Q86YJ6 (THNS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine synthase-like 2
Short name=TSH2
EC=4.2.3.-

Alternative name(s):
Secreted osteoclastogenic factor of activated T cells
Short name=SOFAT

Gene names

Name:

THNSL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Isoform 1: Acts as a catabolic phospho-lyase on both gamma- and beta-phosphorylated substrates. Degrades O-phospho-threonine (PThr) to alpha-ketobutyrate, ammonia and phosphate By similarity. Ref.1 Isoform SOLAT: Potent inducer of osteoblastic production of IL6. May act to exacerbate inflammation and/or bone turnover under inflammatory conditions. Ref.1
Cofactor	Pyridoxal phosphate By similarity.
Subcellular location	Isoform SOLAT: Secreted. Note: Secreted by activated T cells via a calcineurin-independent pathway. Ref.1
Sequence similarities	Belongs to the threonine synthase family.
Sequence caution	The sequence AAX88906.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAA91904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Pyridoxal phosphate
Molecular function	Cytokine Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	2-oxobutyrate biosynthetic process Inferred from sequence or structural similarity. Source: BHF-UCL dephosphorylation Inferred from sequence or structural similarity. Source: BHF-UCL serine family amino acid catabolic process Inferred from sequence or structural similarity. Source: BHF-UCL threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate binding Inferred from sequence or structural similarity. Source: BHF-UCL serine binding Inferred from sequence or structural similarity. Source: BHF-UCL threonine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86YJ6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86YJ6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGG

Note: No experimental confirmation available.

Isoform 3 (identifier: Q86YJ6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     360-484: LSEAVTSVSV...WRSHALNTSQ → HSPVLPRPCLCSQVPGSCPGCWPDP

Note: No experimental confirmation available.

Isoform 4 (identifier: Q86YJ6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     214-274: Missing.

Note: No experimental confirmation available.

Isoform SOLAT (identifier: Q86YJ6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     360-484: LSEAVTSVSV...WRSHALNTSQ → WERQDYEKMA...PGTAMVRGGD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Threonine synthase-like 2		PRO_0000306407
Amino acid modifications
	Modified residue	113	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Alternative sequence	1 – 158	158	Missing in isoform SOLAT.		VSP_041659
	Alternative sequence	214 – 274	61	Missing in isoform 4.		VSP_028468
	Alternative sequence	360 – 484	125	LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGG in isoform 2.		VSP_028469
	Alternative sequence	360 – 484	125	LSEAV…LNTSQ → HSPVLPRPCLCSQVPGSCPG CWPDP in isoform 3.		VSP_028470
	Alternative sequence	360 – 484	125	LSEAV…LNTSQ → WERQDYEKMAVMECDGCCVE LCLGNCGPRRGSVTDIPGTA MVRGGD in isoform SOLAT.		VSP_041660
	Natural variant	41	1	G → E. Ref.2 Ref.4 Corresponds to variant rs4129190 [ dbSNP | Ensembl ].		VAR_054635
	Natural variant	108	1	V → I. Corresponds to variant rs35541720 [ dbSNP | Ensembl ].		VAR_054636
	Natural variant	204	1	T → N. Corresponds to variant rs34136143 [ dbSNP | Ensembl ].		VAR_054637
	Natural variant	324	1	R → G. Ref.4 Corresponds to variant rs17855905 [ dbSNP | Ensembl ].		VAR_054638
Experimental info
	Sequence conflict	63	1	I → V in BAA91904. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: F177B2B7DE6E8752	FASTA	484	54,116
	10 20 30 40 50 60 MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS YPGLVKELCA 70 80 90 100 110 120 LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN VLELWHGVTY AFKDLSLSCT 130 140 150 160 170 180 TQFLQYFLEK REKHVTVVVG TSGDTGSAAI ESVQGAKNMD IIVLLPKGHC TKIQELQMTT 190 200 210 220 230 240 VLKQNVHVFG VEGNSDELDE PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF 250 260 270 280 290 300 QCTPSLDTHP LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF 310 320 330 340 350 360 SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV NLPKELHSKL 370 380 390 400 410 420 SEAVTSVSVS DEAITQTMGR CWDENQYLLC PHSAVAVNYH YQQIDRQQPS TPRCCLAPAS 430 440 450 460 470 480 AAKFPEAVLA AGLTPETPAE IVALEHKETR CTLMRRGDNW MLMLRDTIED LSRQWRSHAL NTSQ
	Isoform 2 [UniParc]. Checksum: 5A2B03980DD1B3ED	FASTA	405	45,037
	10 20 30 40 50 60 MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS YPGLVKELCA 70 80 90 100 110 120 LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN VLELWHGVTY AFKDLSLSCT 130 140 150 160 170 180 TQFLQYFLEK REKHVTVVVG TSGDTGSAAI ESVQGAKNMD IIVLLPKGHC TKIQELQMTT 190 200 210 220 230 240 VLKQNVHVFG VEGNSDELDE PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF 250 260 270 280 290 300 QCTPSLDTHP LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF 310 320 330 340 350 360 SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV NLPKELHSKW 370 380 390 400 ERQDYEKMAV MECDGCCVEL CLGNCGPRRG SVTDIPGTAM VRGGG
	Isoform 3 [UniParc]. Checksum: BE36DE86123D5733	FASTA	384	42,718
	10 20 30 40 50 60 MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS YPGLVKELCA 70 80 90 100 110 120 LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN VLELWHGVTY AFKDLSLSCT 130 140 150 160 170 180 TQFLQYFLEK REKHVTVVVG TSGDTGSAAI ESVQGAKNMD IIVLLPKGHC TKIQELQMTT 190 200 210 220 230 240 VLKQNVHVFG VEGNSDELDE PIKTVFADVA FVKKHNLMSL NSINWSRVLV QMAHHFFAYF 250 260 270 280 290 300 QCTPSLDTHP LPLVEVVVPT GAAGNLAAGY IAQKIGLPIR LVVAVNRNDI IHRTVQQGDF 310 320 330 340 350 360 SLSEAVKSTL ASAMDIQVPY NMERVFWLLS GSDSQVTRAL MEQFERTQSV NLPKELHSKH 370 380 SPVLPRPCLC SQVPGSCPGC WPDP
	Isoform 4 [UniParc]. Checksum: 14A1A6D8308BC93F	FASTA	423	47,428
	10 20 30 40 50 60 MWYVSTRGVA PRVNFEGALF SGYAPDGGLF MPEELPQLDR GTLCQWSTLS YPGLVKELCA 70 80 90 100 110 120 LFIGSELLPK DELNDLIDRA FSRFRHREVV HLSRLRNGLN VLELWHGVTY AFKDLSLSCT 130 140 150 160 170 180 TQFLQYFLEK REKHVTVVVG TSGDTGSAAI ESVQGAKNMD IIVLLPKGHC TKIQELQMTT 190 200 210 220 230 240 VLKQNVHVFG VEGNSDELDE PIKTVFADVA FVKIGLPIRL VVAVNRNDII HRTVQQGDFS 250 260 270 280 290 300 LSEAVKSTLA SAMDIQVPYN MERVFWLLSG SDSQVTRALM EQFERTQSVN LPKELHSKLS 310 320 330 340 350 360 EAVTSVSVSD EAITQTMGRC WDENQYLLCP HSAVAVNYHY QQIDRQQPST PRCCLAPASA 370 380 390 400 410 420 AKFPEAVLAA GLTPETPAEI VALEHKETRC TLMRRGDNWM LMLRDTIEDL SRQWRSHALN TSQ
	Isoform SOLAT [UniParc]. Checksum: 0124469439FCFD9D	FASTA	247	27,414
	10 20 30 40 50 60 MDIIVLLPKG HCTKIQELQM TTVLKQNVHV FGVEGNSDEL DEPIKTVFAD VAFVKKHNLM 70 80 90 100 110 120 SLNSINWSRV LVQMAHHFFA YFQCTPSLDT HPLPLVEVVV PTGAAGNLAA GYIAQKIGLP 130 140 150 160 170 180 IRLVVAVNRN DIIHRTVQQG DFSLSEAVKS TLASAMDIQV PYNMERVFWL LSGSDSQVTR 190 200 210 220 230 240 ALMEQFERTQ SVNLPKELHS KWERQDYEKM AVMECDGCCV ELCLGNCGPR RGSVTDIPGT AMVRGGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel T cell cytokine, secreted osteoclastogenic factor of activated T cells, induces osteoclast formation in a RANKL-independent manner." Rifas L., Weitzmann M.N. Arthritis Rheum. 60:3324-3335(2009) [PubMed: 19877052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SOLAT), FUNCTION, SUBCELLULAR LOCATION. Tissue: Peripheral blood T-cell.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-484 (ISOFORM 4), VARIANT GLU-41. Tissue: Ovarian carcinoma and Tongue.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-484 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 140-431 (ISOFORM 3), VARIANTS GLU-41 AND GLY-324. Tissue: Brain, Colon and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HM185274 mRNA. Translation: ADL14696.1. AK001778 mRNA. Translation: BAA91904.1. Different initiation. AK095303 mRNA. Translation: BAG53023.1. AC092836 Genomic DNA. Translation: AAX88906.1. Sequence problems. BC035315 mRNA. Translation: AAH35315.1. BC047758 mRNA. Translation: AAH47758.2. BC064423 mRNA. Translation: AAH64423.1.
IPI	IPI00019452. IPI00395997. IPI00413065. IPI00448531. IPI01021494.
RefSeq	NP_001231605.1. NM_001244676.1. NP_060741.3. NM_018271.4.
UniGene	Hs.516179.
3D structure databases
HSSP	HSSP built from PDB template 1KL7 based on UniProtKB P16120.
ProteinModelPortal	Q86YJ6.
SMR	Q86YJ6. Positions 1-474.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86YJ6. 2 interactions.
MINT	MINT-1391634.
STRING	Q86YJ6.
Polymorphism databases
DMDM	158706354.
Proteomic databases
PRIDE	Q86YJ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000324166; ENSP00000327323; ENSG00000144115. ENST00000358591; ENSP00000351402; ENSG00000144115.
GeneID	55258.
KEGG	hsa:55258.
UCSC	uc002ssw.2. human. uc002ssy.2. human.
Organism-specific databases
CTD	55258.
GeneCards	GC02P088469.
HGNC	HGNC:25602. THNSL2.
HPA	HPA035395.
MIM	611261. gene.
neXtProt	NX_Q86YJ6.
PharmGKB	PA162405692.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18251.
GeneTree	ENSGT00530000063879.
HOGENOM	HBG521025.
HOVERGEN	HBG108568.
InParanoid	Q86YJ6.
OMA	HNDIIHR.
PhylomeDB	Q86YJ6.
Gene expression databases
ArrayExpress	Q86YJ6.
Bgee	Q86YJ6.
CleanEx	HS_THNSL2.
Genevestigator	Q86YJ6.
Family and domain databases
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR004450. Thr_synthase. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR00260. ThrC. 1 hit.
ProtoNet	Search...
Other
NextBio	59344.
SOURCE	Search...

Entry information

Entry name

THNS2_HUMAN

Accession

Primary (citable) accession number: Q86YJ6
Secondary accession number(s): B3KTB1 , B5MDX8, B7WPF8, D9ZZB8, Q6P2M7, Q6PI27, Q9NV54

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	November 30, 2010
Last modified:	January 25, 2012
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.