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Protein

G-protein-signaling modulator 1

Gene

GPSM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.2 Publications

GO - Molecular functioni

  • GDP-dissociation inhibitor activity Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • negative regulation of GTPase activity Source: UniProtKB
  • negative regulation of guanyl-nucleotide exchange factor activity Source: UniProtKB
  • nervous system development Source: UniProtKB-KW
  • positive regulation of macroautophagy Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-HSA-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein-signaling modulator 1
Alternative name(s):
Activator of G-protein signaling 3
Gene namesi
Name:GPSM1
Synonyms:AGS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000160360.11
HGNCiHGNC:17858 GPSM1
MIMi609491 gene
neXtProtiNX_Q86YR5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi26086
OpenTargetsiENSG00000160360
PharmGKBiPA134986171

Polymorphism and mutation databases

DMDMi294862435

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524021 – 675G-protein-signaling modulator 1Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei413PhosphoserineCombined sources1
Modified residuei421Omega-N-methylarginineBy similarity1
Modified residuei445PhosphoserineCombined sources1
Modified residuei469PhosphoserineCombined sources1
Modified residuei471PhosphoserineCombined sources1
Modified residuei492PhosphoserineCombined sources1
Modified residuei493PhosphoserineCombined sources1
Modified residuei545PhosphoserineBy similarity1
Modified residuei569PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation regulates interaction with G(i/o) alpha.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ86YR5
MaxQBiQ86YR5
PaxDbiQ86YR5
PeptideAtlasiQ86YR5
PRIDEiQ86YR5

PTM databases

iPTMnetiQ86YR5
PhosphoSitePlusiQ86YR5

Expressioni

Tissue specificityi

Expressed in intestinal cells.1 Publication

Gene expression databases

BgeeiENSG00000160360
CleanExiHS_GPSM1
ExpressionAtlasiQ86YR5 baseline and differential
GenevisibleiQ86YR5 HS

Organism-specific databases

HPAiHPA042199

Interactioni

Subunit structurei

Interacts with GNAI1, GNAI2 and GNAI3 preferentially in their GDP-bound state. May also interact with GNAO1. Interacts with STK11/LKB1 and MACF1 (By similarity). Interacts with INSC/inscuteable and FRMPD1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KANK2Q63ZY33EBI-10261098,EBI-2556193

Protein-protein interaction databases

BioGridi117539, 26 interactors
IntActiQ86YR5, 7 interactors
STRINGi9606.ENSP00000392828

Structurei

3D structure databases

ProteinModelPortaliQ86YR5
SMRiQ86YR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 61TPR 1Add BLAST34
Repeati66 – 99TPR 2Add BLAST34
Repeati106 – 139TPR 3Add BLAST34
Repeati146 – 181TPR 4Add BLAST36
Repeati183 – 202TPR 5Add BLAST20
Repeati209 – 242TPR 6Add BLAST34
Repeati249 – 282TPR 7Add BLAST34
Repeati289 – 322TPR 8Add BLAST34
Repeati329 – 362TPR 9Add BLAST34
Domaini495 – 517GoLoco 1PROSITE-ProRule annotationAdd BLAST23
Domaini548 – 570GoLoco 2PROSITE-ProRule annotationAdd BLAST23
Domaini596 – 618GoLoco 3PROSITE-ProRule annotationAdd BLAST23
Domaini630 – 652GoLoco 4PROSITE-ProRule annotationAdd BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 509Mediates association with membranesBy similarityAdd BLAST509
Regioni364 – 487Interaction with STK11/LKB1By similarityAdd BLAST124

Domaini

The GoLoco domains mediate interaction with G(i/o) alpha (By similarity). The GoLoco domains are essential for the GDI activity toward G(i/o) alpha.By similarity

Sequence similaritiesi

Belongs to the GPSM family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1130 Eukaryota
ENOG410XP6N LUCA
GeneTreeiENSGT00530000063126
HOGENOMiHOG000231543
HOVERGENiHBG051823
InParanoidiQ86YR5
KOiK15839
PhylomeDBiQ86YR5
TreeFamiTF328344

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR003109 GoLoco_motif
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF02188 GoLoco, 4 hits
SMARTiView protein in SMART
SM00390 GoLoco, 4 hits
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50877 GOLOCO, 4 hits
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 2 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YR5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPAPPVAD ELPGPAARRL YSRMEASCLE LALEGERLCK AGDFKTGVAF
60 70 80 90 100
FEAAVQVGTE DLKTLSAIYS QLGNAYFYLK EHGRALEYHK HDLLLARTIG
110 120 130 140 150
DRMGEAKASG NLGNTLKVLG RFDEAAVCCQ RHLSIAQEQG DKVGEARALY
160 170 180 190 200
NIGNVYHAKG KQLSWNAANA TQDPGHLPPD VRETLCKASE FYERNLSLVK
210 220 230 240 250
ELGDRAAQGR AYGNLGNTHY LLGNFTEATT FHKERLAIAK EFGDKAAERR
260 270 280 290 300
AYSNLGNAHV FLGRFDVAAE YYKKTLQLSR QLRDQAVEAQ ACYSLGNTYT
310 320 330 340 350
LLQDYERAAE YHLRHLLIAQ ELADRVGEGR ACWSLGNAYV SMGRPAQALT
360 370 380 390 400
FAKKHLQISQ EIGDRHGELT ARMNVAQLQL VLGRLTSPAA SEKPDLAGYE
410 420 430 440 450
AQGARPKRTQ RLSAETWDLL RLPLEREQNG DSHHSGDWRG PSRDSLPLPV
460 470 480 490 500
RSRKYQEGPD AERRPREGSH SPLDSADVRV HVPRTSIPRA PSSDEECFFD
510 520 530 540 550
LLTKFQSSRM DDQRCPLDDG QAGAAEATAA PTLEDRIAQP SMTASPQTEE
560 570 580 590 600
FFDLIASSQS RRLDDQRASV GSLPGLRITH SNAGHLRGHG EPQEPGDDFF
610 620 630 640 650
NMLIKYQSSR IDDQRCPPPD VLPRGPTMPD EDFFSLIQRV QAKRMDEQRV
660 670
DLAGGPEQGA GGPPEPQQQC QPGAS
Length:675
Mass (Da):74,510
Last modified:April 20, 2010 - v2
Checksum:i27EB86667EEC1951
GO
Isoform 2 (identifier: Q86YR5-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-509: Missing.

Note: Minor isoform.
Show »
Length:166
Mass (Da):18,024
Checksum:iB0009DE5201BD2BD
GO
Isoform 3 (identifier: Q86YR5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-457: ARPKRTQRLS...LPVRSRKYQE → EFQGCGGVLL...FFLASGTAQA
     458-675: Missing.

Note: No experimental confirmation available.
Show »
Length:457
Mass (Da):49,685
Checksum:i4493B11B5D9986DC
GO
Isoform 4 (identifier: Q86YR5-4) [UniParc]FASTAAdd to basket
Also known as: FL

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: Major isoform.
Show »
Length:652
Mass (Da):72,136
Checksum:iFF690E5EC1999611
GO

Sequence cautioni

The sequence AAH09979 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH17353 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB55951 differs from that shown. Reason: Frameshift at position 657.Curated
The sequence CAI19339 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0209371 – 509Missing in isoform 2. 1 PublicationAdd BLAST509
Alternative sequenceiVSP_0390281 – 23Missing in isoform 4. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_020938404 – 457ARPKR…RKYQE → EFQGCGGVLLPTGTDRIRSC GGVGSRPGQHGGGGSRQKMA PTSQFFLASGTAQA in isoform 3. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_020939458 – 675Missing in isoform 3. 1 PublicationAdd BLAST218

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY173053 mRNA Translation: AAO17260.1
BX649589 Genomic DNA Translation: CAI19338.1
BX649589 Genomic DNA Translation: CAI19339.1 Sequence problems.
BX649589 Genomic DNA Translation: CAM27654.1
CH471090 Genomic DNA Translation: EAW88216.1
BC009979 mRNA Translation: AAH09979.1 Different initiation.
BC017353 mRNA Translation: AAH17353.1 Different initiation.
BC048343 mRNA Translation: AAH48343.1
AL117478 mRNA Translation: CAB55951.1 Frameshift.
CCDSiCCDS48055.1 [Q86YR5-1]
CCDS48056.1 [Q86YR5-2]
CCDS6996.2 [Q86YR5-3]
PIRiT17261
RefSeqiNP_001139110.2, NM_001145638.2
NP_001139111.1, NM_001145639.1 [Q86YR5-2]
NP_001186932.1, NM_001200003.1 [Q86YR5-2]
NP_056412.5, NM_015597.5
XP_016870088.1, XM_017014599.1 [Q86YR5-2]
UniGeneiHs.239370

Genome annotation databases

EnsembliENST00000291775; ENSP00000291775; ENSG00000160360 [Q86YR5-2]
ENST00000392944; ENSP00000376673; ENSG00000160360 [Q86YR5-2]
ENST00000429455; ENSP00000390705; ENSG00000160360 [Q86YR5-2]
GeneIDi26086
KEGGihsa:26086
UCSCiuc004che.3 human [Q86YR5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGPSM1_HUMAN
AccessioniPrimary (citable) accession number: Q86YR5
Secondary accession number(s): A9Z1X4
, B1B0W3, Q86SR5, Q969T1, Q9UFS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: April 20, 2010
Last modified: April 25, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health