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Protein

G-protein-signaling modulator 1

Gene

GPSM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-HSA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein-signaling modulator 1
Alternative name(s):
Activator of G-protein signaling 3
Gene namesi
Name:GPSM1
Synonyms:AGS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:17858. GPSM1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi26086.
OpenTargetsiENSG00000160360.
PharmGKBiPA134986171.

Polymorphism and mutation databases

DMDMi294862435.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524021 – 675G-protein-signaling modulator 1Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei413PhosphoserineCombined sources1
Modified residuei421Omega-N-methylarginineBy similarity1
Modified residuei445PhosphoserineCombined sources1
Modified residuei469PhosphoserineCombined sources1
Modified residuei471PhosphoserineCombined sources1
Modified residuei492PhosphoserineCombined sources1
Modified residuei493PhosphoserineCombined sources1
Modified residuei545PhosphoserineBy similarity1
Modified residuei569PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation regulates interaction with G(i/o) alpha.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ86YR5.
MaxQBiQ86YR5.
PaxDbiQ86YR5.
PeptideAtlasiQ86YR5.
PRIDEiQ86YR5.

PTM databases

iPTMnetiQ86YR5.
PhosphoSitePlusiQ86YR5.

Expressioni

Tissue specificityi

Expressed in intestinal cells.1 Publication

Gene expression databases

BgeeiENSG00000160360.
CleanExiHS_GPSM1.
ExpressionAtlasiQ86YR5. baseline and differential.
GenevisibleiQ86YR5. HS.

Organism-specific databases

HPAiHPA042199.

Interactioni

Subunit structurei

Interacts with GNAI1, GNAI2 and GNAI3 preferentially in their GDP-bound state. May also interact with GNAO1. Interacts with STK11/LKB1 and MACF1 (By similarity). Interacts with INSC/inscuteable and FRMPD1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KANK2Q63ZY33EBI-10261098,EBI-2556193

Protein-protein interaction databases

BioGridi117539. 26 interactors.
IntActiQ86YR5. 7 interactors.
MINTiMINT-1453185.
STRINGi9606.ENSP00000392828.

Structurei

3D structure databases

ProteinModelPortaliQ86YR5.
SMRiQ86YR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 61TPR 1Add BLAST34
Repeati66 – 99TPR 2Add BLAST34
Repeati106 – 139TPR 3Add BLAST34
Repeati146 – 181TPR 4Add BLAST36
Repeati183 – 202TPR 5Add BLAST20
Repeati209 – 242TPR 6Add BLAST34
Repeati249 – 282TPR 7Add BLAST34
Repeati289 – 322TPR 8Add BLAST34
Repeati329 – 362TPR 9Add BLAST34
Domaini495 – 517GoLoco 1PROSITE-ProRule annotationAdd BLAST23
Domaini548 – 570GoLoco 2PROSITE-ProRule annotationAdd BLAST23
Domaini596 – 618GoLoco 3PROSITE-ProRule annotationAdd BLAST23
Domaini630 – 652GoLoco 4PROSITE-ProRule annotationAdd BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 509Mediates association with membranesBy similarityAdd BLAST509
Regioni364 – 487Interaction with STK11/LKB1By similarityAdd BLAST124

Domaini

The GoLoco domains mediate interaction with G(i/o) alpha (By similarity). The GoLoco domains are essential for the GDI activity toward G(i/o) alpha.By similarity

Sequence similaritiesi

Belongs to the GPSM family.Curated
Contains 4 GoLoco domains.PROSITE-ProRule annotation
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1130. Eukaryota.
ENOG410XP6N. LUCA.
GeneTreeiENSGT00530000063126.
HOGENOMiHOG000231543.
HOVERGENiHBG051823.
InParanoidiQ86YR5.
KOiK15839.
PhylomeDBiQ86YR5.
TreeFamiTF328344.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024804. G_prot_signal_mod_1.
IPR003109. GoLoco_motif.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10098:SF36. PTHR10098:SF36. 1 hit.
PfamiPF02188. GoLoco. 4 hits.
[Graphical view]
SMARTiSM00390. GoLoco. 4 hits.
SM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50877. GOLOCO. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YR5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPAPPVAD ELPGPAARRL YSRMEASCLE LALEGERLCK AGDFKTGVAF
60 70 80 90 100
FEAAVQVGTE DLKTLSAIYS QLGNAYFYLK EHGRALEYHK HDLLLARTIG
110 120 130 140 150
DRMGEAKASG NLGNTLKVLG RFDEAAVCCQ RHLSIAQEQG DKVGEARALY
160 170 180 190 200
NIGNVYHAKG KQLSWNAANA TQDPGHLPPD VRETLCKASE FYERNLSLVK
210 220 230 240 250
ELGDRAAQGR AYGNLGNTHY LLGNFTEATT FHKERLAIAK EFGDKAAERR
260 270 280 290 300
AYSNLGNAHV FLGRFDVAAE YYKKTLQLSR QLRDQAVEAQ ACYSLGNTYT
310 320 330 340 350
LLQDYERAAE YHLRHLLIAQ ELADRVGEGR ACWSLGNAYV SMGRPAQALT
360 370 380 390 400
FAKKHLQISQ EIGDRHGELT ARMNVAQLQL VLGRLTSPAA SEKPDLAGYE
410 420 430 440 450
AQGARPKRTQ RLSAETWDLL RLPLEREQNG DSHHSGDWRG PSRDSLPLPV
460 470 480 490 500
RSRKYQEGPD AERRPREGSH SPLDSADVRV HVPRTSIPRA PSSDEECFFD
510 520 530 540 550
LLTKFQSSRM DDQRCPLDDG QAGAAEATAA PTLEDRIAQP SMTASPQTEE
560 570 580 590 600
FFDLIASSQS RRLDDQRASV GSLPGLRITH SNAGHLRGHG EPQEPGDDFF
610 620 630 640 650
NMLIKYQSSR IDDQRCPPPD VLPRGPTMPD EDFFSLIQRV QAKRMDEQRV
660 670
DLAGGPEQGA GGPPEPQQQC QPGAS
Length:675
Mass (Da):74,510
Last modified:April 20, 2010 - v2
Checksum:i27EB86667EEC1951
GO
Isoform 2 (identifier: Q86YR5-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-509: Missing.

Note: Minor isoform.
Show »
Length:166
Mass (Da):18,024
Checksum:iB0009DE5201BD2BD
GO
Isoform 3 (identifier: Q86YR5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     404-457: ARPKRTQRLS...LPVRSRKYQE → EFQGCGGVLL...FFLASGTAQA
     458-675: Missing.

Note: No experimental confirmation available.
Show »
Length:457
Mass (Da):49,685
Checksum:i4493B11B5D9986DC
GO
Isoform 4 (identifier: Q86YR5-4) [UniParc]FASTAAdd to basket
Also known as: FL

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: Major isoform.
Show »
Length:652
Mass (Da):72,136
Checksum:iFF690E5EC1999611
GO

Sequence cautioni

The sequence AAH09979 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH17353 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB55951 differs from that shown. Reason: Frameshift at position 657.Curated
The sequence CAI19339 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0209371 – 509Missing in isoform 2. 1 PublicationAdd BLAST509
Alternative sequenceiVSP_0390281 – 23Missing in isoform 4. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_020938404 – 457ARPKR…RKYQE → EFQGCGGVLLPTGTDRIRSC GGVGSRPGQHGGGGSRQKMA PTSQFFLASGTAQA in isoform 3. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_020939458 – 675Missing in isoform 3. 1 PublicationAdd BLAST218

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY173053 mRNA. Translation: AAO17260.1.
BX649589 Genomic DNA. Translation: CAI19338.1.
BX649589 Genomic DNA. Translation: CAI19339.1. Sequence problems.
BX649589 Genomic DNA. Translation: CAM27654.1.
CH471090 Genomic DNA. Translation: EAW88216.1.
BC009979 mRNA. Translation: AAH09979.1. Different initiation.
BC017353 mRNA. Translation: AAH17353.1. Different initiation.
BC048343 mRNA. Translation: AAH48343.1.
AL117478 mRNA. Translation: CAB55951.1. Frameshift.
CCDSiCCDS48055.1. [Q86YR5-1]
CCDS48056.1. [Q86YR5-2]
CCDS6996.2. [Q86YR5-3]
PIRiT17261.
RefSeqiNP_001139110.2. NM_001145638.2.
NP_001139111.1. NM_001145639.1. [Q86YR5-2]
NP_001186932.1. NM_001200003.1. [Q86YR5-2]
NP_056412.5. NM_015597.5.
XP_016870088.1. XM_017014599.1. [Q86YR5-2]
UniGeneiHs.239370.

Genome annotation databases

EnsembliENST00000291775; ENSP00000291775; ENSG00000160360. [Q86YR5-2]
ENST00000392944; ENSP00000376673; ENSG00000160360. [Q86YR5-2]
ENST00000429455; ENSP00000390705; ENSG00000160360. [Q86YR5-2]
GeneIDi26086.
KEGGihsa:26086.
UCSCiuc004che.3. human. [Q86YR5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY173053 mRNA. Translation: AAO17260.1.
BX649589 Genomic DNA. Translation: CAI19338.1.
BX649589 Genomic DNA. Translation: CAI19339.1. Sequence problems.
BX649589 Genomic DNA. Translation: CAM27654.1.
CH471090 Genomic DNA. Translation: EAW88216.1.
BC009979 mRNA. Translation: AAH09979.1. Different initiation.
BC017353 mRNA. Translation: AAH17353.1. Different initiation.
BC048343 mRNA. Translation: AAH48343.1.
AL117478 mRNA. Translation: CAB55951.1. Frameshift.
CCDSiCCDS48055.1. [Q86YR5-1]
CCDS48056.1. [Q86YR5-2]
CCDS6996.2. [Q86YR5-3]
PIRiT17261.
RefSeqiNP_001139110.2. NM_001145638.2.
NP_001139111.1. NM_001145639.1. [Q86YR5-2]
NP_001186932.1. NM_001200003.1. [Q86YR5-2]
NP_056412.5. NM_015597.5.
XP_016870088.1. XM_017014599.1. [Q86YR5-2]
UniGeneiHs.239370.

3D structure databases

ProteinModelPortaliQ86YR5.
SMRiQ86YR5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117539. 26 interactors.
IntActiQ86YR5. 7 interactors.
MINTiMINT-1453185.
STRINGi9606.ENSP00000392828.

PTM databases

iPTMnetiQ86YR5.
PhosphoSitePlusiQ86YR5.

Polymorphism and mutation databases

DMDMi294862435.

Proteomic databases

EPDiQ86YR5.
MaxQBiQ86YR5.
PaxDbiQ86YR5.
PeptideAtlasiQ86YR5.
PRIDEiQ86YR5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291775; ENSP00000291775; ENSG00000160360. [Q86YR5-2]
ENST00000392944; ENSP00000376673; ENSG00000160360. [Q86YR5-2]
ENST00000429455; ENSP00000390705; ENSG00000160360. [Q86YR5-2]
GeneIDi26086.
KEGGihsa:26086.
UCSCiuc004che.3. human. [Q86YR5-1]

Organism-specific databases

CTDi26086.
DisGeNETi26086.
GeneCardsiGPSM1.
HGNCiHGNC:17858. GPSM1.
HPAiHPA042199.
MIMi609491. gene.
neXtProtiNX_Q86YR5.
OpenTargetsiENSG00000160360.
PharmGKBiPA134986171.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1130. Eukaryota.
ENOG410XP6N. LUCA.
GeneTreeiENSGT00530000063126.
HOGENOMiHOG000231543.
HOVERGENiHBG051823.
InParanoidiQ86YR5.
KOiK15839.
PhylomeDBiQ86YR5.
TreeFamiTF328344.

Enzyme and pathway databases

ReactomeiR-HSA-418594. G alpha (i) signalling events.

Miscellaneous databases

ChiTaRSiGPSM1. human.
GeneWikiiGPSM1.
GenomeRNAii26086.
PROiQ86YR5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160360.
CleanExiHS_GPSM1.
ExpressionAtlasiQ86YR5. baseline and differential.
GenevisibleiQ86YR5. HS.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024804. G_prot_signal_mod_1.
IPR003109. GoLoco_motif.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10098:SF36. PTHR10098:SF36. 1 hit.
PfamiPF02188. GoLoco. 4 hits.
[Graphical view]
SMARTiSM00390. GoLoco. 4 hits.
SM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50877. GOLOCO. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPSM1_HUMAN
AccessioniPrimary (citable) accession number: Q86YR5
Secondary accession number(s): A9Z1X4
, B1B0W3, Q86SR5, Q969T1, Q9UFS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.