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Protein

E3 ubiquitin-protein ligase MARCH9

Gene

MARCH9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri102 – 162RING-CH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH9 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 9
Membrane-associated RING-CH protein IX
Short name:
MARCH-IX
RING finger protein 179
RING-type E3 ubiquitin transferase MARCH9Curated
Gene namesi
Name:MARCH9
Synonyms:RNF179
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139266.5.
HGNCiHGNC:25139. MARCH9.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi231D → N: Diminishes ability to promote MHC-I internalization. 1 Publication1

Organism-specific databases

DisGeNETi92979.
OpenTargetsiENSG00000139266.
PharmGKBiPA134920045.

Polymorphism and mutation databases

BioMutaiMARCH9.
DMDMi74759533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002742821 – 346E3 ubiquitin-protein ligase MARCH9Add BLAST346

Proteomic databases

PaxDbiQ86YJ5.
PeptideAtlasiQ86YJ5.
PRIDEiQ86YJ5.

PTM databases

iPTMnetiQ86YJ5.
PhosphoSitePlusiQ86YJ5.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000139266.
CleanExiHS_MARCH9.
GenevisibleiQ86YJ5. HS.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi124992. 4 interactors.
IntActiQ86YJ5. 3 interactors.
MINTiMINT-4053156.
STRINGi9606.ENSP00000266643.

Structurei

3D structure databases

ProteinModelPortaliQ86YJ5.
SMRiQ86YJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 89Pro-richAdd BLAST16

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri102 – 162RING-CH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110738.
HOGENOMiHOG000113484.
HOVERGENiHBG052412.
InParanoidiQ86YJ5.
KOiK10659.
OMAiSRCCVLL.
OrthoDBiEOG091G0JT5.
PhylomeDBiQ86YJ5.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiView protein in Pfam
PF12906. RINGv. 1 hit.
SMARTiView protein in SMART
SM00744. RINGv. 1 hit.
PROSITEiView protein in PROSITE
PS51292. ZF_RING_CH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YJ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKSRLRMFL NELKLLVLTG GGRPRAEPQP RGGRGGGCGW APFAGCSTRD
60 70 80 90 100
GDGDEEEYYG SEPRARGLAG DKEPRAGPLP PPAPPLPPPG ALDALSLSSS
110 120 130 140 150
LDSGLRTPQC RICFQGPEQG ELLSPCRCDG SVRCTHQPCL IRWISERGSW
160 170 180 190 200
SCELCYFKYQ VLAISTKNPL QWQAISLTVI EKVQIAAIVL GSLFLVASIS
210 220 230 240 250
WLIWSSLSPS AKWQRQDLLF QICYGMYGFM DVVCIGLIIH EGSSVYRIFK
260 270 280 290 300
RWQAVNQQWK VLNYDKTKDI GGDAGGGTAG KSGPRNSRTG PTSGATSRPP
310 320 330 340
AAQRMRTLLP QRCGYTILHL LGQLRPPDAR SSSHSGREVV MRVTTV
Length:346
Mass (Da):37,772
Last modified:March 1, 2004 - v2
Checksum:iFA6142B973A359B8
GO
Isoform 2 (identifier: Q86YJ5-2) [UniParc]FASTAAdd to basket
Also known as: RINGless

The sequence of this isoform differs from the canonical sequence as follows:
     1-170: MLKSRLRMFL...VLAISTKNPL → MVSDKCHILD...LGLFGALSPT

Note: Has no E3 activity due to lack of RINGv-type zinc finger domain but is able to dimerize with and stabilize isoform 1.
Show »
Length:233
Mass (Da):25,873
Checksum:iF8C58F644D827B1C
GO

Sequence cautioni

The sequence AAH50397 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030246257Q → H1 PublicationCorresponds to variant dbSNP:rs17856312Ensembl.1
Natural variantiVAR_030247307T → P1 PublicationCorresponds to variant dbSNP:rs17850517Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0226971 – 170MLKSR…TKNPL → MVSDKCHILDLIKAQRRQML ILPEGFREGSWSTLLFRATH HLALHPFLGLFGALSPT in isoform 2. 2 PublicationsAdd BLAST170

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK313925 mRNA. Translation: BAG36646.1.
CH471054 Genomic DNA. Translation: EAW97065.1.
BC009489 mRNA. Translation: AAH09489.1.
BC036455 mRNA. Translation: AAH36455.2.
BC050397 mRNA. Translation: AAH50397.1. Different initiation.
CCDSiCCDS31847.1. [Q86YJ5-1]
RefSeqiNP_612405.2. NM_138396.5. [Q86YJ5-1]
UniGeneiHs.632709.

Genome annotation databases

EnsembliENST00000266643; ENSP00000266643; ENSG00000139266. [Q86YJ5-1]
ENST00000548358; ENSP00000446758; ENSG00000139266. [Q86YJ5-2]
GeneIDi92979.
KEGGihsa:92979.
UCSCiuc001spx.3. human. [Q86YJ5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMARH9_HUMAN
AccessioniPrimary (citable) accession number: Q86YJ5
Secondary accession number(s): B2R9U9, Q86VN5, Q96GG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: March 1, 2004
Last modified: September 27, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways