Q86YS6 (RAB43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-43

Alternative name(s):
Ras-related protein Rab-41

Gene names

Name:	RAB43
Synonyms:	RAB41

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Cytoplasmic vesicle › phagosome. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Golgi apparatus. Golgi apparatus › trans-Golgi network membrane; Lipid-anchor By similarity. Golgi apparatus › trans-Golgi network. Note: Recruited to phagosomes containing S.aureus or M.tuberculosis. Ref.5 Ref.7 Ref.8
Tissue specificity	Widely expressed in brain, testis, lung, heart, ovary, colon, kidney, uterus and spleen but not in liver. Ref.1
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi organization Inferred from mutant phenotype Ref.6. Source: UniProtKB phagosome maturation Inferred from mutant phenotype Ref.8. Source: UniProtKB protein transport Inferred from electronic annotation. Source: InterPro retrograde transport, plasma membrane to Golgi Inferred from mutant phenotype Ref.5. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay Ref.5. Source: UniProtKB phagocytic vesicle Inferred from direct assay Ref.8. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86YS6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ULR0-1)

Also known as: ISY1-RAB43;

The sequence of this isoform can be found in the external entry Q9ULR0.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Based on a readthrough transcript which may produce a ISY1-RAB43 fusion protein. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 212

212

Ras-related protein Rab-43

PRO_0000244615

Regions

Nucleotide binding

25 – 32

8

GTP

Nucleotide binding

73 – 77

5

GTP By similarity

Nucleotide binding

131 – 134

4

GTP

Nucleotide binding

163 – 164

2

GTP

Motif

47 – 55

9

Effector region By similarity

Amino acid modifications

Modified residue

82

1

Phosphothreonine By similarity

Modified residue

212

1

Cysteine methyl ester By similarity

Lipidation

210

1

S-geranylgeranyl cysteine By similarity

Lipidation

212

1

S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis

32

1

T → N: Abolishes activity. Disrupts Golgi structure. Ref.6 Ref.7

Secondary structure

1

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212

Helix Strand Turn

Details...

Beta strand

17 – 25

9

Helix

31 – 40

10

Beta strand

55 – 62

8

Beta strand

65 – 72

8

Helix

78 – 80

3

Helix

81 – 88

8

Beta strand

92 – 99

8

Helix

103 – 107

5

Helix

109 – 119

11

Beta strand

125 – 131

7

Helix

136 – 138

3

Helix

143 – 152

10

Beta strand

156 – 160

5

Turn

163 – 166

4

Helix

169 – 183

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 087EE3802B7159C9	FASTA	212	23,339
	10 20 30 40 50 60 MAGPGPGPGD PDEQYDFLFK LVLVGDASVG KTCVVQRFKT GAFSERQGST IGVDFTMKTL 70 80 90 100 110 120 EIQGKRVKLQ IWDTAGQERF RTITQSYYRS ANGAILAYDI TKRSSFLSVP HWIEDVRKYA 130 140 150 160 170 180 GSNIVQLLIG NKSDLSELRE VSLAEAQSLA EHYDILCAIE TSAKDSSNVE EAFLRVATEL 190 200 210 IMRHGGPLFS EKSPDHIQLN SKDIGEGWGC GC
	Isoform 2 (ISY1-RAB43) [UniParc]. See Q9ULR0.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, expression pattern of a novel human RAB gene RAB41 and characterization of its intronless homolog RAB41P." Guo J.H., Chen L., Chen S., Liu X., Saiyin H., Deng Q., Zhuang Y., Wan B., Yu L., Zhao S.Y. DNA Seq. 14:431-435(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[5]	"Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways." Fuchs E., Haas A.K., Spooner R.A., Yoshimura S., Lord J.M., Barr F.A. J. Cell Biol. 177:1133-1143(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells." Haas A.K., Yoshimura S., Stephens D.J., Preisinger C., Fuchs E., Barr F.A. J. Cell Sci. 120:2997-3010(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF THR-32.
[7]	"Rab18 and Rab43 have key roles in ER-Golgi trafficking." Dejgaard S.Y., Murshid A., Erman A., Kizilay O., Verbich D., Lodge R., Dejgaard K., Ly-Hartig T.B., Pepperkok R., Simpson J.C., Presley J.F. J. Cell Sci. 121:2768-2781(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF THR-32, SUBCELLULAR LOCATION.
[8]	"Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes." Seto S., Tsujimura K., Koide Y. Traffic 12:407-420(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Crystal structure of human RAB43 in complex with GDP." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 9-190 IN COMPLEX WITH GDP.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY166852 mRNA. Translation: AAO17291.1.
AK291014 mRNA. Translation: BAF83703.1.
CH471052 Genomic DNA. Translation: EAW79283.1.
BC062319 mRNA. Translation: AAH62319.1.

IPI

IPI00329441.

RefSeq

NP_001191812.1. NM_001204883.1.
NP_001191813.1. NM_001204884.1.
NP_001191814.1. NM_001204885.1.
NP_001191815.1. NM_001204886.1.
NP_940892.1. NM_198490.2.

UniGene

Hs.381132.
Hs.546542.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HUP	X-ray	2.05	A/B	9-190	[»]

ProteinModelPortal

Q86YS6.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000319781.

PTM databases

PhosphoSite

Q86YS6.

Polymorphism databases

DMDM

74727944.

Proteomic databases

PaxDb

Q86YS6.

PRIDE

Q86YS6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000315150; ENSP00000319781; ENSG00000172780.
ENST00000393304; ENSP00000376981; ENSG00000172780.
ENST00000393305; ENSP00000376982; ENSG00000172780.
ENST00000393307; ENSP00000376984; ENSG00000172780.
ENST00000393308; ENSP00000376985; ENSG00000172780.

GeneID

339122.

KEGG

hsa:339122.

UCSC

uc003eln.2. human.

Organism-specific databases

CTD

339122.

GeneCards

GC03M128806.

HGNC

HGNC:19983. RAB43.

HPA

HPA046840.

neXtProt

NX_Q86YS6.

PharmGKB

PA134968262.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

InParanoid

Q86YS6.

KO

K07930.

OMA

LIGDCGT.

OrthoDB

EOG4WQ13N.

Gene expression databases

ArrayExpress

Q86YS6.

Bgee

Q86YS6.

CleanEx

HS_RAB41.
HS_RAB43.

Genevestigator

Q86YS6.

GermOnline

ENSG00000172780. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q86YS6.

GenomeRNAi

339122.

NextBio

97235.

Entry information

Entry name

RAB43_HUMAN

Accession

Primary (citable) accession number: Q86YS6
Secondary accession number(s): A8K4P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.