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Protein

Rab11 family-interacting protein 4

Gene

RAB11FIP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. In case of infection by HCMV (human cytomegalovirus), may participate in egress of the virus out of nucleus; this function is independent of ARF6.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi62 – 73PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: UniProtKB
  • calcium ion binding Source: InterPro
  • protein homodimerization activity Source: UniProtKB
  • Rab GTPase binding Source: UniProtKB

GO - Biological processi

  • cytokinesis Source: UniProtKB
  • neural retina development Source: Ensembl
  • positive regulation of G1 to G0 transition Source: Ensembl
  • transport Source: UniProtKB-KW
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Rab11 family-interacting protein 4
Short name:
FIP4-Rab11
Short name:
Rab11-FIP4
Alternative name(s):
Arfophilin-2
Gene namesi
Name:RAB11FIP4
Synonyms:ARFO2, KIAA1821
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:30267. RAB11FIP4.

Subcellular locationi

GO - Cellular componenti

  • cleavage furrow Source: UniProtKB
  • endocytic vesicle Source: UniProtKB
  • endosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • microtubule organizing center Source: UniProtKB-SubCell
  • midbody Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
  • recycling endosome membrane Source: UniProtKB
  • spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi627D → A: Abolishes Rab11-binding. 1 Publication1

Organism-specific databases

DisGeNETi84440.
OpenTargetsiENSG00000131242.
PharmGKBiPA134978007.

Polymorphism and mutation databases

BioMutaiRAB11FIP4.
DMDMi67472134.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000738801 – 637Rab11 family-interacting protein 4Add BLAST637

Proteomic databases

EPDiQ86YS3.
MaxQBiQ86YS3.
PaxDbiQ86YS3.
PeptideAtlasiQ86YS3.
PRIDEiQ86YS3.

PTM databases

iPTMnetiQ86YS3.
PhosphoSitePlusiQ86YS3.

Expressioni

Tissue specificityi

Present at high level in testis (at protein level). Weakly expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSG00000131242.
CleanExiHS_RAB11FIP4.
ExpressionAtlasiQ86YS3. baseline and differential.
GenevisibleiQ86YS3. HS.

Organism-specific databases

HPAiHPA021595.

Interactioni

Subunit structurei

Homodimer. Forms a complex with Rab11 (RAB11A or RAB11B) and ARF6. Interacts with RAB11A; the interaction is direct. Forms a heterooligomeric complex with RAB11FIP2, RAB11FIP3 and RAB11FIP5. Interacts with HCMV (human cytomegalovirus) gM/UL100. Interacts with ECM29.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Arf6P623312EBI-949727,EBI-988682From a different organism.

GO - Molecular functioni

  • ADP-ribosylation factor binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • Rab GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124079. 16 interactors.
DIPiDIP-47495N.
IntActiQ86YS3. 4 interactors.
MINTiMINT-2877094.
STRINGi9606.ENSP00000312837.

Structurei

3D structure databases

ProteinModelPortaliQ86YS3.
SMRiQ86YS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 84EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini574 – 636FIP-RBDPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 637Necessary for interaction with RAB11A, subcellular location, homo- or heterooligomerizationAdd BLAST556

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili280 – 617Sequence analysisAdd BLAST338

Domaini

The RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B).By similarity

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 FIP-RBD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0982. Eukaryota.
ENOG4111K32. LUCA.
GeneTreeiENSGT00440000033742.
HOGENOMiHOG000063670.
HOVERGENiHBG054059.
InParanoidiQ86YS3.
KOiK12485.
OMAiGRQLMHS.
OrthoDBiEOG091G0421.
PhylomeDBiQ86YS3.
TreeFamiTF327221.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PfamiPF09457. RBD-FIP. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
PS51511. FIP_RBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGGAGWSGA PAALLRSVRR LREVFEVCGR DPDGFLRVER VAALGLRFGQ
60 70 80 90 100
GEEVEKLVKY LDPNDLGRIN FKDFCRGVFA MKGCEELLKD VLSVESAGTL
110 120 130 140 150
PCAPEIPDCV EQGSEVTGPT FADGELIPRE PGFFPEDEEE AMTLAPPEGP
160 170 180 190 200
QELYTDSPME STQSLEGSVG SPAEKDGGLG GLFLPEDKSL VHTPSMTTSD
210 220 230 240 250
LSTHSTTSLI SNEEQFEDYG EGDDVDCAPS SPCPDDETRT NVYSDLGSSV
260 270 280 290 300
SSSAGQTPRK MRHVYNSELL DVYCSQCCKK INLLNDLEAR LKNLKANSPN
310 320 330 340 350
RKISSTAFGR QLMHSSNFSS SNGSTEDLFR DSIDSCDNDI TEKVSFLEKK
360 370 380 390 400
VTELENDSLT NGDLKSKLKQ ENTQLVHRVH ELEEMVKDQE TTAEQALEEE
410 420 430 440 450
ARRHREAYGK LEREKATEVE LLNARVQQLE EENTELRTTV TRLKSQTEKL
460 470 480 490 500
DEERQRMSDR LEDTSLRLKD EMDLYKRMMD KLRQNRLEFQ KEREATQELI
510 520 530 540 550
EDLRKELEHL QMYKLDCERP GRGRSASSGL GEFNARAREV ELEHEVKRLK
560 570 580 590 600
QENYKLRDQN DDLNGQILSL SLYEAKNLFA AQTKAQSLAA EIDTASRDEL
610 620 630
MEALKEQEEI NFRLRQYMDK IILAILDHNP SILEIKH
Length:637
Mass (Da):71,928
Last modified:June 1, 2003 - v1
Checksum:i8A5CDB9799383342
GO
Isoform 2 (identifier: Q86YS3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.
     103-111: APEIPDCVE → MRTPPALGS

Show »
Length:535
Mass (Da):60,771
Checksum:i7ECB4806726B7D5D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti417T → I in BAC05045 (PubMed:14702039).Curated1
Sequence conflicti518E → G in BAC05045 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0137241 – 102Missing in isoform 2. 1 PublicationAdd BLAST102
Alternative sequenceiVSP_013725103 – 111APEIPDCVE → MRTPPALGS in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY169244 mRNA. Translation: AAO21970.1.
AK097424 mRNA. Translation: BAC05045.1.
BC093914 mRNA. Translation: AAH93914.1.
BC101517 mRNA. Translation: AAI01518.1.
AJ314646 mRNA. Translation: CAC44535.1.
AB058724 mRNA. Translation: BAB47450.1.
AL831830 mRNA. Translation: CAD38543.1.
CCDSiCCDS11267.1. [Q86YS3-1]
CCDS76985.1. [Q86YS3-2]
RefSeqiNP_001290471.2. NM_001303542.2. [Q86YS3-2]
NP_116321.2. NM_032932.5. [Q86YS3-1]
UniGeneiHs.406788.

Genome annotation databases

EnsembliENST00000394744; ENSP00000378227; ENSG00000131242. [Q86YS3-2]
ENST00000621161; ENSP00000482620; ENSG00000131242. [Q86YS3-1]
GeneIDi84440.
KEGGihsa:84440.
UCSCiuc032fao.2. human. [Q86YS3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY169244 mRNA. Translation: AAO21970.1.
AK097424 mRNA. Translation: BAC05045.1.
BC093914 mRNA. Translation: AAH93914.1.
BC101517 mRNA. Translation: AAI01518.1.
AJ314646 mRNA. Translation: CAC44535.1.
AB058724 mRNA. Translation: BAB47450.1.
AL831830 mRNA. Translation: CAD38543.1.
CCDSiCCDS11267.1. [Q86YS3-1]
CCDS76985.1. [Q86YS3-2]
RefSeqiNP_001290471.2. NM_001303542.2. [Q86YS3-2]
NP_116321.2. NM_032932.5. [Q86YS3-1]
UniGeneiHs.406788.

3D structure databases

ProteinModelPortaliQ86YS3.
SMRiQ86YS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124079. 16 interactors.
DIPiDIP-47495N.
IntActiQ86YS3. 4 interactors.
MINTiMINT-2877094.
STRINGi9606.ENSP00000312837.

PTM databases

iPTMnetiQ86YS3.
PhosphoSitePlusiQ86YS3.

Polymorphism and mutation databases

BioMutaiRAB11FIP4.
DMDMi67472134.

Proteomic databases

EPDiQ86YS3.
MaxQBiQ86YS3.
PaxDbiQ86YS3.
PeptideAtlasiQ86YS3.
PRIDEiQ86YS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394744; ENSP00000378227; ENSG00000131242. [Q86YS3-2]
ENST00000621161; ENSP00000482620; ENSG00000131242. [Q86YS3-1]
GeneIDi84440.
KEGGihsa:84440.
UCSCiuc032fao.2. human. [Q86YS3-1]

Organism-specific databases

CTDi84440.
DisGeNETi84440.
GeneCardsiRAB11FIP4.
HGNCiHGNC:30267. RAB11FIP4.
HPAiHPA021595.
MIMi611999. gene.
neXtProtiNX_Q86YS3.
OpenTargetsiENSG00000131242.
PharmGKBiPA134978007.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0982. Eukaryota.
ENOG4111K32. LUCA.
GeneTreeiENSGT00440000033742.
HOGENOMiHOG000063670.
HOVERGENiHBG054059.
InParanoidiQ86YS3.
KOiK12485.
OMAiGRQLMHS.
OrthoDBiEOG091G0421.
PhylomeDBiQ86YS3.
TreeFamiTF327221.

Miscellaneous databases

ChiTaRSiRAB11FIP4. human.
GeneWikiiRAB11FIP4.
GenomeRNAii84440.
PROiQ86YS3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131242.
CleanExiHS_RAB11FIP4.
ExpressionAtlasiQ86YS3. baseline and differential.
GenevisibleiQ86YS3. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PfamiPF09457. RBD-FIP. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 1 hit.
PS51511. FIP_RBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFIP4_HUMAN
AccessioniPrimary (citable) accession number: Q86YS3
Secondary accession number(s): Q52LI1
, Q8N829, Q8NDT7, Q969D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.