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Q86YJ6

- THNS2_HUMAN

UniProt

Q86YJ6 - THNS2_HUMAN

Protein

Threonine synthase-like 2

Gene

THNSL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 3 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Isoform 1: Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate By similarity.By similarity
    Isoform SOLAT: Potent inducer of osteoblastic production of IL6. May act to exacerbate inflammation and/or bone turnover under inflammatory conditions.1 Publication

    Cofactori

    Pyridoxal phosphate.By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. pyridoxal phosphate binding Source: BHF-UCL
    3. serine binding Source: BHF-UCL

    GO - Biological processi

    1. 2-oxobutyrate biosynthetic process Source: BHF-UCL
    2. dephosphorylation Source: BHF-UCL
    3. serine family amino acid catabolic process Source: BHF-UCL

    Keywords - Molecular functioni

    Cytokine, Lyase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    SignaLinkiQ86YJ6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine synthase-like 2 (EC:4.2.3.-)
    Short name:
    TSH2
    Alternative name(s):
    Secreted osteoclastogenic factor of activated T-cells
    Short name:
    SOFAT
    Gene namesi
    Name:THNSL2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:25602. THNSL2.

    Subcellular locationi

    Isoform SOLAT : Secreted
    Note: Secreted by activated T-cells via a calcineurin-independent pathway.

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162405692.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484Threonine synthase-like 2PRO_0000306407Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei113 – 1131N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiQ86YJ6.
    PaxDbiQ86YJ6.
    PRIDEiQ86YJ6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ86YJ6.
    BgeeiQ86YJ6.
    CleanExiHS_THNSL2.
    GenevestigatoriQ86YJ6.

    Organism-specific databases

    HPAiHPA035395.

    Interactioni

    Protein-protein interaction databases

    BioGridi120549. 1 interaction.
    IntActiQ86YJ6. 2 interactions.
    MINTiMINT-1391634.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86YJ6.
    SMRiQ86YJ6. Positions 3-428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the threonine synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0498.
    HOVERGENiHBG108568.
    InParanoidiQ86YJ6.
    OMAiWRSHALN.
    OrthoDBiEOG7QZG93.
    PhylomeDBiQ86YJ6.
    TreeFamiTF329641.

    Family and domain databases

    Gene3Di3.90.1380.10. 1 hit.
    InterProiIPR029144. Thr_synth_N.
    IPR004450. Thr_synthase_like.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    IPR027457. TSH2.
    [Graphical view]
    PANTHERiPTHR10314:SF89. PTHR10314:SF89. 1 hit.
    PfamiPF00291. PALP. 1 hit.
    PF14821. Thr_synth_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53686. SSF53686. 1 hit.
    TIGRFAMsiTIGR00260. thrC. 1 hit.

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86YJ6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS    50
    YPGLVKELCA LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN 100
    VLELWHGVTY AFKDLSLSCT TQFLQYFLEK REKHVTVVVG TSGDTGSAAI 150
    ESVQGAKNMD IIVLLPKGHC TKIQELQMTT VLKQNVHVFG VEGNSDELDE 200
    PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF QCTPSLDTHP 250
    LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF 300
    SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV 350
    NLPKELHSKL SEAVTSVSVS DEAITQTMGR CWDENQYLLC PHSAVAVNYH 400
    YQQIDRQQPS TPRCCLAPAS AAKFPEAVLA AGLTPETPAE IVALEHKETR 450
    CTLMRRGDNW MLMLRDTIED LSRQWRSHAL NTSQ 484
    Length:484
    Mass (Da):54,116
    Last modified:November 30, 2010 - v3
    Checksum:iF177B2B7DE6E8752
    GO
    Isoform 2 (identifier: Q86YJ6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGG

    Note: No experimental confirmation available.

    Show »
    Length:405
    Mass (Da):45,037
    Checksum:i5A2B03980DD1B3ED
    GO
    Isoform 3 (identifier: Q86YJ6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         360-484: LSEAVTSVSV...WRSHALNTSQ → HSPVLPRPCLCSQVPGSCPGCWPDP

    Note: No experimental confirmation available.

    Show »
    Length:384
    Mass (Da):42,718
    Checksum:iBE36DE86123D5733
    GO
    Isoform 4 (identifier: Q86YJ6-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         214-274: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:423
    Mass (Da):47,428
    Checksum:i14A1A6D8308BC93F
    GO
    Isoform SOLAT (identifier: Q86YJ6-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-158: Missing.
         360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGD

    Show »
    Length:247
    Mass (Da):27,414
    Checksum:i0124469439FCFD9D
    GO

    Sequence cautioni

    The sequence BAA91904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAX88906.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 631I → V in BAA91904. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti41 – 411G → E.2 Publications
    Corresponds to variant rs4129190 [ dbSNP | Ensembl ].
    VAR_054635
    Natural varianti108 – 1081V → I.
    Corresponds to variant rs35541720 [ dbSNP | Ensembl ].
    VAR_054636
    Natural varianti204 – 2041T → N.
    Corresponds to variant rs34136143 [ dbSNP | Ensembl ].
    VAR_054637
    Natural varianti324 – 3241R → G.1 Publication
    Corresponds to variant rs17855905 [ dbSNP | Ensembl ].
    VAR_054638

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 158158Missing in isoform SOLAT. 1 PublicationVSP_041659Add
    BLAST
    Alternative sequencei214 – 27461Missing in isoform 4. 1 PublicationVSP_028468Add
    BLAST
    Alternative sequencei360 – 484125LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGG in isoform 2. 1 PublicationVSP_028469Add
    BLAST
    Alternative sequencei360 – 484125LSEAV…LNTSQ → HSPVLPRPCLCSQVPGSCPG CWPDP in isoform 3. 1 PublicationVSP_028470Add
    BLAST
    Alternative sequencei360 – 484125LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGD in isoform SOLAT. 1 PublicationVSP_041660Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HM185274 mRNA. Translation: ADL14696.1.
    AK001778 mRNA. Translation: BAA91904.1. Different initiation.
    AK095303 mRNA. Translation: BAG53023.1.
    AC092836 Genomic DNA. Translation: AAX88906.1. Sequence problems.
    BC035315 mRNA. Translation: AAH35315.1.
    BC047758 mRNA. Translation: AAH47758.2.
    BC064423 mRNA. Translation: AAH64423.1.
    CCDSiCCDS2002.2. [Q86YJ6-1]
    CCDS58718.1. [Q86YJ6-2]
    RefSeqiNP_001231605.1. NM_001244676.1. [Q86YJ6-2]
    NP_060741.3. NM_018271.4. [Q86YJ6-1]
    XP_005264457.1. XM_005264400.2. [Q86YJ6-1]
    XP_005264458.1. XM_005264401.2. [Q86YJ6-1]
    XP_005264460.1. XM_005264403.2. [Q86YJ6-3]
    XP_006712106.1. XM_006712043.1. [Q86YJ6-2]
    UniGeneiHs.739080.
    Hs.740434.

    Genome annotation databases

    EnsembliENST00000324166; ENSP00000327323; ENSG00000144115. [Q86YJ6-1]
    ENST00000343544; ENSP00000339563; ENSG00000144115. [Q86YJ6-2]
    ENST00000377254; ENSP00000366464; ENSG00000144115. [Q86YJ6-3]
    GeneIDi55258.
    KEGGihsa:55258.
    UCSCiuc002ssw.4. human. [Q86YJ6-2]
    uc002ssy.4. human. [Q86YJ6-1]

    Polymorphism databases

    DMDMi313104279.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HM185274 mRNA. Translation: ADL14696.1 .
    AK001778 mRNA. Translation: BAA91904.1 . Different initiation.
    AK095303 mRNA. Translation: BAG53023.1 .
    AC092836 Genomic DNA. Translation: AAX88906.1 . Sequence problems.
    BC035315 mRNA. Translation: AAH35315.1 .
    BC047758 mRNA. Translation: AAH47758.2 .
    BC064423 mRNA. Translation: AAH64423.1 .
    CCDSi CCDS2002.2. [Q86YJ6-1 ]
    CCDS58718.1. [Q86YJ6-2 ]
    RefSeqi NP_001231605.1. NM_001244676.1. [Q86YJ6-2 ]
    NP_060741.3. NM_018271.4. [Q86YJ6-1 ]
    XP_005264457.1. XM_005264400.2. [Q86YJ6-1 ]
    XP_005264458.1. XM_005264401.2. [Q86YJ6-1 ]
    XP_005264460.1. XM_005264403.2. [Q86YJ6-3 ]
    XP_006712106.1. XM_006712043.1. [Q86YJ6-2 ]
    UniGenei Hs.739080.
    Hs.740434.

    3D structure databases

    ProteinModelPortali Q86YJ6.
    SMRi Q86YJ6. Positions 3-428.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120549. 1 interaction.
    IntActi Q86YJ6. 2 interactions.
    MINTi MINT-1391634.

    Polymorphism databases

    DMDMi 313104279.

    Proteomic databases

    MaxQBi Q86YJ6.
    PaxDbi Q86YJ6.
    PRIDEi Q86YJ6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000324166 ; ENSP00000327323 ; ENSG00000144115 . [Q86YJ6-1 ]
    ENST00000343544 ; ENSP00000339563 ; ENSG00000144115 . [Q86YJ6-2 ]
    ENST00000377254 ; ENSP00000366464 ; ENSG00000144115 . [Q86YJ6-3 ]
    GeneIDi 55258.
    KEGGi hsa:55258.
    UCSCi uc002ssw.4. human. [Q86YJ6-2 ]
    uc002ssy.4. human. [Q86YJ6-1 ]

    Organism-specific databases

    CTDi 55258.
    GeneCardsi GC02P088469.
    H-InvDB HIX0002247.
    HGNCi HGNC:25602. THNSL2.
    HPAi HPA035395.
    MIMi 611261. gene.
    neXtProti NX_Q86YJ6.
    PharmGKBi PA162405692.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0498.
    HOVERGENi HBG108568.
    InParanoidi Q86YJ6.
    OMAi WRSHALN.
    OrthoDBi EOG7QZG93.
    PhylomeDBi Q86YJ6.
    TreeFami TF329641.

    Enzyme and pathway databases

    SignaLinki Q86YJ6.

    Miscellaneous databases

    GenomeRNAii 55258.
    NextBioi 59344.
    PROi Q86YJ6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86YJ6.
    Bgeei Q86YJ6.
    CleanExi HS_THNSL2.
    Genevestigatori Q86YJ6.

    Family and domain databases

    Gene3Di 3.90.1380.10. 1 hit.
    InterProi IPR029144. Thr_synth_N.
    IPR004450. Thr_synthase_like.
    IPR001926. Trp_syn_b_sub_like_PLP_eny_SF.
    IPR027457. TSH2.
    [Graphical view ]
    PANTHERi PTHR10314:SF89. PTHR10314:SF89. 1 hit.
    Pfami PF00291. PALP. 1 hit.
    PF14821. Thr_synth_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53686. SSF53686. 1 hit.
    TIGRFAMsi TIGR00260. thrC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel T cell cytokine, secreted osteoclastogenic factor of activated T cells, induces osteoclast formation in a RANKL-independent manner."
      Rifas L., Weitzmann M.N.
      Arthritis Rheum. 60:3324-3335(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOLAT), FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Peripheral blood T-cell.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-484 (ISOFORM 4), VARIANT GLU-41.
      Tissue: Ovarian carcinoma and Tongue.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-484 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 140-431 (ISOFORM 3), VARIANTS GLU-41 AND GLY-324.
      Tissue: Brain, Colon and Skin.

    Entry informationi

    Entry nameiTHNS2_HUMAN
    AccessioniPrimary (citable) accession number: Q86YJ6
    Secondary accession number(s): B3KTB1
    , B5MDX8, B7WPF8, D9ZZB8, Q6P2M7, Q6PI27, Q9NV54
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 81 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3