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Q86YJ6 (THNS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine synthase-like 2

Short name=TSH2
EC=4.2.3.-
Alternative name(s):
Secreted osteoclastogenic factor of activated T-cells
Short name=SOFAT
Gene names
Name:THNSL2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Isoform 1:Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate By similarity. Ref.1

Isoform SOLAT:Potent inducer of osteoblastic production of IL6. May act to exacerbate inflammation and/or bone turnover under inflammatory conditions. Ref.1

Cofactor

Pyridoxal phosphate By similarity.

Subcellular location

Isoform SOLAT: Secreted. Note: Secreted by activated T-cells via a calcineurin-independent pathway. Ref.1

Sequence similarities

Belongs to the threonine synthase family.

Sequence caution

The sequence AAX88906.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAA91904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86YJ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86YJ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGG
Note: No experimental confirmation available.
Isoform 3 (identifier: Q86YJ6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     360-484: LSEAVTSVSV...WRSHALNTSQ → HSPVLPRPCLCSQVPGSCPGCWPDP
Note: No experimental confirmation available.
Isoform 4 (identifier: Q86YJ6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     214-274: Missing.
Note: No experimental confirmation available.
Isoform SOLAT (identifier: Q86YJ6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGD

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Threonine synthase-like 2
PRO_0000306407

Amino acid modifications

Modified residue1131N6-(pyridoxal phosphate)lysine By similarity

Natural variations

Alternative sequence1 – 158158Missing in isoform SOLAT.
VSP_041659
Alternative sequence214 – 27461Missing in isoform 4.
VSP_028468
Alternative sequence360 – 484125LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGG in isoform 2.
VSP_028469
Alternative sequence360 – 484125LSEAV…LNTSQ → HSPVLPRPCLCSQVPGSCPG CWPDP in isoform 3.
VSP_028470
Alternative sequence360 – 484125LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGD in isoform SOLAT.
VSP_041660
Natural variant411G → E. Ref.2 Ref.4
Corresponds to variant rs4129190 [ dbSNP | Ensembl ].
VAR_054635
Natural variant1081V → I.
Corresponds to variant rs35541720 [ dbSNP | Ensembl ].
VAR_054636
Natural variant2041T → N.
Corresponds to variant rs34136143 [ dbSNP | Ensembl ].
VAR_054637
Natural variant3241R → G. Ref.4
Corresponds to variant rs17855905 [ dbSNP | Ensembl ].
VAR_054638

Experimental info

Sequence conflict631I → V in BAA91904. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 30, 2010. Version 3.
Checksum: F177B2B7DE6E8752

FASTA48454,116
        10         20         30         40         50         60 
MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS YPGLVKELCA 

        70         80         90        100        110        120 
LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN VLELWHGVTY AFKDLSLSCT 

       130        140        150        160        170        180 
TQFLQYFLEK REKHVTVVVG TSGDTGSAAI ESVQGAKNMD IIVLLPKGHC TKIQELQMTT 

       190        200        210        220        230        240 
VLKQNVHVFG VEGNSDELDE PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF 

       250        260        270        280        290        300 
QCTPSLDTHP LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF 

       310        320        330        340        350        360 
SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV NLPKELHSKL 

       370        380        390        400        410        420 
SEAVTSVSVS DEAITQTMGR CWDENQYLLC PHSAVAVNYH YQQIDRQQPS TPRCCLAPAS 

       430        440        450        460        470        480 
AAKFPEAVLA AGLTPETPAE IVALEHKETR CTLMRRGDNW MLMLRDTIED LSRQWRSHAL 


NTSQ 

« Hide

Isoform 2 [UniParc].

Checksum: 5A2B03980DD1B3ED
Show »

FASTA40545,037
Isoform 3 [UniParc].

Checksum: BE36DE86123D5733
Show »

FASTA38442,718
Isoform 4 [UniParc].

Checksum: 14A1A6D8308BC93F
Show »

FASTA42347,428
Isoform SOLAT [UniParc].

Checksum: 0124469439FCFD9D
Show »

FASTA24727,414

References

« Hide 'large scale' references
[1]"A novel T cell cytokine, secreted osteoclastogenic factor of activated T cells, induces osteoclast formation in a RANKL-independent manner."
Rifas L., Weitzmann M.N.
Arthritis Rheum. 60:3324-3335(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOLAT), FUNCTION, SUBCELLULAR LOCATION.
Tissue: Peripheral blood T-cell.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-484 (ISOFORM 4), VARIANT GLU-41.
Tissue: Ovarian carcinoma and Tongue.
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-484 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 140-431 (ISOFORM 3), VARIANTS GLU-41 AND GLY-324.
Tissue: Brain, Colon and Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HM185274 mRNA. Translation: ADL14696.1.
AK001778 mRNA. Translation: BAA91904.1. Different initiation.
AK095303 mRNA. Translation: BAG53023.1.
AC092836 Genomic DNA. Translation: AAX88906.1. Sequence problems.
BC035315 mRNA. Translation: AAH35315.1.
BC047758 mRNA. Translation: AAH47758.2.
BC064423 mRNA. Translation: AAH64423.1.
CCDSCCDS2002.2. [Q86YJ6-1]
CCDS58718.1. [Q86YJ6-2]
RefSeqNP_001231605.1. NM_001244676.1. [Q86YJ6-2]
NP_060741.3. NM_018271.4. [Q86YJ6-1]
XP_005264457.1. XM_005264400.2. [Q86YJ6-1]
XP_005264458.1. XM_005264401.2. [Q86YJ6-1]
XP_005264460.1. XM_005264403.2. [Q86YJ6-3]
XP_006712106.1. XM_006712043.1. [Q86YJ6-2]
UniGeneHs.739080.
Hs.740434.

3D structure databases

ProteinModelPortalQ86YJ6.
SMRQ86YJ6. Positions 3-428.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120549. 1 interaction.
IntActQ86YJ6. 2 interactions.
MINTMINT-1391634.

Polymorphism databases

DMDM313104279.

Proteomic databases

MaxQBQ86YJ6.
PaxDbQ86YJ6.
PRIDEQ86YJ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000324166; ENSP00000327323; ENSG00000144115. [Q86YJ6-1]
ENST00000343544; ENSP00000339563; ENSG00000144115. [Q86YJ6-2]
ENST00000358591; ENSP00000351402; ENSG00000144115. [Q86YJ6-1]
ENST00000377254; ENSP00000366464; ENSG00000144115. [Q86YJ6-3]
ENST00000402102; ENSP00000384475; ENSG00000144115. [Q86YJ6-2]
GeneID55258.
KEGGhsa:55258.
UCSCuc002ssw.4. human. [Q86YJ6-2]
uc002ssy.4. human. [Q86YJ6-1]

Organism-specific databases

CTD55258.
GeneCardsGC02P088469.
H-InvDBHIX0002247.
HGNCHGNC:25602. THNSL2.
HPAHPA035395.
MIM611261. gene.
neXtProtNX_Q86YJ6.
PharmGKBPA162405692.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0498.
HOVERGENHBG108568.
InParanoidQ86YJ6.
OMAWRSHALN.
OrthoDBEOG7QZG93.
PhylomeDBQ86YJ6.
TreeFamTF329641.

Enzyme and pathway databases

SignaLinkQ86YJ6.

Gene expression databases

ArrayExpressQ86YJ6.
BgeeQ86YJ6.
CleanExHS_THNSL2.
GenevestigatorQ86YJ6.

Family and domain databases

Gene3D3.90.1380.10. 1 hit.
InterProIPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
IPR027457. TSH2.
[Graphical view]
PANTHERPTHR10314:SF89. PTHR10314:SF89. 1 hit.
PfamPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMSSF53686. SSF53686. 1 hit.
TIGRFAMsTIGR00260. thrC. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi55258.
NextBio59344.
PROQ86YJ6.
SOURCESearch...

Entry information

Entry nameTHNS2_HUMAN
AccessionPrimary (citable) accession number: Q86YJ6
Secondary accession number(s): B3KTB1 expand/collapse secondary AC list , B5MDX8, B7WPF8, D9ZZB8, Q6P2M7, Q6PI27, Q9NV54
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: November 30, 2010
Last modified: July 9, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM