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Protein

Kinesin-like protein KIF18B

Gene

KIF18B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In complex with KIF2C, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Its major role may be to transport KIF2C and/or MAPRE1 along microtubules.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi118 – 1258ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
  • kinesin binding Source: UniProtKB
  • motor activity Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • microtubule-based movement Source: GO_Central
  • microtubule depolymerization Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • mitotic sister chromatid segregation Source: GO_Central
  • regulation of cell division Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF18B
Gene namesi
Name:KIF18B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:27102. KIF18B.

Subcellular locationi

  • Nucleus
  • Cytoplasm
  • Cytoplasmcytoskeleton

  • Note: Present predominantly in the nucleus and to a lesser extent in the cytoplasm of interphase cells. During mitosis, found to be closely associated with astral microtubule plus ends emanating from the spindle pole during prometaphase and metaphase.

GO - Cellular componenti

  • astral microtubule Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • kinesin complex Source: GO_Central
  • microtubule end Source: HPA
  • microtubule plus-end Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Polymorphism and mutation databases

BioMutaiKIF18B.
DMDMi325511395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Kinesin-like protein KIF18BPRO_0000318969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei413 – 4131PhosphoserineCombined sources
Modified residuei438 – 4381PhosphothreonineCombined sources
Modified residuei473 – 4731PhosphoserineCombined sources
Modified residuei501 – 5011PhosphoserineCombined sources
Modified residuei645 – 6451PhosphoserineCombined sources
Modified residuei651 – 6511PhosphoserineCombined sources
Modified residuei674 – 6741PhosphoserineCombined sources
Modified residuei834 – 8341PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86Y91.
MaxQBiQ86Y91.
PaxDbiQ86Y91.
PRIDEiQ86Y91.

PTM databases

iPTMnetiQ86Y91.
PhosphoSiteiQ86Y91.

Expressioni

Tissue specificityi

Shows a prominent expression in the amygdala.1 Publication

Developmental stagei

Regulated in a cell cycle-dependent manner. Not expressed in the G1 phase. In G2, sequestered in the nucleus. Maximal levels seen at late G2/M phase and early prometaphase. Degraded at the metaphase-anaphase transition (at protein level).2 Publications

Gene expression databases

BgeeiQ86Y91.
CleanExiHS_KIF18B.
ExpressionAtlasiQ86Y91. baseline and differential.

Organism-specific databases

HPAiHPA024205.
HPA027831.

Interactioni

Subunit structurei

Interacts with MAPRE1; this interaction is required for efficient accumulation at microtubule plus ends. Interacts with KIF2C at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. KIF2C phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding.1 Publication

GO - Molecular functioni

  • kinesin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi127024. 18 interactions.
IntActiQ86Y91. 6 interactions.
MINTiMINT-4995529.
STRINGi9606.ENSP00000341466.

Structurei

3D structure databases

ProteinModelPortaliQ86Y91.
SMRiQ86Y91. Positions 17-360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 360345Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni723 – 74826KIF2C-bindingAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili375 – 40228Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi636 – 6449Nuclear localization signalCurated
Motifi665 – 6684MAPRE1-binding
Motifi786 – 7894MAPRE1-binding
Motifi812 – 8154MAPRE1-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi405 – 44541Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0242. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000231436.
HOVERGENiHBG108062.
InParanoidiQ86Y91.
OrthoDBiEOG7Z95KM.
PhylomeDBiQ86Y91.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86Y91-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHPGVTTVM AVEDSTLQVV VRVRPPTPRE LDSQRRPVVQ VVDERVLVFN
60 70 80 90 100
PEEPDGGFPG LKWGGTHDGP KKKGKDLTFV FDRVFGEAAT QQDVFQHTTH
110 120 130 140 150
SVLDSFLQGY NCSVFAYGAT GAGKTHTMLG REGDPGIMYL TTVELYRRLE
160 170 180 190 200
ARQQEKHFEV LISYQEVYNE QIHDLLEPKG PLAIREDPDK GVVVQGLSFH
210 220 230 240 250
QPASAEQLLE ILTRGNRNRT QHPTDANATS SRSHAIFQIF VKQQDRVPGL
260 270 280 290 300
TQAVQVAKMS LIDLAGSERA SSTHAKGERL REGANINRSL LALINVLNAL
310 320 330 340 350
ADAKGRKTHV PYRDSKLTRL LKDSLGGNCR TVMIAAISPS SLTYEDTYNT
360 370 380 390 400
LKYADRAKEI RLSLKSNVTS LDCHISQYAT ICQQLQAEVA ALRKKLQVYE
410 420 430 440 450
GGGQPPPQDL PGSPKSGPPP EHLPSSPLPP HPPSQPCTPE LPAGPRALQE
460 470 480 490 500
ESLGMEAQVE RAMEGNSSDQ EQSPEDEDEG PAEEVPTQMP EQNPTHALPE
510 520 530 540 550
SPRLTLQPKP VVGHFSAREL DGDRSKQLAL KVLCVAQRQY SLLQAANLLT
560 570 580 590 600
PDMITEFETL QQLVQEEKIE PGAEALRTSG LARGAPLAQE LCSESKPPGY
610 620 630 640 650
TGPVTRTMAR RLSGPLHTLG IPPGPNCTPA QGSRWPMEKK RRRPSALEAD
660 670 680 690 700
SPMAPKRGTK RQRQSFLPCL RRGSLPDTQP SQGPSTPKGE RASSPCHSPR
710 720 730 740 750
VCPATVIKSR VPLGPSAMQN CSTPLALPTR DLNATFDLSE EPPSKPSFHE
760 770 780 790 800
CIGWDKIPQE LSRLDQPFIP RAPVPLFTMK GPKPTSSLPG TSACKKKRVA
810 820 830 840 850
SSSVSHGRSR IARLPSSTLK RPAGPLVLPE LPLSPLCPSN RRNGKDLIRV
860
GRALSAGNGV TKVS
Length:864
Mass (Da):94,223
Last modified:March 8, 2011 - v3
Checksum:iEC1A26D345B8BFB1
GO
Isoform 2 (identifier: Q86Y91-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     596-596: K → IPVPSPLCPE
     830-833: ELPL → GDWH
     834-864: Missing.

Note: No experimental confirmation available.
Show »
Length:842
Mass (Da):91,965
Checksum:i1E24BBDD57D8A255
GO
Isoform 3 (identifier: Q86Y91-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     423-434: Missing.
     596-596: K → IPVPSPLCPE

Show »
Length:861
Mass (Da):93,921
Checksum:iB19CFE7979A60F92
GO
Isoform 4 (identifier: Q86Y91-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     596-596: K → IPVPSPLCPE

Show »
Length:873
Mass (Da):95,128
Checksum:i07F8B6B01D35B435
GO

Sequence cautioni

The sequence AAI36591.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI44272.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti527 – 5271Q → R.
Corresponds to variant rs17546822 [ dbSNP | Ensembl ].
VAR_038925

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei423 – 43412Missing in isoform 3. 1 PublicationVSP_040705Add
BLAST
Alternative sequencei596 – 5961K → IPVPSPLCPE in isoform 2, isoform 3 and isoform 4. 2 PublicationsVSP_040706
Alternative sequencei830 – 8334ELPL → GDWH in isoform 2. 2 PublicationsVSP_040707
Alternative sequencei834 – 86431Missing in isoform 2. 2 PublicationsVSP_040708Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU808359 mRNA. Translation: ADE43428.1.
AC015936 Genomic DNA. No translation available.
BC044933 mRNA. Translation: AAH44933.1.
BC136590 mRNA. Translation: AAI36591.1. Different initiation.
BC144271 mRNA. Translation: AAI44272.1. Different initiation.
RefSeqiNP_001251503.1. NM_001264573.1.
NP_001252506.1. NM_001265577.1.
UniGeneiHs.135094.

Genome annotation databases

EnsembliENST00000590129; ENSP00000465501; ENSG00000186185. [Q86Y91-2]
GeneIDi146909.
KEGGihsa:146909.
UCSCiuc010wjh.4. human. [Q86Y91-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU808359 mRNA. Translation: ADE43428.1.
AC015936 Genomic DNA. No translation available.
BC044933 mRNA. Translation: AAH44933.1.
BC136590 mRNA. Translation: AAI36591.1. Different initiation.
BC144271 mRNA. Translation: AAI44272.1. Different initiation.
RefSeqiNP_001251503.1. NM_001264573.1.
NP_001252506.1. NM_001265577.1.
UniGeneiHs.135094.

3D structure databases

ProteinModelPortaliQ86Y91.
SMRiQ86Y91. Positions 17-360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127024. 18 interactions.
IntActiQ86Y91. 6 interactions.
MINTiMINT-4995529.
STRINGi9606.ENSP00000341466.

PTM databases

iPTMnetiQ86Y91.
PhosphoSiteiQ86Y91.

Polymorphism and mutation databases

BioMutaiKIF18B.
DMDMi325511395.

Proteomic databases

EPDiQ86Y91.
MaxQBiQ86Y91.
PaxDbiQ86Y91.
PRIDEiQ86Y91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000590129; ENSP00000465501; ENSG00000186185. [Q86Y91-2]
GeneIDi146909.
KEGGihsa:146909.
UCSCiuc010wjh.4. human. [Q86Y91-1]

Organism-specific databases

CTDi146909.
GeneCardsiKIF18B.
H-InvDBHIX0202540.
HGNCiHGNC:27102. KIF18B.
HPAiHPA024205.
HPA027831.
MIMi614570. gene.
neXtProtiNX_Q86Y91.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0242. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129683.
HOGENOMiHOG000231436.
HOVERGENiHBG108062.
InParanoidiQ86Y91.
OrthoDBiEOG7Z95KM.
PhylomeDBiQ86Y91.

Miscellaneous databases

ChiTaRSiKIF18B. human.
GenomeRNAii146909.
NextBioi85508.
PROiQ86Y91.
SOURCEiSearch...

Gene expression databases

BgeeiQ86Y91.
CleanExiHS_KIF18B.
ExpressionAtlasiQ86Y91. baseline and differential.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cell cycle-regulated expression and subcellular localization of a kinesin-8 member human KIF18B."
    Lee Y.M., Kim E., Park M., Moon E., Ahn S.M., Kim W., Hwang K.B., Kim Y.K., Choi W., Kim W.
    Gene 466:16-25(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), IDENTIFICATION OF ISOFORM 4, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-864 (ISOFORMS 2 AND 3).
    Tissue: Testis.
  4. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-674, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413; THR-438; SER-473; SER-501; SER-645; SER-651; SER-674 AND SER-834, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413 AND SER-674, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "A complex of Kif18b and MCAK promotes microtubule depolymerization and is negatively regulated by Aurora kinases."
    Tanenbaum M.E., Macurek L., van der Vaart B., Galli M., Akhmanova A., Medema R.H.
    Curr. Biol. 21:1356-1365(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MAPRE1 AND KIF2C, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKI18B_HUMAN
AccessioniPrimary (citable) accession number: Q86Y91
Secondary accession number(s): A6NJI2
, B7ZM49, B9EGM8, D5L6I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 8, 2011
Last modified: May 11, 2016
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-10 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.