40 50 60 MASALSYVSK FKSFVILFVT PLLLLPLVIL MPAKFVRCAY VIILMAIYWC TEVIPLAVTS 70 80 90 100 110 120 LMPVLLFPLF QILDSRQVCV QYMKDTNMLF LGGLIVAVAV ERWNLHKRIA LRTLLWVGAK 130 140 150 160 170 180 PARLMLGFMG VTALLSMWIS NTATTAMMVP IVEAILQQME ATSAATEAGL ELVDKGKAKE 190 200 210 220 230 240 LPGSQVIFEG PTLGQQEDQE RKRLCKAMTL CICYAASIGG TATLTGTGPN VVLLGQMNEL 250 260 270 280 290 300 FPDSKDLVNF ASWFAFAFPN MLVMLLFAWL WLQFVYMRFN FKKSWGCGLE SKKNEKAALK 310 320 330 340 350 360 VLQEEYRKLG PLSFAEINVL ICFFLLVILW FSRDPGFMPG WLTVAWVEGE TKYVSDATVA 370 380 390 400 410 420 IFVATLLFIV PSQKPKFNFR SQTEEERKTP FYPPPLLDWK VTQEKVPWGI VLLLGGGFAL 430 440 450 460 470 480 AKGSEASGLS VWMGKQMEPL HAVPPAAITL ILSLLVAVFT ECTSNVATTT LFLPIFASMS 490 500 510 520 530 540 RSIGLNPLYI MLPCTLSASF AFMLPVATPP NAIVFTYGHL KVADMVKTGV IMNIIGVFCV 550 560 FLAVNTWGRA IFDLDHFPDW ANVTHIET
	Isoform 2 [UniParc]. Checksum: CD82CB02F6D574C7	FASTA	522	58,110
	10 20 30 40 50 60 MASALSYVSK FKSFVILFVT PLLLLPLVIL MPAKFVRCAY VIILMAIYWC TEVIPLAVTS 70 80 90 100 110 120 LMPVLLFPLF QILDSRQVCV QYMKDTNMLF LGGLIVAVAV ERWNLHKRIA LRTLLWVGAK 130 140 150 160 170 180 PARLMLGFMG VTALLSMWIS NTATTAMMVP IVEAILQQME ATSAATEAGL ELVDKGKAKE 190 200 210 220 230 240 LPGSQVIFEG PTLGQQEDQE RKRLCKAMTL CICYAASIGG TATLTGTGPN VVLLGQMNEL 250 260 270 280 290 300 FPDSKDLVNF ASWFAFAFPN MLVMLLFAWL WLQFVYMRFN FKKSWGCGLE SKKNEKAALK 310 320 330 340 350 360 VLQEEYRKLG PLSFAEINVL ICFFLLVILW FSRDPGFMPG WLTVAWVEGE TKYVSDATVA 370 380 390 400 410 420 IFVATLLFIV PSQKPKFNFR SQTEEERKTP FYPPPLLDWK VTQEKVPWGI VLLLGGGFAL 430 440 450 460 470 480 AKGSEASGLS VWMGKQMEPL HAVPPAAITL ILSLLVAVFT ECTSNVATTT LFLPIFASMV 490 500 510 520 KTGVIMNIIG VFCVFLAVNT WGRAIFDLDH FPDWANVTHI ET

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human Na+ -coupled citrate transporter: primary structure, genomic organization, and transport function." Inoue K., Zhuang L., Ganapathy V. Biochem. Biophys. Res. Commun. 299:465-471(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-562, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY151833 mRNA. Translation: AAN86530.1. AK172785 mRNA. Translation: BAD18766.1. AC004706 Genomic DNA. No translation available. BC104795 mRNA. Translation: AAI04796.1. BC112151 mRNA. Translation: AAI12152.1. BC143689 mRNA. Translation: AAI43690.1.
IPI	IPI00329445. IPI00916961.
RefSeq	NP_001137310.1. NM_001143838.1. NP_808218.1. NM_177550.3.
UniGene	Hs.399496.
3D structure databases
ProteinModelPortal	Q86YT5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000293800.
Protein family/group databases
TCDB	2.A.47.1.9. divalent anion:Na+ symporter (DASS) family.
PTM databases
PhosphoSite	Q86YT5.
Polymorphism databases
DMDM	74714197.
Proteomic databases
PaxDb	Q86YT5.
PRIDE	Q86YT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000433363; ENSP00000406220; ENSG00000141485. ENST00000573648; ENSP00000459372; ENSG00000141485.
GeneID	284111.
KEGG	hsa:284111.
UCSC	uc002gdj.3. human.
Organism-specific databases
CTD	284111.
GeneCards	GC17M006588.
HGNC	HGNC:23089. SLC13A5.
HPA	HPA044343.
MIM	608305. gene.
neXtProt	NX_Q86YT5.
PharmGKB	PA134950956.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0471.
HOGENOM	HOG000278432.
HOVERGEN	HBG055339.
InParanoid	Q86YT5.
KO	K14445.
OMA	YMRFNFK.
OrthoDB	EOG4Q58P9.
PhylomeDB	Q86YT5.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_20633. Bile salt and organic anion SLC transporters.
Gene expression databases
ArrayExpress	Q86YT5.
Bgee	Q86YT5.
CleanEx	HS_SLC13A5.
Genevestigator	Q86YT5.
GermOnline	ENSG00000141485. Homo sapiens.
Family and domain databases
InterPro	IPR001898. Na/sul_symport. [Graphical view]
Pfam	PF00939. Na_sulph_symp. 1 hit. [Graphical view]
PROSITE	PS01271. NA_SULFATE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	284111.
NextBio	94549.
SOURCE	Search...

Entry information

Entry name

S13A5_HUMAN

Accession

Primary (citable) accession number: Q86YT5
Secondary accession number(s): B7ZLB4, Q6ZMG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.