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Protein

Ankyrin repeat domain-containing protein 13B

Gene

ANKRD13B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.1 Publication

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein 13B
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:26363. ANKRD13B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672616.

Polymorphism and mutation databases

BioMutaiANKRD13B.
DMDMi269849747.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626Ankyrin repeat domain-containing protein 13BPRO_0000240643Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ86YJ7.
MaxQBiQ86YJ7.
PaxDbiQ86YJ7.
PRIDEiQ86YJ7.

PTM databases

iPTMnetiQ86YJ7.
PhosphoSiteiQ86YJ7.

Expressioni

Gene expression databases

BgeeiQ86YJ7.
CleanExiHS_ANKRD13B.
ExpressionAtlasiQ86YJ7. baseline and differential.
GenevisibleiQ86YJ7. HS.

Organism-specific databases

HPAiHPA051886.
HPA063748.

Interactioni

Subunit structurei

Interacts with EGFR (ubiquitinated); the interaction is direct and may regulate EGFR internalization.1 Publication

Protein-protein interaction databases

BioGridi125900. 9 interactions.
IntActiQ86YJ7. 5 interactions.
MINTiMINT-1421772.
STRINGi9606.ENSP00000378328.

Structurei

3D structure databases

ProteinModelPortaliQ86YJ7.
SMRiQ86YJ7. Positions 14-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati47 – 7630ANK 1Add
BLAST
Repeati80 – 10930ANK 2Add
BLAST
Domaini503 – 52220UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini585 – 60420UIM 2PROSITE-ProRule annotationAdd
BLAST
Domaini610 – 62617UIM 3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi445 – 47228Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 3 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0522. Eukaryota.
ENOG410XNMS. LUCA.
GeneTreeiENSGT00410000025381.
HOGENOMiHOG000230821.
HOVERGENiHBG059287.
InParanoidiQ86YJ7.
OMAiGRVFRSY.
OrthoDBiEOG76QFGX.
PhylomeDBiQ86YJ7.
TreeFamiTF314176.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR021832. ANKRD13.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12447. PTHR12447. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF11904. GPCR_chapero_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YJ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIPANASARK GPEGKYPLHY LVWHNRHREL EKEVRAGQVD IEQLDPRGRT
60 70 80 90 100
PLHLATTLGH LECARVLLAH GADVGRENRS GWTVLQEAVS TRDLELVQLV
110 120 130 140 150
LRYRDYQRVV KRLAGIPVLL EKLRKAQDFY VEMKWEFTSW VPLVSKICPS
160 170 180 190 200
DTYKVWKSGQ NLRVDTTLLG FDHMTWQRGN RSFVFRGQDT SAVVMEIDHD
210 220 230 240 250
RRVVYTETLA LAGQDRELLL AAAQPTEEQV LSRLTAPVVT TQLDTKNISF
260 270 280 290 300
ERNKTGILGW RSEKTEMVNG YEAKVYGASN VELITRTRTE HLSEQHKGKV
310 320 330 340 350
KGCKTPLQSF LGIAEQHGGP QNGTLITQTL SQANPTAITA EEYFNPNFEL
360 370 380 390 400
GNRDMGRPME LTTKTQKFKA KLWLCEEHPL SLCEQVAPII DLMAVSNALF
410 420 430 440 450
AKLRDFITLR LPPGFPVKIE IPIFHILNAR ITFGNLNGCD EPVPSVRGSP
460 470 480 490 500
SSETPSPGSD SSSVSSSSST TSCRGCEISP ALFEAPRGYS MMGGQREAAT
510 520 530 540 550
RDDDDDLLQF AIQQSLLEAG SEYDQVTIWE ALTNSKPGTH PMSYEGRRQD
560 570 580 590 600
RSAPPTPQRQ PAPPASVPSP RPSSGPGSGG HVFRSYDEQL RLAMELSAQE
610 620
QEERRRRARQ EEEELERILR LSLTEQ
Length:626
Mass (Da):70,205
Last modified:November 24, 2009 - v4
Checksum:i8807A784A21F395C
GO
Isoform 2 (identifier: Q86YJ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-132: Missing.

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):55,056
Checksum:i38961D1F8493EE0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261A → P in AAH32554 (PubMed:15489334).Curated
Sequence conflicti295 – 2951Q → H in AAH32554 (PubMed:15489334).Curated
Sequence conflicti304 – 3041K → E in BAC05148 (PubMed:14702039).Curated
Sequence conflicti332 – 3321Q → R in BAC05148 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 132132Missing in isoform 2. 1 PublicationVSP_019404Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK097716 mRNA. Translation: BAC05148.1.
AC104564 Genomic DNA. No translation available.
BC032554 mRNA. Translation: AAH32554.3.
CCDSiCCDS11251.1. [Q86YJ7-1]
RefSeqiNP_689558.4. NM_152345.4. [Q86YJ7-1]
UniGeneiHs.662164.

Genome annotation databases

EnsembliENST00000394859; ENSP00000378328; ENSG00000198720. [Q86YJ7-1]
ENST00000488766; ENSP00000431958; ENSG00000198720. [Q86YJ7-1]
ENST00000614878; ENSP00000481530; ENSG00000198720. [Q86YJ7-1]
GeneIDi124930.
KEGGihsa:124930.
UCSCiuc002hei.4. human. [Q86YJ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK097716 mRNA. Translation: BAC05148.1.
AC104564 Genomic DNA. No translation available.
BC032554 mRNA. Translation: AAH32554.3.
CCDSiCCDS11251.1. [Q86YJ7-1]
RefSeqiNP_689558.4. NM_152345.4. [Q86YJ7-1]
UniGeneiHs.662164.

3D structure databases

ProteinModelPortaliQ86YJ7.
SMRiQ86YJ7. Positions 14-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125900. 9 interactions.
IntActiQ86YJ7. 5 interactions.
MINTiMINT-1421772.
STRINGi9606.ENSP00000378328.

PTM databases

iPTMnetiQ86YJ7.
PhosphoSiteiQ86YJ7.

Polymorphism and mutation databases

BioMutaiANKRD13B.
DMDMi269849747.

Proteomic databases

EPDiQ86YJ7.
MaxQBiQ86YJ7.
PaxDbiQ86YJ7.
PRIDEiQ86YJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394859; ENSP00000378328; ENSG00000198720. [Q86YJ7-1]
ENST00000488766; ENSP00000431958; ENSG00000198720. [Q86YJ7-1]
ENST00000614878; ENSP00000481530; ENSG00000198720. [Q86YJ7-1]
GeneIDi124930.
KEGGihsa:124930.
UCSCiuc002hei.4. human. [Q86YJ7-1]

Organism-specific databases

CTDi124930.
GeneCardsiANKRD13B.
HGNCiHGNC:26363. ANKRD13B.
HPAiHPA051886.
HPA063748.
MIMi615124. gene.
neXtProtiNX_Q86YJ7.
PharmGKBiPA142672616.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0522. Eukaryota.
ENOG410XNMS. LUCA.
GeneTreeiENSGT00410000025381.
HOGENOMiHOG000230821.
HOVERGENiHBG059287.
InParanoidiQ86YJ7.
OMAiGRVFRSY.
OrthoDBiEOG76QFGX.
PhylomeDBiQ86YJ7.
TreeFamiTF314176.

Miscellaneous databases

GenomeRNAii124930.
PROiQ86YJ7.
SOURCEiSearch...

Gene expression databases

BgeeiQ86YJ7.
CleanExiHS_ANKRD13B.
ExpressionAtlasiQ86YJ7. baseline and differential.
GenevisibleiQ86YJ7. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR021832. ANKRD13.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003903. UIM_dom.
[Graphical view]
PANTHERiPTHR12447. PTHR12447. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF11904. GPCR_chapero_1. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  4. "The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane."
    Tanno H., Yamaguchi T., Goto E., Ishido S., Komada M.
    Mol. Biol. Cell 23:1343-1353(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EGFR, UBIQUITIN-BINDING, SUBCELLULAR LOCATION.
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAN13B_HUMAN
AccessioniPrimary (citable) accession number: Q86YJ7
Secondary accession number(s): Q8N7S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 24, 2009
Last modified: June 8, 2016
This is version 104 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.