Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q86YS6

- RAB43_HUMAN

UniProt

Q86YS6 - RAB43_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ras-related protein Rab-43

Gene

RAB43

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The low intrinsic GTPase activity of RAB43 is activated by USP6NL. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for the structural integrity of the Golgi complex. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328GTP
Nucleotide bindingi73 – 775GTPBy similarity
Nucleotide bindingi131 – 1344GTP
Nucleotide bindingi163 – 1642GTP

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. Golgi organization Source: UniProtKB
  2. GTP catabolic process Source: UniProtKB
  3. phagosome maturation Source: UniProtKB
  4. protein transport Source: InterPro
  5. retrograde transport, plasma membrane to Golgi Source: UniProtKB
  6. small GTPase mediated signal transduction Source: InterPro
  7. virion assembly Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-43
Alternative name(s):
Ras-related protein Rab-41
Gene namesi
Name:RAB43
Synonyms:RAB41
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:19983. RAB43.

Subcellular locationi

Cytoplasmic vesiclephagosome. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Golgi apparatus. Golgi apparatustrans-Golgi network membrane By similarity; Lipid-anchor By similarity. Golgi apparatustrans-Golgi network
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. Golgi apparatus Source: UniProtKB
  3. membrane Source: UniProtKB-KW
  4. phagocytic vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321T → N: Abolishes activity. Disrupts Golgi structure. 2 Publications

Organism-specific databases

PharmGKBiPA134968262.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Ras-related protein Rab-43PRO_0000244615Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineBy similarity
Lipidationi210 – 2101S-geranylgeranyl cysteineBy similarity
Modified residuei212 – 2121Cysteine methyl esterBy similarity
Lipidationi212 – 2121S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ86YS6.
PaxDbiQ86YS6.
PRIDEiQ86YS6.

PTM databases

PhosphoSiteiQ86YS6.

Expressioni

Tissue specificityi

Widely expressed in brain, testis, lung, heart, ovary, colon, kidney, uterus and spleen but not in liver.1 Publication

Gene expression databases

BgeeiQ86YS6.
CleanExiHS_RAB41.
HS_RAB43.
ExpressionAtlasiQ86YS6. baseline.
GenevestigatoriQ86YS6.

Organism-specific databases

HPAiHPA046840.

Interactioni

Protein-protein interaction databases

BioGridi130830. 2 interactions.
STRINGi9606.ENSP00000319781.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi17 – 259
Helixi31 – 4010
Beta strandi55 – 628
Beta strandi65 – 728
Helixi78 – 803
Helixi81 – 888
Beta strandi92 – 998
Helixi103 – 1075
Helixi109 – 11911
Beta strandi125 – 1317
Helixi136 – 1383
Helixi143 – 15210
Beta strandi156 – 1605
Turni163 – 1664
Helixi169 – 18315

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HUPX-ray2.05A/B9-190[»]
ProteinModelPortaliQ86YS6.
SMRiQ86YS6. Positions 15-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86YS6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 559Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ86YS6.
KOiK07930.
OMAiKTCIVQR.
OrthoDBiEOG7NGQCK.
PhylomeDBiQ86YS6.
TreeFamiTF300097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q86YS6) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPGPGPGD PDEQYDFLFK LVLVGDASVG KTCVVQRFKT GAFSERQGST
60 70 80 90 100
IGVDFTMKTL EIQGKRVKLQ IWDTAGQERF RTITQSYYRS ANGAILAYDI
110 120 130 140 150
TKRSSFLSVP HWIEDVRKYA GSNIVQLLIG NKSDLSELRE VSLAEAQSLA
160 170 180 190 200
EHYDILCAIE TSAKDSSNVE EAFLRVATEL IMRHGGPLFS EKSPDHIQLN
210
SKDIGEGWGC GC
Length:212
Mass (Da):23,339
Last modified:June 1, 2003 - v1
Checksum:i087EE3802B7159C9
GO
Isoform 2 (identifier: Q9ULR0-1) [UniParc]FASTAAdd to Basket

Also known as: ISY1-RAB43

The sequence of this isoform can be found in the external entry Q9ULR0.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Based on a readthrough transcript which may produce a ISY1-RAB43 fusion protein. No experimental confirmation available.

Length:331
Mass (Da):37,567
GO
Isoform 3 (identifier: Q86YS6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-155: GNKSDLSELREVSLAEAQSLAEHYDI → EMQSCYVAQADLELLASSNPPASTSK
     156-212: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.

Show »
Length:155
Mass (Da):17,074
Checksum:iCC4EA3C8FD5E005D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei130 – 15526GNKSD…EHYDI → EMQSCYVAQADLELLASSNP PASTSK in isoform 3. CuratedVSP_054030Add
BLAST
Alternative sequencei156 – 21257Missing in isoform 3. CuratedVSP_054031Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY166852 mRNA. Translation: AAO17291.1.
AK291014 mRNA. Translation: BAF83703.1.
AC108673 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79283.1.
BC062319 mRNA. Translation: AAH62319.1.
CCDSiCCDS33850.1. [Q86YS6-1]
CCDS56275.1. [Q86YS6-2]
RefSeqiNP_001191812.1. NM_001204883.1. [Q86YS6-1]
NP_001191813.1. NM_001204884.1. [Q86YS6-1]
NP_001191814.1. NM_001204885.1. [Q86YS6-1]
NP_001191815.1. NM_001204886.1. [Q86YS6-1]
NP_001191816.1. NM_001204887.1. [Q86YS6-2]
NP_940892.1. NM_198490.2. [Q86YS6-1]
UniGeneiHs.381132.
Hs.512661.
Hs.546542.

Genome annotation databases

EnsembliENST00000315150; ENSP00000319781; ENSG00000172780. [Q86YS6-1]
ENST00000393304; ENSP00000376981; ENSG00000172780. [Q86YS6-1]
ENST00000393305; ENSP00000376982; ENSG00000172780. [Q86YS6-1]
ENST00000393307; ENSP00000376984; ENSG00000172780. [Q86YS6-1]
ENST00000393308; ENSP00000376985; ENSG00000172780. [Q86YS6-1]
ENST00000476465; ENSP00000427632; ENSG00000172780. [Q86YS6-2]
ENST00000615093; ENSP00000481399; ENSG00000172780. [Q86YS6-2]
GeneIDi339122.
KEGGihsa:339122.
UCSCiuc003eln.2. human. [Q86YS6-1]

Polymorphism databases

DMDMi74727944.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY166852 mRNA. Translation: AAO17291.1 .
AK291014 mRNA. Translation: BAF83703.1 .
AC108673 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW79283.1 .
BC062319 mRNA. Translation: AAH62319.1 .
CCDSi CCDS33850.1. [Q86YS6-1 ]
CCDS56275.1. [Q86YS6-2 ]
RefSeqi NP_001191812.1. NM_001204883.1. [Q86YS6-1 ]
NP_001191813.1. NM_001204884.1. [Q86YS6-1 ]
NP_001191814.1. NM_001204885.1. [Q86YS6-1 ]
NP_001191815.1. NM_001204886.1. [Q86YS6-1 ]
NP_001191816.1. NM_001204887.1. [Q86YS6-2 ]
NP_940892.1. NM_198490.2. [Q86YS6-1 ]
UniGenei Hs.381132.
Hs.512661.
Hs.546542.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HUP X-ray 2.05 A/B 9-190 [» ]
ProteinModelPortali Q86YS6.
SMRi Q86YS6. Positions 15-186.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 130830. 2 interactions.
STRINGi 9606.ENSP00000319781.

PTM databases

PhosphoSitei Q86YS6.

Polymorphism databases

DMDMi 74727944.

Proteomic databases

MaxQBi Q86YS6.
PaxDbi Q86YS6.
PRIDEi Q86YS6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000315150 ; ENSP00000319781 ; ENSG00000172780 . [Q86YS6-1 ]
ENST00000393304 ; ENSP00000376981 ; ENSG00000172780 . [Q86YS6-1 ]
ENST00000393305 ; ENSP00000376982 ; ENSG00000172780 . [Q86YS6-1 ]
ENST00000393307 ; ENSP00000376984 ; ENSG00000172780 . [Q86YS6-1 ]
ENST00000393308 ; ENSP00000376985 ; ENSG00000172780 . [Q86YS6-1 ]
ENST00000476465 ; ENSP00000427632 ; ENSG00000172780 . [Q86YS6-2 ]
ENST00000615093 ; ENSP00000481399 ; ENSG00000172780 . [Q86YS6-2 ]
GeneIDi 339122.
KEGGi hsa:339122.
UCSCi uc003eln.2. human. [Q86YS6-1 ]

Organism-specific databases

CTDi 339122.
GeneCardsi GC03M128806.
HGNCi HGNC:19983. RAB43.
HPAi HPA046840.
neXtProti NX_Q86YS6.
PharmGKBi PA134968262.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118841.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi Q86YS6.
KOi K07930.
OMAi KTCIVQR.
OrthoDBi EOG7NGQCK.
PhylomeDBi Q86YS6.
TreeFami TF300097.

Miscellaneous databases

EvolutionaryTracei Q86YS6.
GenomeRNAii 339122.
NextBioi 97235.
PROi Q86YS6.

Gene expression databases

Bgeei Q86YS6.
CleanExi HS_RAB41.
HS_RAB43.
ExpressionAtlasi Q86YS6. baseline.
Genevestigatori Q86YS6.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, expression pattern of a novel human RAB gene RAB41 and characterization of its intronless homolog RAB41P."
    Guo J.H., Chen L., Chen S., Liu X., Saiyin H., Deng Q., Zhuang Y., Wan B., Yu L., Zhao S.Y.
    DNA Seq. 14:431-435(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  6. "Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways."
    Fuchs E., Haas A.K., Spooner R.A., Yoshimura S., Lord J.M., Barr F.A.
    J. Cell Biol. 177:1133-1143(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Analysis of GTPase-activating proteins: Rab1 and Rab43 are key Rabs required to maintain a functional Golgi complex in human cells."
    Haas A.K., Yoshimura S., Stephens D.J., Preisinger C., Fuchs E., Barr F.A.
    J. Cell Sci. 120:2997-3010(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF THR-32.
  8. Cited for: FUNCTION, MUTAGENESIS OF THR-32, SUBCELLULAR LOCATION.
  9. "Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes."
    Seto S., Tsujimura K., Koide Y.
    Traffic 12:407-420(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "Crystal structure of human RAB43 in complex with GDP."
    Structural genomics consortium (SGC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) OF 9-190 IN COMPLEX WITH GDP.

Entry informationi

Entry nameiRAB43_HUMAN
AccessioniPrimary (citable) accession number: Q86YS6
Secondary accession number(s): A8K4P9, E9PBQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3