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Protein

Transcriptional repressor p66-alpha

Gene

GATAD2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri411 – 464GATA-typePROSITE-ProRule annotationAdd BLAST54

GO - Molecular functioni

GO - Biological processi

  • DNA methylation Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3214815. HDACs deacetylate histones.
R-HSA-427389. ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression.
R-HSA-6804758. Regulation of TP53 Activity through Acetylation.
R-HSA-73762. RNA Polymerase I Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor p66-alpha
Short name:
Hp66alpha
Alternative name(s):
GATA zinc finger domain-containing protein 2A
Gene namesi
Name:GATAD2A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:29989. GATAD2A.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • NuRD complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi149K → R: Disruption of MBD2-binding, loss of enhancement of MBD2-mediated repression and loss of speckled nuclear localization. 1 Publication1

Organism-specific databases

DisGeNETi54815.
OpenTargetsiENSG00000167491.
PharmGKBiPA142671746.

Polymorphism and mutation databases

BioMutaiGATAD2A.
DMDMi50401012.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000835001 – 633Transcriptional repressor p66-alphaAdd BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphothreonineCombined sources1
Modified residuei49PhosphothreonineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei113PhosphoserineBy similarity1
Modified residuei114PhosphoserineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei189PhosphothreonineCombined sources1
Modified residuei225Omega-N-methylarginineBy similarity1
Modified residuei249Omega-N-methylarginineCombined sources1
Modified residuei258Omega-N-methylarginineCombined sources1
Modified residuei273Omega-N-methylarginineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei285Omega-N-methylarginineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Cross-linki487Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei512PhosphoserineCombined sources1
Modified residuei539Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei539Omega-N-methylarginine; alternateCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei548PhosphoserineCombined sources1
Cross-linki550Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei556PhosphoserineCombined sources1
Modified residuei598PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86YP4.
MaxQBiQ86YP4.
PaxDbiQ86YP4.
PeptideAtlasiQ86YP4.
PRIDEiQ86YP4.

PTM databases

iPTMnetiQ86YP4.
PhosphoSitePlusiQ86YP4.

Miscellaneous databases

PMAP-CutDBQ86YP4.

Expressioni

Tissue specificityi

Ubiquitous, both in fetal and adult tissues.1 Publication

Gene expression databases

BgeeiENSG00000167491.
CleanExiHS_GATAD2A.
ExpressionAtlasiQ86YP4. baseline and differential.
GenevisibleiQ86YP4. HS.

Organism-specific databases

HPAiHPA006759.
HPA024373.

Interactioni

Subunit structurei

Binds MBD2 and MBD3. Interaction with MBD2 is required for the enhancement of MBD2-mediated repression and for targeting to the chromatin. Component of the MeCP1 histone deacetylase complex. Interacts with histone tails, including that of histones H2A, H2B, H3 and H4. This interaction is reduced by histone acetylation.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT40Q6A1623EBI-10261080,EBI-10171697

GO - Molecular functioni

  • protein binding, bridging Source: UniProtKB

Protein-protein interaction databases

BioGridi120172. 49 interactors.
DIPiDIP-36053N.
IntActiQ86YP4. 36 interactors.
MINTiMINT-1387810.
STRINGi9606.ENSP00000351552.

Structurei

Secondary structure

1633
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi138 – 168Combined sources31

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L2LNMR-A137-178[»]
ProteinModelPortaliQ86YP4.
SMRiQ86YP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni144 – 178CR1; MBD2-bindingAdd BLAST35
Regioni340 – 480CR2; histone tail-bindingAdd BLAST141

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili139 – 174Sequence analysisAdd BLAST36

Domaini

Both CR1 and CR2 regions are required for speckled nuclear localization.

Sequence similaritiesi

Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri411 – 464GATA-typePROSITE-ProRule annotationAdd BLAST54

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG3740. Eukaryota.
ENOG410XRVM. LUCA.
GeneTreeiENSGT00390000004097.
HOGENOMiHOG000074070.
HOVERGENiHBG053401.
InParanoidiQ86YP4.
OMAiLVNIPQP.
PhylomeDBiQ86YP4.
TreeFamiTF321369.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR032346. P66_CC.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
PF16563. P66_CC. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YP4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEEACRTRS QKRALERDPT EDDVESKKIK MERGLLASDL NTDGDMRVTP
60 70 80 90 100
EPGAGPTQGL LRATEATAMA MGRGEGLVGD GPVDMRTSHS DMKSERRPPS
110 120 130 140 150
PDVIVLSDNE QPSSPRVNGL TTVALKETST EALMKSSPEE RERMIKQLKE
160 170 180 190 200
ELRLEEAKLV LLKKLRQSQI QKEATAQKPT GSVGSTVTTP PPLVRGTQNI
210 220 230 240 250
PAGKPSLQTS SARMPGSVIP PPLVRGGQQA SSKLGPQASS QVVMPPLVRG
260 270 280 290 300
AQQIHSIRQH SSTGPPPLLL APRASVPSVQ IQGQRIIQQG LIRVANVPNT
310 320 330 340 350
SLLVNIPQPT PASLKGTTAT SAQANSTPTS VASVVTSAES PASRQAAAKL
360 370 380 390 400
ALRKQLEKTL LEIPPPKPPA PEMNFLPSAA NNEFIYLVGL EEVVQNLLET
410 420 430 440 450
QGRMSAATVL SREPYMCAQC KTDFTCRWRE EKSGAIMCEN CMTTNQKKAL
460 470 480 490 500
KVEHTSRLKA AFVKALQQEQ EIEQRLLQQG TAPAQAKAEP TAAPHPVLKQ
510 520 530 540 550
VIKPRRKLAF RSGEARDWSN GAVLQASSQL SRGSATTPRG VLHTFSPSPK
560 570 580 590 600
LQNSASATAL VSRTGRHSER TVSAGKGSAT SNWKKTPLST GGTLAFVSPS
610 620 630
LAVHKSSSAV DRQREYLLDM IPPRSIPQSA TWK
Length:633
Mass (Da):68,063
Last modified:June 1, 2003 - v1
Checksum:i64F0066B4BEFCB39
GO
Isoform 2 (identifier: Q86YP4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     500-524: Missing.

Note: No experimental confirmation available.
Show »
Length:608
Mass (Da):65,225
Checksum:i751DB0FC42DA3BC8
GO
Isoform 3 (identifier: Q86YP4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     401-401: Q → QA

Note: No experimental confirmation available.
Show »
Length:634
Mass (Da):68,134
Checksum:i4C5D2B9CD5FE719E
GO

Sequence cautioni

The sequence AAH11684 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA90939 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti432K → E in BAA90939 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05930817R → Q.Corresponds to variant rs10426883dbSNPEnsembl.1
Natural variantiVAR_059309296N → S.Corresponds to variant rs2288851dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053410401Q → QA in isoform 3. 1 Publication1
Alternative sequenceiVSP_010929500 – 524Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186731 mRNA. Translation: AAO31797.1.
AC003030 Genomic DNA. No translation available.
AC011448 Genomic DNA. No translation available.
AC092067 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84819.1.
BC011684 mRNA. Translation: AAH11684.1. Different initiation.
BC012902 mRNA. Translation: AAH12902.2.
AK000092 mRNA. Translation: BAA90939.1. Different initiation.
AL390164 mRNA. Translation: CAB99095.1.
CCDSiCCDS12402.2. [Q86YP4-1]
CCDS77270.1. [Q86YP4-3]
PIRiT51878.
RefSeqiNP_001287875.1. NM_001300946.1. [Q86YP4-3]
NP_060130.3. NM_017660.3. [Q86YP4-1]
XP_011526407.1. XM_011528105.1. [Q86YP4-3]
UniGeneiHs.118964.

Genome annotation databases

EnsembliENST00000358713; ENSP00000351552; ENSG00000167491. [Q86YP4-1]
ENST00000360315; ENSP00000353463; ENSG00000167491. [Q86YP4-1]
ENST00000404158; ENSP00000384899; ENSG00000167491. [Q86YP4-3]
GeneIDi54815.
KEGGihsa:54815.
UCSCiuc010xqt.3. human. [Q86YP4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY186731 mRNA. Translation: AAO31797.1.
AC003030 Genomic DNA. No translation available.
AC011448 Genomic DNA. No translation available.
AC092067 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84819.1.
BC011684 mRNA. Translation: AAH11684.1. Different initiation.
BC012902 mRNA. Translation: AAH12902.2.
AK000092 mRNA. Translation: BAA90939.1. Different initiation.
AL390164 mRNA. Translation: CAB99095.1.
CCDSiCCDS12402.2. [Q86YP4-1]
CCDS77270.1. [Q86YP4-3]
PIRiT51878.
RefSeqiNP_001287875.1. NM_001300946.1. [Q86YP4-3]
NP_060130.3. NM_017660.3. [Q86YP4-1]
XP_011526407.1. XM_011528105.1. [Q86YP4-3]
UniGeneiHs.118964.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L2LNMR-A137-178[»]
ProteinModelPortaliQ86YP4.
SMRiQ86YP4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120172. 49 interactors.
DIPiDIP-36053N.
IntActiQ86YP4. 36 interactors.
MINTiMINT-1387810.
STRINGi9606.ENSP00000351552.

PTM databases

iPTMnetiQ86YP4.
PhosphoSitePlusiQ86YP4.

Polymorphism and mutation databases

BioMutaiGATAD2A.
DMDMi50401012.

Proteomic databases

EPDiQ86YP4.
MaxQBiQ86YP4.
PaxDbiQ86YP4.
PeptideAtlasiQ86YP4.
PRIDEiQ86YP4.

Protocols and materials databases

DNASUi54815.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358713; ENSP00000351552; ENSG00000167491. [Q86YP4-1]
ENST00000360315; ENSP00000353463; ENSG00000167491. [Q86YP4-1]
ENST00000404158; ENSP00000384899; ENSG00000167491. [Q86YP4-3]
GeneIDi54815.
KEGGihsa:54815.
UCSCiuc010xqt.3. human. [Q86YP4-1]

Organism-specific databases

CTDi54815.
DisGeNETi54815.
GeneCardsiGATAD2A.
H-InvDBHIX0014940.
HGNCiHGNC:29989. GATAD2A.
HPAiHPA006759.
HPA024373.
MIMi614997. gene.
neXtProtiNX_Q86YP4.
OpenTargetsiENSG00000167491.
PharmGKBiPA142671746.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3740. Eukaryota.
ENOG410XRVM. LUCA.
GeneTreeiENSGT00390000004097.
HOGENOMiHOG000074070.
HOVERGENiHBG053401.
InParanoidiQ86YP4.
OMAiLVNIPQP.
PhylomeDBiQ86YP4.
TreeFamiTF321369.

Enzyme and pathway databases

ReactomeiR-HSA-3214815. HDACs deacetylate histones.
R-HSA-427389. ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression.
R-HSA-6804758. Regulation of TP53 Activity through Acetylation.
R-HSA-73762. RNA Polymerase I Transcription Initiation.

Miscellaneous databases

ChiTaRSiGATAD2A. human.
GenomeRNAii54815.
PMAP-CutDBQ86YP4.
PROiQ86YP4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167491.
CleanExiHS_GATAD2A.
ExpressionAtlasiQ86YP4. baseline and differential.
GenevisibleiQ86YP4. HS.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiIPR032346. P66_CC.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00320. GATA. 1 hit.
PF16563. P66_CC. 1 hit.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 1 hit.
PS50114. GATA_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP66A_HUMAN
AccessioniPrimary (citable) accession number: Q86YP4
Secondary accession number(s): B5MC40
, Q7L3J2, Q96F28, Q9NPU2, Q9NXS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.