Q86YM7 (HOME1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homer protein homolog 1
Short name=Homer-1

Gene names

Name:	HOMER1
Synonyms:	SYN47

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
Subunit structure	Interacts with IFT57 By similarity. Interacts with OPHN1 By similarity. Isoform 1 encodes a coiled-coil structure that mediates homo- and heteromultimerization. Ref.8
Subcellular location	Cytoplasm By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Cell junction › synapse By similarity. Note: Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum By similarity.
Domain	The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Sequence similarities	Belongs to the Homer family. Contains 1 WH1 domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of phospholipase C activity by G-protein coupled glutamate receptor signaling pathway Traceable author statement. Source: ProtInc synaptic transmission Traceable author statement. Source: ProtInc
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement. Source: ProtInc postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86YM7-1)

Also known as: 1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86YM7-2)

Also known as: 1e;

The sequence of this isoform differs from the canonical sequence as follows:
     99-228: Missing.

Isoform 3 (identifier: Q86YM7-3)

Also known as: 1h;

The sequence of this isoform differs from the canonical sequence as follows:
     176-177: SS → RK
     178-354: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Homer protein homolog 1		PRO_0000191005
Regions
	Domain	1 – 110	110	WH1
	Coiled coil	181 – 352	172	Potential
Natural variations
	Alternative sequence	99 – 228	130	Missing in isoform 2.		VSP_009057
	Alternative sequence	176 – 177	2	SS → RK in isoform 3.		VSP_009058
	Alternative sequence	178 – 354	177	Missing in isoform 3.		VSP_009059

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (1b) [UniParc]. Last modified November 28, 2003. Version 2. Checksum: 8C68A56224C98033	FASTA	354	40,277
	10 20 30 40 50 60 MGEQPIFSTR AHVFQIDPNT KKNWVPTSKH AVTVSYFYDS TRNVYRIISL DGSKAIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVYGLGF SSEHHLSKFA EKFQEFKEAA RLAKEKSQEK 130 140 150 160 170 180 MELTSTPSQE SAGGDLQSPL TPESINGTDD ERTPDVTQNS EPRAEPTQNA LPFSHSSAIS 190 200 210 220 230 240 KHWEAELATL KGNNAKLTAA LLESTANVKQ WKQQLAAYQE EAERLHKRVT ELECVSSQAN 250 260 270 280 290 300 AVHTHKTELN QTIQELEETL KLKEEEIERL KQEIDNAREL QEQRDSLTQK LQEVEIRNKD 310 320 330 340 350 LEGQLSDLEQ RLEKSQNEQE AFRNNLKTLL EILDGKIFEL TELRDNLAKL LECS
	Isoform 2 (1e) [UniParc]. Checksum: F94F6A9AB2682629	FASTA	224	25,843
	10 20 30 40 50 60 MGEQPIFSTR AHVFQIDPNT KKNWVPTSKH AVTVSYFYDS TRNVYRIISL DGSKAIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVYGLGF SSEHHLSKVT ELECVSSQAN AVHTHKTELN 130 140 150 160 170 180 QTIQELEETL KLKEEEIERL KQEIDNAREL QEQRDSLTQK LQEVEIRNKD LEGQLSDLEQ 190 200 210 220 RLEKSQNEQE AFRNNLKTLL EILDGKIFEL TELRDNLAKL LECS
	Isoform 3 (1h) [UniParc]. Checksum: B1AE94C8E245CAEF	FASTA	177	19,861
	10 20 30 40 50 60 MGEQPIFSTR AHVFQIDPNT KKNWVPTSKH AVTVSYFYDS TRNVYRIISL DGSKAIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVYGLGF SSEHHLSKFA EKFQEFKEAA RLAKEKSQEK 130 140 150 160 170 MELTSTPSQE SAGGDLQSPL TPESINGTDD ERTPDVTQNS EPRAEPTQNA LPFSHRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F. Neuron 21:707-716(1998) [PubMed: 9808458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Frontal cortex.
[2]	"Cloning and molecular characterization of a human cDNA Syn47 showing strong homology to the cDNA of the rat protein Homer." Nickels A., Heckel D., Sahin U., Tuereci O., Koch B., Meese E., Pfreundschuh M., Montenarh M. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Synovial cell.
[3]	"Multiple splice isoforms of the HOMER1 gene." Klugmann M., Leichtlein C.B., Klaussner B.K., During M.J. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3). Tissue: Brain.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hippocampus.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreas.
[8]	"Homer 1b regulates the trafficking of group I metabotropic glutamate receptors." Roche K.W., Tu J.C., Petralia R.S., Xiao B., Wenthold R.J., Worley P.F. J. Biol. Chem. 274:25953-25957(1999) [PubMed: 10464340] [Abstract] Cited for: INTERACTION WITH GRM5.
[9]	"Homer: a link between neural activity and glutamate receptor function." Xiao B., Tu J.C., Worley P.F. Curr. Opin. Neurobiol. 10:370-374(2000) [PubMed: 10851183] [Abstract] Cited for: REVIEW.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093262 mRNA. Translation: AAC71026.1. Y17829 mRNA. Translation: CAA76877.1. AY189939 mRNA. Translation: AAO38999.1. AY189941 mRNA. Translation: AAO39001.1. BT009846 mRNA. Translation: AAP88848.1. AK312481 mRNA. Translation: BAG35385.1. CH471084 Genomic DNA. Translation: EAW95836.1. BC015502 mRNA. Translation: AAH15502.1.
IPI	IPI00003566. IPI00384726. IPI00394997.
RefSeq	NP_004263.1. NM_004272.3.
UniGene	Hs.591761.
3D structure databases
ProteinModelPortal	Q86YM7.
SMR	Q86YM7. Positions 1-143, 290-352.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86YM7. 5 interactions.
MINT	MINT-1481241.
STRING	Q86YM7.
PTM databases
PhosphoSite	Q86YM7.
Polymorphism databases
DMDM	38604765.
Proteomic databases
PRIDE	Q86YM7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334082; ENSP00000334382; ENSG00000152413.
GeneID	9456.
KEGG	hsa:9456.
NMPDR	fig|9606.3.peg.25452.
UCSC	uc003kfy.1. human. uc010jab.1. human. uc010jac.1. human.
Organism-specific databases
CTD	9456.
GeneCards	GC05M078705.
H-InvDB	HIX0004984.
HGNC	HGNC:17512. HOMER1.
HPA	CAB004437.
MIM	604798. gene.
neXtProt	NX_Q86YM7.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15581.
GeneTree	ENSGT00390000017850.
HOGENOM	HBG443677.
HOVERGEN	HBG051918.
InParanoid	Q86YM7.
OMA	TSTPSQX.
OrthoDB	EOG44QT1P.
PhylomeDB	Q86YM7.
Gene expression databases
ArrayExpress	Q86YM7.
Bgee	Q86YM7.
CleanEx	HS_HOMER1.
Genevestigator	Q86YM7.
GermOnline	ENSG00000152413. Homo sapiens.
Family and domain databases
InterPro	IPR000697. EVH1. IPR010356. Haemolysin_E. IPR011993. PH_type. [Graphical view]
Gene3D	G3DSA:1.20.1170.10. Haemolysin_E. 1 hit. G3DSA:2.30.29.30. PH_type. 1 hit.
KO	K15010.
Pfam	PF00568. WH1. 1 hit. [Graphical view]
SMART	SM00461. WH1. 1 hit. [Graphical view]
PROSITE	PS50229. WH1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	35434.
SOURCE	Search...

Entry information

Entry name

HOME1_HUMAN

Accession

Primary (citable) accession number: Q86YM7
Secondary accession number(s): B2R688, O96003, Q86YM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.