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Protein

Threonine synthase-like 2

Gene

THNSL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Isoform 1: Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate (By similarity).By similarity
Isoform SOFAT: Potent inducer of osteoblastic production of IL6. May act to exacerbate inflammation and/or bone turnover under inflammatory conditions.1 Publication

Cofactori

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. pyridoxal phosphate binding Source: BHF-UCL
  3. serine binding Source: BHF-UCL

GO - Biological processi

  1. 2-oxobutyrate biosynthetic process Source: BHF-UCL
  2. dephosphorylation Source: BHF-UCL
  3. serine family amino acid catabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

SignaLinkiQ86YJ6.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine synthase-like 2 (EC:4.2.3.-)
Short name:
TSH2
Alternative name(s):
Secreted osteoclastogenic factor of activated T-cells
Short name:
SOFAT
Gene namesi
Name:THNSL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:25602. THNSL2.

Subcellular locationi

Isoform SOFAT : Secreted
Note: Secreted by activated T-cells via a calcineurin-independent pathway.

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162405692.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Threonine synthase-like 2PRO_0000306407Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei113 – 1131N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiQ86YJ6.
PaxDbiQ86YJ6.
PRIDEiQ86YJ6.

Expressioni

Gene expression databases

BgeeiQ86YJ6.
CleanExiHS_THNSL2.
ExpressionAtlasiQ86YJ6. baseline and differential.
GenevestigatoriQ86YJ6.

Organism-specific databases

HPAiHPA035395.

Interactioni

Protein-protein interaction databases

BioGridi120549. 1 interaction.
IntActiQ86YJ6. 2 interactions.
MINTiMINT-1391634.

Structurei

3D structure databases

ProteinModelPortaliQ86YJ6.
SMRiQ86YJ6. Positions 3-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the threonine synthase family.Curated

Phylogenomic databases

eggNOGiCOG0498.
GeneTreeiENSGT00530000063879.
HOVERGENiHBG108568.
InParanoidiQ86YJ6.
OMAiRRKNWQL.
OrthoDBiEOG7QZG93.
PhylomeDBiQ86YJ6.
TreeFamiTF329641.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
IPR027457. TSH2.
[Graphical view]
PANTHERiPTHR10314:SF89. PTHR10314:SF89. 1 hit.
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q86YJ6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS
60 70 80 90 100
YPGLVKELCA LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN
110 120 130 140 150
VLELWHGVTY AFKDLSLSCT TQFLQYFLEK REKHVTVVVG TSGDTGSAAI
160 170 180 190 200
ESVQGAKNMD IIVLLPKGHC TKIQELQMTT VLKQNVHVFG VEGNSDELDE
210 220 230 240 250
PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF QCTPSLDTHP
260 270 280 290 300
LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF
310 320 330 340 350
SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV
360 370 380 390 400
NLPKELHSKL SEAVTSVSVS DEAITQTMGR CWDENQYLLC PHSAVAVNYH
410 420 430 440 450
YQQIDRQQPS TPRCCLAPAS AAKFPEAVLA AGLTPETPAE IVALEHKETR
460 470 480
CTLMRRGDNW MLMLRDTIED LSRQWRSHAL NTSQ
Length:484
Mass (Da):54,116
Last modified:November 30, 2010 - v3
Checksum:iF177B2B7DE6E8752
GO
Isoform 2 (identifier: Q86YJ6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGG

Note: No experimental confirmation available.

Show »
Length:405
Mass (Da):45,037
Checksum:i5A2B03980DD1B3ED
GO
Isoform 3 (identifier: Q86YJ6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-484: LSEAVTSVSV...WRSHALNTSQ → HSPVLPRPCLCSQVPGSCPGCWPDP

Note: No experimental confirmation available.

Show »
Length:384
Mass (Da):42,718
Checksum:iBE36DE86123D5733
GO
Isoform 4 (identifier: Q86YJ6-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-274: Missing.

Note: No experimental confirmation available.

Show »
Length:423
Mass (Da):47,428
Checksum:i14A1A6D8308BC93F
GO
Isoform SOFAT (identifier: Q86YJ6-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGD

Show »
Length:247
Mass (Da):27,414
Checksum:i0124469439FCFD9D
GO

Sequence cautioni

The sequence AAX88906.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAA91904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631I → V in BAA91904. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti41 – 411G → E.2 Publications
Corresponds to variant rs4129190 [ dbSNP | Ensembl ].
VAR_054635
Natural varianti108 – 1081V → I.
Corresponds to variant rs35541720 [ dbSNP | Ensembl ].
VAR_054636
Natural varianti204 – 2041T → N.
Corresponds to variant rs34136143 [ dbSNP | Ensembl ].
VAR_054637
Natural varianti324 – 3241R → G.1 Publication
Corresponds to variant rs17855905 [ dbSNP | Ensembl ].
VAR_054638

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 158158Missing in isoform SOFAT. 1 PublicationVSP_041659Add
BLAST
Alternative sequencei214 – 27461Missing in isoform 4. 1 PublicationVSP_028468Add
BLAST
Alternative sequencei360 – 484125LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGG in isoform 2. 1 PublicationVSP_028469Add
BLAST
Alternative sequencei360 – 484125LSEAV…LNTSQ → HSPVLPRPCLCSQVPGSCPG CWPDP in isoform 3. 1 PublicationVSP_028470Add
BLAST
Alternative sequencei360 – 484125LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGD in isoform SOFAT. 1 PublicationVSP_041660Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM185274 mRNA. Translation: ADL14696.1.
AK001778 mRNA. Translation: BAA91904.1. Different initiation.
AK095303 mRNA. Translation: BAG53023.1.
AC092836 Genomic DNA. Translation: AAX88906.1. Sequence problems.
BC035315 mRNA. Translation: AAH35315.1.
BC047758 mRNA. Translation: AAH47758.2.
BC064423 mRNA. Translation: AAH64423.1.
CCDSiCCDS2002.2. [Q86YJ6-1]
CCDS58718.1. [Q86YJ6-2]
RefSeqiNP_001231605.1. NM_001244676.1. [Q86YJ6-2]
NP_060741.3. NM_018271.4. [Q86YJ6-1]
XP_005264457.1. XM_005264400.2. [Q86YJ6-1]
XP_005264458.1. XM_005264401.2. [Q86YJ6-1]
XP_005264460.1. XM_005264403.2. [Q86YJ6-3]
XP_006712106.1. XM_006712043.1. [Q86YJ6-2]
UniGeneiHs.739080.
Hs.740434.

Genome annotation databases

EnsembliENST00000324166; ENSP00000327323; ENSG00000144115. [Q86YJ6-1]
ENST00000343544; ENSP00000339563; ENSG00000144115. [Q86YJ6-2]
GeneIDi55258.
KEGGihsa:55258.
UCSCiuc002ssw.4. human. [Q86YJ6-2]
uc002ssy.4. human. [Q86YJ6-1]

Polymorphism databases

DMDMi313104279.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM185274 mRNA. Translation: ADL14696.1.
AK001778 mRNA. Translation: BAA91904.1. Different initiation.
AK095303 mRNA. Translation: BAG53023.1.
AC092836 Genomic DNA. Translation: AAX88906.1. Sequence problems.
BC035315 mRNA. Translation: AAH35315.1.
BC047758 mRNA. Translation: AAH47758.2.
BC064423 mRNA. Translation: AAH64423.1.
CCDSiCCDS2002.2. [Q86YJ6-1]
CCDS58718.1. [Q86YJ6-2]
RefSeqiNP_001231605.1. NM_001244676.1. [Q86YJ6-2]
NP_060741.3. NM_018271.4. [Q86YJ6-1]
XP_005264457.1. XM_005264400.2. [Q86YJ6-1]
XP_005264458.1. XM_005264401.2. [Q86YJ6-1]
XP_005264460.1. XM_005264403.2. [Q86YJ6-3]
XP_006712106.1. XM_006712043.1. [Q86YJ6-2]
UniGeneiHs.739080.
Hs.740434.

3D structure databases

ProteinModelPortaliQ86YJ6.
SMRiQ86YJ6. Positions 3-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120549. 1 interaction.
IntActiQ86YJ6. 2 interactions.
MINTiMINT-1391634.

Polymorphism databases

DMDMi313104279.

Proteomic databases

MaxQBiQ86YJ6.
PaxDbiQ86YJ6.
PRIDEiQ86YJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324166; ENSP00000327323; ENSG00000144115. [Q86YJ6-1]
ENST00000343544; ENSP00000339563; ENSG00000144115. [Q86YJ6-2]
GeneIDi55258.
KEGGihsa:55258.
UCSCiuc002ssw.4. human. [Q86YJ6-2]
uc002ssy.4. human. [Q86YJ6-1]

Organism-specific databases

CTDi55258.
GeneCardsiGC02P088469.
H-InvDBHIX0002247.
HGNCiHGNC:25602. THNSL2.
HPAiHPA035395.
MIMi611261. gene.
neXtProtiNX_Q86YJ6.
PharmGKBiPA162405692.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0498.
GeneTreeiENSGT00530000063879.
HOVERGENiHBG108568.
InParanoidiQ86YJ6.
OMAiRRKNWQL.
OrthoDBiEOG7QZG93.
PhylomeDBiQ86YJ6.
TreeFamiTF329641.

Enzyme and pathway databases

SignaLinkiQ86YJ6.

Miscellaneous databases

ChiTaRSiTHNSL2. human.
GenomeRNAii55258.
NextBioi59344.
PROiQ86YJ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ86YJ6.
CleanExiHS_THNSL2.
ExpressionAtlasiQ86YJ6. baseline and differential.
GenevestigatoriQ86YJ6.

Family and domain databases

Gene3Di3.90.1380.10. 1 hit.
InterProiIPR029144. Thr_synth_N.
IPR004450. Thr_synthase_like.
IPR001926. TrpB-like_PLP-dep.
IPR027457. TSH2.
[Graphical view]
PANTHERiPTHR10314:SF89. PTHR10314:SF89. 1 hit.
PfamiPF00291. PALP. 1 hit.
PF14821. Thr_synth_N. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00260. thrC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel T cell cytokine, secreted osteoclastogenic factor of activated T cells, induces osteoclast formation in a RANKL-independent manner."
    Rifas L., Weitzmann M.N.
    Arthritis Rheum. 60:3324-3335(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOFAT), FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Peripheral blood T-cell.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-484 (ISOFORM 4), VARIANT GLU-41.
    Tissue: Ovarian carcinoma and Tongue.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-484 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 140-431 (ISOFORM 3), VARIANTS GLU-41 AND GLY-324.
    Tissue: Brain, Colon and Skin.

Entry informationi

Entry nameiTHNS2_HUMAN
AccessioniPrimary (citable) accession number: Q86YJ6
Secondary accession number(s): B3KTB1
, B5MDX8, B7WPF8, D9ZZB8, Q6P2M7, Q6PI27, Q9NV54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: November 30, 2010
Last modified: February 4, 2015
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.