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Protein

E3 ubiquitin-protein ligase MARCH9

Gene

MARCH9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri102 – 16261RING-CH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH9 (EC:6.3.2.-)
Alternative name(s):
Membrane-associated RING finger protein 9
Membrane-associated RING-CH protein IX
Short name:
MARCH-IX
RING finger protein 179
Gene namesi
Name:MARCH9
Synonyms:RNF179
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:25139. MARCH9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • Golgi stack Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi231 – 2311D → N: Diminishes ability to promote MHC-I internalization. 1 Publication

Organism-specific databases

PharmGKBiPA134920045.

Polymorphism and mutation databases

BioMutaiMARCH9.
DMDMi74759533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346E3 ubiquitin-protein ligase MARCH9PRO_0000274282Add
BLAST

Proteomic databases

PaxDbiQ86YJ5.
PeptideAtlasiQ86YJ5.
PRIDEiQ86YJ5.

PTM databases

iPTMnetiQ86YJ5.
PhosphoSiteiQ86YJ5.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000139266.
CleanExiHS_MARCH9.
GenevisibleiQ86YJ5. HS.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi124992. 5 interactions.
IntActiQ86YJ5. 3 interactions.
MINTiMINT-4053156.
STRINGi9606.ENSP00000266643.

Structurei

3D structure databases

ProteinModelPortaliQ86YJ5.
SMRiQ86YJ5. Positions 110-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi74 – 8916Pro-richAdd
BLAST

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Sequence similaritiesi

Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri102 – 16261RING-CH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110738.
HOGENOMiHOG000113484.
HOVERGENiHBG052412.
InParanoidiQ86YJ5.
KOiK10659.
OMAiCSKGKLE.
OrthoDBiEOG091G0JT5.
PhylomeDBiQ86YJ5.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86YJ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKSRLRMFL NELKLLVLTG GGRPRAEPQP RGGRGGGCGW APFAGCSTRD
60 70 80 90 100
GDGDEEEYYG SEPRARGLAG DKEPRAGPLP PPAPPLPPPG ALDALSLSSS
110 120 130 140 150
LDSGLRTPQC RICFQGPEQG ELLSPCRCDG SVRCTHQPCL IRWISERGSW
160 170 180 190 200
SCELCYFKYQ VLAISTKNPL QWQAISLTVI EKVQIAAIVL GSLFLVASIS
210 220 230 240 250
WLIWSSLSPS AKWQRQDLLF QICYGMYGFM DVVCIGLIIH EGSSVYRIFK
260 270 280 290 300
RWQAVNQQWK VLNYDKTKDI GGDAGGGTAG KSGPRNSRTG PTSGATSRPP
310 320 330 340
AAQRMRTLLP QRCGYTILHL LGQLRPPDAR SSSHSGREVV MRVTTV
Length:346
Mass (Da):37,772
Last modified:March 1, 2004 - v2
Checksum:iFA6142B973A359B8
GO
Isoform 2 (identifier: Q86YJ5-2) [UniParc]FASTAAdd to basket
Also known as: RINGless

The sequence of this isoform differs from the canonical sequence as follows:
     1-170: MLKSRLRMFL...VLAISTKNPL → MVSDKCHILD...LGLFGALSPT

Note: Has no E3 activity due to lack of RINGv-type zinc finger domain but is able to dimerize with and stabilize isoform 1.
Show »
Length:233
Mass (Da):25,873
Checksum:iF8C58F644D827B1C
GO

Sequence cautioni

The sequence AAH50397 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti257 – 2571Q → H.1 Publication
Corresponds to variant rs17856312 [ dbSNP | Ensembl ].
VAR_030246
Natural varianti307 – 3071T → P.1 Publication
Corresponds to variant rs17850517 [ dbSNP | Ensembl ].
VAR_030247

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 170170MLKSR…TKNPL → MVSDKCHILDLIKAQRRQML ILPEGFREGSWSTLLFRATH HLALHPFLGLFGALSPT in isoform 2. 2 PublicationsVSP_022697Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK313925 mRNA. Translation: BAG36646.1.
CH471054 Genomic DNA. Translation: EAW97065.1.
BC009489 mRNA. Translation: AAH09489.1.
BC036455 mRNA. Translation: AAH36455.2.
BC050397 mRNA. Translation: AAH50397.1. Different initiation.
CCDSiCCDS31847.1. [Q86YJ5-1]
RefSeqiNP_612405.2. NM_138396.5. [Q86YJ5-1]
UniGeneiHs.632709.

Genome annotation databases

EnsembliENST00000266643; ENSP00000266643; ENSG00000139266. [Q86YJ5-1]
ENST00000548358; ENSP00000446758; ENSG00000139266. [Q86YJ5-2]
GeneIDi92979.
KEGGihsa:92979.
UCSCiuc001spx.3. human. [Q86YJ5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK313925 mRNA. Translation: BAG36646.1.
CH471054 Genomic DNA. Translation: EAW97065.1.
BC009489 mRNA. Translation: AAH09489.1.
BC036455 mRNA. Translation: AAH36455.2.
BC050397 mRNA. Translation: AAH50397.1. Different initiation.
CCDSiCCDS31847.1. [Q86YJ5-1]
RefSeqiNP_612405.2. NM_138396.5. [Q86YJ5-1]
UniGeneiHs.632709.

3D structure databases

ProteinModelPortaliQ86YJ5.
SMRiQ86YJ5. Positions 110-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124992. 5 interactions.
IntActiQ86YJ5. 3 interactions.
MINTiMINT-4053156.
STRINGi9606.ENSP00000266643.

PTM databases

iPTMnetiQ86YJ5.
PhosphoSiteiQ86YJ5.

Polymorphism and mutation databases

BioMutaiMARCH9.
DMDMi74759533.

Proteomic databases

PaxDbiQ86YJ5.
PeptideAtlasiQ86YJ5.
PRIDEiQ86YJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000266643; ENSP00000266643; ENSG00000139266. [Q86YJ5-1]
ENST00000548358; ENSP00000446758; ENSG00000139266. [Q86YJ5-2]
GeneIDi92979.
KEGGihsa:92979.
UCSCiuc001spx.3. human. [Q86YJ5-1]

Organism-specific databases

CTDi92979.
GeneCardsiMARCH9.
HGNCiHGNC:25139. MARCH9.
MIMi613336. gene.
neXtProtiNX_Q86YJ5.
PharmGKBiPA134920045.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110738.
HOGENOMiHOG000113484.
HOVERGENiHBG052412.
InParanoidiQ86YJ5.
KOiK10659.
OMAiCSKGKLE.
OrthoDBiEOG091G0JT5.
PhylomeDBiQ86YJ5.
TreeFamiTF319557.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiMARCH9. human.
GenomeRNAii92979.
PROiQ86YJ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139266.
CleanExiHS_MARCH9.
GenevisibleiQ86YJ5. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMARH9_HUMAN
AccessioniPrimary (citable) accession number: Q86YJ5
Secondary accession number(s): B2R9U9, Q86VN5, Q96GG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.